Open Access

Compound Astragalus and Salvia miltiorrhiza extract inhibits hepatocarcinogenesis via modulating TGF‑β/TβR and Imp7/8

  • Authors:
    • Chao Wu
    • Hongwei Kan
    • Min Hu
    • Xin Liu
    • Alex Boye
    • Yufeng Jiang
    • Jiajun Wu
    • Jiyu Wang
    • Xiaochuan Yang
    • Yan Yang
  • View Affiliations

  • Published online on: June 12, 2018     https://doi.org/10.3892/etm.2018.6292
  • Pages: 1052-1060
  • Copyright: © Wu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Compound Astragalus and Salvia miltiorrhiza extract (CASE) is a Chinese herbal formula consisting of astragalosides, astragalus polysaccharide and salvianolic acids extracted from Astragalus membranaceus and Salvia miltiorhiza. Previous studies by our group have demonstrated that CASE effectively suppresses diethylinitrosamine (DEN)‑induced hepatocellular carcinoma (HCC) in rats via modulating transforming growth factor β/Mothers against decapentaplegic (TGFβ/Smad) signaling. To further elucidate the mechanism of CASE, the effects of CASE on TGF‑β1, the serine/threonine kinase receptors of TGF‑β [TGF‑β receptor type‑I (TβRΙ) and TβRII] and karyopherins [Importin 7 (Imp7) and Imp8], which are crucial for TGF‑β/Smad signaling in fibro‑hepatocarcinogenesis, were assessed in the present study using in vivo (DEN‑induced HCC in rats) and in vitro [TGF‑β1‑stimulated rat myofibroblasts (MFBs) and HepG2 cells] models of fibro‑hepatocarcinogenesis. Hematoxylin and eosin staining revealed that CASE may suppress inflammatory reactions and fibrosis in HCC as well as increasing the differentiation of HCC cells. Positive TGF‑β1 staining was increased in HCC nodule areas and in adjacent normal liver tissues in DEN‑treated rats, while TβRΙ staining was increased only in normal adjacent liver tissues. The elevated expression of TGF‑β1, TβRΙ and TβRII was suppressed by CASE. CASE treatment also reduced glutathione S‑transferase P 1 and Imp7/8 protein expression in fibro‑hepatocarcinogenesis. In vitro experiments confirmed that CASE was able to decrease the expression of TβRΙ and TβRII in TGF‑β1‑stimulated MFBs and HepG2 cells. These results indicate that the anti‑HCC effect of CASE may be achieved by mediating TGF‑β/TβR and Imp7/8 protein expression, suggesting that CASE has multiple targets in HCC treatment.
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August-2018
Volume 16 Issue 2

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Wu C, Kan H, Hu M, Liu X, Boye A, Jiang Y, Wu J, Wang J, Yang X, Yang Y, Yang Y, et al: Compound Astragalus and Salvia miltiorrhiza extract inhibits hepatocarcinogenesis via modulating TGF‑β/TβR and Imp7/8. Exp Ther Med 16: 1052-1060, 2018
APA
Wu, C., Kan, H., Hu, M., Liu, X., Boye, A., Jiang, Y. ... Yang, Y. (2018). Compound Astragalus and Salvia miltiorrhiza extract inhibits hepatocarcinogenesis via modulating TGF‑β/TβR and Imp7/8. Experimental and Therapeutic Medicine, 16, 1052-1060. https://doi.org/10.3892/etm.2018.6292
MLA
Wu, C., Kan, H., Hu, M., Liu, X., Boye, A., Jiang, Y., Wu, J., Wang, J., Yang, X., Yang, Y."Compound Astragalus and Salvia miltiorrhiza extract inhibits hepatocarcinogenesis via modulating TGF‑β/TβR and Imp7/8". Experimental and Therapeutic Medicine 16.2 (2018): 1052-1060.
Chicago
Wu, C., Kan, H., Hu, M., Liu, X., Boye, A., Jiang, Y., Wu, J., Wang, J., Yang, X., Yang, Y."Compound Astragalus and Salvia miltiorrhiza extract inhibits hepatocarcinogenesis via modulating TGF‑β/TβR and Imp7/8". Experimental and Therapeutic Medicine 16, no. 2 (2018): 1052-1060. https://doi.org/10.3892/etm.2018.6292