Fluvastatin inhibits cardiomyocyte apoptosis after myocardial infarction through Toll pathway Corrigendum in /10.3892/etm.2018.6881

Jia,Lili; Wang,Liang; Liu,Wenxue; Qian,Guanglei; Jiang,Xiufang; Zhang,Zhimian

doi:10.3892/etm.2018.6297

Fluvastatin inhibits cardiomyocyte apoptosis after myocardial infarction through Toll pathway

Corrigendum in: /10.3892/etm.2018.6881

Authors:
- Lili Jia
- Liang Wang
- Wenxue Liu
- Guanglei Qian
- Xiufang Jiang
- Zhimian Zhang
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Affiliations: Qilu Hospital of Shandong University, Jinan, Shandong 250012, P.R. China, Department of Cardiology, The People's Hospital of Zhangqiu Area, Jinan, Shandong 250200, P.R. China, Department of Pediatrics, The People's Hospital of Zhangqiu Area, Jinan, Shandong 250200, P.R. China, Department of Obstetrics, The People's Hospital of Zhangqiu Area, Jinan, Shandong 250200, P.R. China, Health Examination Center, Qilu Hospital of Shandong University, Jinan, Shandong 250012, P.R. China
Published online on: June 13, 2018 https://doi.org/10.3892/etm.2018.6297
Pages: 1350-1354
Copyright: © Jia et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The present study intended to investigate the effect of fluvastatin on cardiomyocyte apoptosis after myocardial infarction in rats. Eighty myocardial infarction rat models were established and randomly divided into 4 groups (n=20): experimental group (n=20) was given fluvastatin treatment; sham operation group (n=20) and normal control group (n=20) were given saline. The dose of fluvastatin was 20 mg/(kg·d), and irrigation gavage was given for 1 week. Western blot analysis and reverse transcription-quantitative PCR (RT-qPCR) were used to detect the expression of TLR4 mRNA and protein in cardiomyocytes. TUNEL method was used to detect the apoptosis of cardiomyocytes. After fluvastatin treatment for 1 week, RT-qPCR found that compared with myocardial infarction group, the TLR4 mRNA expression of fluvastatin treatment group and normal control group was significantly increased, and the differences between groups were a statistically significant difference (p<0.05). Western blot analysis showed that compared with the myocardial infarction group, the expression of TLR4 protein in normal control group, sham operation group and fluvastatin treatment group were significantly decreased, and they all were statistically significant (p<0.05). TUNEL method found that compared with the myocardial infarction group, the fluvastatin treatment group could significantly reduce the apoptosis of cardiomyocytes (19.2±3.8%), and the difference was statistically significant (p<0.05). Fluvastatin can inhibit myocardial infarction and decrease cardiomyocyte apoptosis by increasing the expression of TLR4-like receptor.

