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Detection of Treg/Th17 cells and related cytokines in peripheral blood of chronic hepatitis B patients combined with thrombocytopenia and the clinical significance

  • Authors:
    • Yinghui Wang
    • Liru Wang
    • Wei Gao
    • Xi Chen
    • Yanhua Su
  • View Affiliations / Copyright

    Affiliations: Department of Hematology, The First Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang 150036, P.R. China, Department of Hematology, Heilongjiang Provincial Hospital, Harbin, Heilongjiang 150036, P.R. China, Department of Infectious Diseases, Heilongjiang Provincial Hospital, Harbin, Heilongjiang 150036, P.R. China, Department of Hematology, The Second Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang 150036, P.R. China, Department of Hematology, The First Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang 150036, P.R. China
  • Pages: 1328-1332
    |
    Published online on: June 14, 2018
       https://doi.org/10.3892/etm.2018.6311
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Abstract

Changes of regulatory T (Treg) cells and Th17 cells, and related cytokines in peripheral blood of patients with chronic hepatitis B combined with thrombocytopenia were investigated to explore the relationship with treatment outcomes. A total of 45 chronic hepatitis B patients combined with thrombocytopenia were selected in Heilongjiang Provincial Hospital from June 2015 to December 2016. All patients were treated with prednisolone acetate + γ globulins for 60 days. Treg cells and Th17 cells in peripheral blood were detected by flow cytometry, and IL-10, TGF-β, IL-17, IL-21 and IL-22 in peripheral blood were detected by ELISA before and after treatment. No significant differences in the percentages of Treg and Th17, and levels of IL-10, TGF-β, IL-17, IL-21 and IL-22 were found in non-responders (n=17, platelets <100x109/l) before and after treatment (P>0.05). After treatment, percentage of Treg was significantly increased (higher than that of non-responders) and percentage of Th17 was significantly decreased (lower than that of non-responders) in responders (P<0.05). In addition, serum levels of IL-10 and TGF-β were significantly increased (higher than that of non-responders) and serum levels of IL-17, IL-21 and IL-22 were significantly decreased (lower than that of non-responders) in responders (P<0.05). The results showed that after treatment, the number of Treg cells was increased, the number of Th17 cells was decreased, the levels of anti-inflammatory factors IL-10 and TGF-β were increased, and levels of pro-inflammatory factors IL-17, IL-21 and IL-22 were decreased in chronic hepatitis B patients combined with thrombocytopenia, indicating the decreased autoimmune response and improved thrombocytopenia. The changes were closely related to the complete response.
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1 

Peck-Radosavljevic M: Thrombocytopenia in liver disease. Can J Gastroenterol. 14:60D–66D. 2000. View Article : Google Scholar : PubMed/NCBI

2 

Thomopoulos KC, Labropoulou-Karatza C, Mimidis KP, Katsakoulis EC, Iconomou G and Nikolopoulou VN: Non-invasive predictors of the presence of large oesophageal varices in patients with cirrhosis. Dig Liver Dis. 35:473–478. 2003. View Article : Google Scholar : PubMed/NCBI

3 

Aref S, Mabed M, Selim T, Goda T and Khafagy N: Thrombopoietin (TPO) levels in hepatic patients with thrombocytopenia. Hematology. 9:351–356. 2004. View Article : Google Scholar : PubMed/NCBI

4 

Afdhal N, McHutchison J, Brown R, Jacobson I, Manns M, Poordad F, Weksler B and Esteban R: Thrombocytopenia associated with chronic liver disease. J Hepatol. 48:1000–1007. 2008. View Article : Google Scholar : PubMed/NCBI

5 

Stornaiuolo G, Amato A and Gaeta GB: Adefovir dipivoxil-associated thrombocytopenia in a patient with chronic hepatitis B. Dig Liver Dis. 38:211–212. 2006. View Article : Google Scholar : PubMed/NCBI

6 

Rodeghiero F, Stasi R, Gernsheimer T, Michel M, Provan D, Arnold DM, Bussel JB, Cines DB, Chong BH, Cooper N, et al: Standardization of terminology, definitions and outcome criteria in immune thrombocytopenic purpura of adults and children: Report from an international working group. Blood. 113:pp. 2386–2393. 2009, View Article : Google Scholar : PubMed/NCBI

7 

Shevach EM: Mechanisms of foxp3+ T regulatory cell-mediated suppression. Immunity. 30:636–645. 2009. View Article : Google Scholar : PubMed/NCBI

8 

Wing K, Onishi Y, Prieto-Martin P, Yamaguchi T, Miyara M, Fehervari Z, Nomura T and Sakaguchi S: CTLA-4 control over Foxp3+ regulatory T cell function. Science. 322:271–275. 2008. View Article : Google Scholar : PubMed/NCBI

9 

Mittal SK and Roche PA: Suppression of antigen presentation by IL-10. Curr Opin Immunol. 34:22–27. 2015. View Article : Google Scholar : PubMed/NCBI

10 

Keswani T, Sarkar S, Sengupta A and Bhattacharyya A: Role of TGF-β and IL-6 in dendritic cells, Treg and Th17 mediated immune response during experimental cerebral malaria. Cytokine. 88:154–166. 2016. View Article : Google Scholar : PubMed/NCBI

