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Article Open Access

Silence of IGFBP7 suppresses apoptosis and epithelial mesenchymal transformation of high glucose induced‑podocytes

  • Authors:
    • Xiaojun Cai
    • Lei Wang
    • Xuling Wang
    • Fengyan Hou
  • View Affiliations / Copyright

    Affiliations: Department of Endocrinology, Heilongjiang Provincial Academy of Chinese Medical Science, Harbin, Heilongjiang 150036, P.R. China
    Copyright: © Cai et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 1095-1102
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    Published online on: June 13, 2018
       https://doi.org/10.3892/etm.2018.6298
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Abstract

Insulin‑like growth factor‑binding protein 7 (IGFBP7) has been identified as a secreted protein associated with a number of cellular processes. However, the specific regulatory mechanisms of IGFBP7 on podocytes of diabetic nephropathy (DN) are yet to be elucidated. In the present study, podocytes were identified initially via an immunofluorescence assay using an anti‑synaptopodin antibody. It was subsequently demonstrated that glucose promoted podocyte proliferation in a time‑ and dose‑dependent manner via MTT assay. In addition, IGFBP7 expression was silenced in podocytes via siRNA, the effects of which were evaluated using western blotting and reverse transcription‑quantitative polymerase chain reaction. It was demonstrated that silencing IGFBP7 inhibited apoptosis and epithelial mesenchymal transformation (EMT) of podocytes mediated by high glucose (HG). Transforming growth factor (TGF)‑β1/mothers against decapentaplegic homolog (Smad) signaling was associated with proliferation, apoptotic activities and EMT. Therefore, the expression levels of TGF‑β1/Smad pathway were detected, and it was observed that silencing IGFBP7 suppressed the TGF‑β1/Smad pathway in podocytes induced by HG. These findings suggested that IGFBP7 may serve as a potential therapeutic target for DN.
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Copy and paste a formatted citation
Spandidos Publications style
Cai X, Wang L, Wang X and Hou F: Silence of IGFBP7 suppresses apoptosis and epithelial mesenchymal transformation of high glucose induced‑podocytes. Exp Ther Med 16: 1095-1102, 2018.
APA
Cai, X., Wang, L., Wang, X., & Hou, F. (2018). Silence of IGFBP7 suppresses apoptosis and epithelial mesenchymal transformation of high glucose induced‑podocytes. Experimental and Therapeutic Medicine, 16, 1095-1102. https://doi.org/10.3892/etm.2018.6298
MLA
Cai, X., Wang, L., Wang, X., Hou, F."Silence of IGFBP7 suppresses apoptosis and epithelial mesenchymal transformation of high glucose induced‑podocytes". Experimental and Therapeutic Medicine 16.2 (2018): 1095-1102.
Chicago
Cai, X., Wang, L., Wang, X., Hou, F."Silence of IGFBP7 suppresses apoptosis and epithelial mesenchymal transformation of high glucose induced‑podocytes". Experimental and Therapeutic Medicine 16, no. 2 (2018): 1095-1102. https://doi.org/10.3892/etm.2018.6298
Copy and paste a formatted citation
x
Spandidos Publications style
Cai X, Wang L, Wang X and Hou F: Silence of IGFBP7 suppresses apoptosis and epithelial mesenchymal transformation of high glucose induced‑podocytes. Exp Ther Med 16: 1095-1102, 2018.
APA
Cai, X., Wang, L., Wang, X., & Hou, F. (2018). Silence of IGFBP7 suppresses apoptosis and epithelial mesenchymal transformation of high glucose induced‑podocytes. Experimental and Therapeutic Medicine, 16, 1095-1102. https://doi.org/10.3892/etm.2018.6298
MLA
Cai, X., Wang, L., Wang, X., Hou, F."Silence of IGFBP7 suppresses apoptosis and epithelial mesenchymal transformation of high glucose induced‑podocytes". Experimental and Therapeutic Medicine 16.2 (2018): 1095-1102.
Chicago
Cai, X., Wang, L., Wang, X., Hou, F."Silence of IGFBP7 suppresses apoptosis and epithelial mesenchymal transformation of high glucose induced‑podocytes". Experimental and Therapeutic Medicine 16, no. 2 (2018): 1095-1102. https://doi.org/10.3892/etm.2018.6298
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