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1	Thiene G, Corrado D and Basso C: Sudden cardiac death in the young and athletes. Springer Milan. 2016:21–71. 2016.
2	Sankar NM, Ramani SS and Anantharaman R: Coronary artery disease in women. Indian Heart J. 69:807–808. 2017. View Article : Google Scholar : PubMed/NCBI
3	Forman DE, Alexander K, Brindis RG, Curtis AB, Maurer M, Rich MW, Sperling L and Wenger NK: Improved cardiovascular disease outcomes in older adults. F1000Res. 5:1–9. 2016.
4	Zhou M, Wang H, Zhu J, Chen W, Wang L, Liu S, Li Y, Wang L, Liu Y, Yin P, et al: Cause-specific mortality for 240 causes in China during 1990–2013: A systematic subnational analysis for the Global Burden of Disease Study 2013. Lancet. 387:251–272. 2016. View Article : Google Scholar : PubMed/NCBI
5	Chan NY: Sudden cardiac death in Asia and China: Are we different? J Am Coll Cardiol. 67:590–592. 2016. View Article : Google Scholar : PubMed/NCBI
6	Reed GW, Rossi JE and Cannon CP: Acute myocardial infarction. Lancet. 389:197–210. 2017. View Article : Google Scholar : PubMed/NCBI
7	Stewart WJ: Atrial myocardial infarction: A neglected stalker in coronary patients. J Am Coll Cardiol. 70:2890–2892. 2017. View Article : Google Scholar : PubMed/NCBI
8	Li S, Guo LZ, Kim MH, Han JY and Serebruany V: Platelet microRNA for predicting acute myocardial infarction. J Thromb Thrombolysis. 44:556–564. 2017. View Article : Google Scholar : PubMed/NCBI
9	Kataoka Y, Andrews J, Puri R, Psaltis P and Nicholls SJ: Lipid lowering therapy to modify plaque microstructures. J Atheroscler Thromb. 24:360–372. 2017. View Article : Google Scholar : PubMed/NCBI
10	Ruel I, Aljenedil S, Sadri I, de Varennes É, Hegele RA, Couture P, Bergeron J, Wanneh E, Baass A, Dufour R, et al: Imputation of baseline LDL cholesterol concentration in patients with familial hypercholesterolemia on statins or ezetimibe. Clin Chem. 70:20222017.
11	Zhai Y, Ao L, Cleveland JC, Zeng Q, Reece TB, Fullerton DA and Meng X: Toll-like receptor 4 mediates the inflammatory responses and matrix protein remodeling in remote non-ischemic myocardium in a mouse model of myocardial ischemia and reperfusion. PLoS One. 10:e01218532015. View Article : Google Scholar : PubMed/NCBI
12	Czabotar PE, Lessene G, Strasser A and Adams JM: Control of apoptosis by the BCL-2 protein family: Implications for physiology and therapy. Nat Rev Mol Cell Biol. 15:49–63. 2014. View Article : Google Scholar : PubMed/NCBI
13	Wang X, Sun Y, Yang H, Lu Y and Li L: Oxidized low-density lipoprotein induces apoptosis in cultured neonatal rat cardiomyocytes by modulating the TLR4/NF-κB pathway. Sci Rep. 6:278662016. View Article : Google Scholar : PubMed/NCBI
14	Tixeira R, Caruso S, Paone S, Baxter AA, Atkin-Smith GK, Hulett MD and Poon IK: Defining the morphologic features and products of cell disassembly during apoptosis. Apoptosis. 22:475–477. 2017. View Article : Google Scholar : PubMed/NCBI
15	Chen Z, Wu Z, Huang C, Zhao Y, Zhou Y, Zhou X, Lu X, Mao L and Li S: Effect of lipoxin A4 on myocardial ischemia reperfusion injury following cardiac arrest in a rabbit model. Inflammation. 36:468–475. 2013. View Article : Google Scholar : PubMed/NCBI
16	Kleinbongard P, Schulz R and Heusch G: TNFα in myocardial ischemia/reperfusion, remodeling and heart failure. Heart Fail Rev. 16:49–69. 2011. View Article : Google Scholar : PubMed/NCBI
17	Liu Q, Zhang J, Xu Y, Huang Y and Wu C: Effect of carvedilol on cardiomyocyte apoptosis in a rat model of myocardial infarction: A role for toll-like receptor 4. Indian J Pharmacol. 45:458–463. 2013. View Article : Google Scholar : PubMed/NCBI
18	Kim SC, Stice JP, Chen L, Jung JS, Gupta S, Wang Y, Baumgarten G, Trial J and Knowlton AA: Extracellular heat shock protein 60, cardiac myocytes, and apoptosis. Circ Res. 105:1186–1195. 2009. View Article : Google Scholar : PubMed/NCBI
19	Satoh M, Shimoda Y, Maesawa C, Akatsu T, Ishikawa Y, Minami Y, Hiramori K and Nakamura M: Activated toll-like receptor 4 in monocytes is associated with heart failure after acute myocardial infarction. Int J Cardiol. 109:226–234. 2006. View Article : Google Scholar : PubMed/NCBI
20	Zhai Y, Shen XD, OConnell R, Gao F, Lassman C, Busuttil RW, Cheng G and Kupiec-Weglinski JW: Cutting edge: TLR4 activation mediates liver ischemia/reperfusion inflammatory response via IFN regulatory factor 3-dependent MyD88-independent pathway. J Immunol. 173:7115–7119. 2004. View Article : Google Scholar : PubMed/NCBI
21	Li Z, Wang L, Hu X, Zhang P, Chen Y, Liu X, Xu M, Zhang Y and Zhang M: Effect of rosuvastatin on atherosclerotic plaque stability: An intravascular ultrasound elastography study. Atherosclerosis. 248:27–35. 2016. View Article : Google Scholar : PubMed/NCBI
22	Yang J, Yang J, Ding JW, Chen LH, Wang YL, Li S and Wu H: Sequential expression of TLR4 and its effects on the myocardium of rats with myocardial ischemia-reperfusion injury. Inflammation. 31:304–312. 2008. View Article : Google Scholar : PubMed/NCBI
23	Liu L, Wang Y, Cao ZY, Wang MM, Liu XM, Gao T, Hu QK, Yuan WJ and Lin L: Up-regulated TLR4 in cardiomyocytes exacerbates heart failure after long-term myocardial infarction. J Cell Mol Med. 19:2728–2740. 2015. View Article : Google Scholar : PubMed/NCBI
24	Sun Y, Huang J and Song K: BET protein inhibition mitigates acute myocardial infarction damage in rats via the TLR4/TRAF6/NF-κB pathway. Exp Ther Med. 10:2319–2324. 2015. View Article : Google Scholar : PubMed/NCBI