11 

Liu J, Hong X, Lin D, Luo X, Zhu M and Mo H: Artesunate influences Th17/Treg lymphocyte balance by modulating Treg apoptosis and Th17 proliferation in a murine model of rheumatoid arthritis. Exp Ther Med. 13:2267–2273. 2017. View Article : Google Scholar : PubMed/NCBI

12 

Shrivastava S, TrehanPati N, Patra S, Kottilil S, Pande C, Trivedi SS and Sarin SK: Increased regulatory T cells and impaired functions of circulating CD8 T lymphocytes is associated with viral persistence in Hepatitis B virus-positive newborns. J Viral Hepat. 20:582–591. 2013. View Article : Google Scholar : PubMed/NCBI

13 

Feng S, Chen XM, Wang JF and Xu XQ: Th17 cells associated cytokines and cancer. Eur Rev Med Pharmacol Sci. 20:4032–4040. 2016.PubMed/NCBI

14 

Harrington LE, Hatton RD, Mangan PR, Turner H, Murphy TL, Murphy KM and Weaver CT: Interleukin 17-producing CD4+ effector T cells develop via a lineage distinct from the T helper type 1 and 2 lineages. Nat Immunol. 6:1123–1132. 2005. View Article : Google Scholar : PubMed/NCBI

15 

Li J, Lai X, Liao W, He Y, Liu Y and Gong J: The dynamic changes of Th17/Treg cytokines in rat liver transplant rejection and tolerance. Int Immunopharmacol. 11:962–967. 2011. View Article : Google Scholar : PubMed/NCBI

16 

Ni K, Zhao L, Wu J, Chen W, Hongya Yang and Li X: Th17/Treg balance in children with obstructive sleep apnea syndrome and the relationship with allergic rhinitis. Int J Pediatr Otorhinolaryngol. 79:1448–1454. 2015. View Article : Google Scholar : PubMed/NCBI

17 

Zheng Y, Wang Z, Deng L, Zhang G, Yuan X, Huang L, Xu W and Shen L: Modulation of STAT3 and STAT5 activity rectifies the imbalance of Th17 and Treg cells in patients with acute coronary syndrome. Clin Immunol. 157:65–77. 2015. View Article : Google Scholar : PubMed/NCBI

18 

Kleinewietfeld M and Hafler DA: The plasticity of human Treg and Th17 cells and its role in autoimmunity. Semin Immunol. 25:305–312. 2013. View Article : Google Scholar : PubMed/NCBI

19 

Su ZJ, Yu XP, Guo RY, Ming DS, Huang LY, Su ML, Deng Y and Lin ZZ: Changes in the balance between Treg and Th17 cells in patients with chronic hepatitis B. Diagn Microbiol Infect Dis. 76:437–444. 2013. View Article : Google Scholar : PubMed/NCBI

20 

Li K, Liu H and Guo T: Th17/Treg imbalance is an indicator of liver cirrhosis process and a risk factor for HCC occurrence in HBV patients. Clin Res Hepatol Gastroenterol. 41:399–407. 2017. View Article : Google Scholar : PubMed/NCBI

21 

Gilli SC, de Souza Medina S, de Castro V, Fernandes LG and Saad ST: Platelet associated IgG may be related with thrombocytopenia in patients with myelodysplastic syndromes. Leuk Res. 36:554–559. 2012. View Article : Google Scholar : PubMed/NCBI

22 

Aboul-Fotoh L-M, Abdel Raheem MM, El-Deen MA and Osman AM: Role of CD4+CD25+ T cells in children with idiopathic thrombocytopenic purpura. J Pediatr Hematol Oncol. 33:81–85. 2011. View Article : Google Scholar : PubMed/NCBI

23 

Liu B, Gao W, Zhang L, Wang J, Chen M, Peng M, Ren H and Hu P: Th17/Treg imbalance and increased interleukin-21 are associated with liver injury in patients with chronic severe hepatitis B. Int Immunopharmacol. 46:48–55. 2017. View Article : Google Scholar : PubMed/NCBI

24 

Cao J, Chen C, Zeng L, Li L, Li X, Li Z and Xu K: Elevated plasma IL-22 levels correlated with Th1 and Th22 cells in patients with immune thrombocytopenia. Clin Immunol. 141:121–123. 2011. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Wang Y, Wang L, Gao W, Chen X and Su Y: Detection of Treg/Th17 cells and related cytokines in peripheral blood of chronic hepatitis B patients combined with thrombocytopenia and the clinical significance. Exp Ther Med 16: 1328-1332, 2018.
APA
Wang, Y., Wang, L., Gao, W., Chen, X., & Su, Y. (2018). Detection of Treg/Th17 cells and related cytokines in peripheral blood of chronic hepatitis B patients combined with thrombocytopenia and the clinical significance. Experimental and Therapeutic Medicine, 16, 1328-1332. https://doi.org/10.3892/etm.2018.6311
MLA
Wang, Y., Wang, L., Gao, W., Chen, X., Su, Y."Detection of Treg/Th17 cells and related cytokines in peripheral blood of chronic hepatitis B patients combined with thrombocytopenia and the clinical significance". Experimental and Therapeutic Medicine 16.2 (2018): 1328-1332.
Chicago
Wang, Y., Wang, L., Gao, W., Chen, X., Su, Y."Detection of Treg/Th17 cells and related cytokines in peripheral blood of chronic hepatitis B patients combined with thrombocytopenia and the clinical significance". Experimental and Therapeutic Medicine 16, no. 2 (2018): 1328-1332. https://doi.org/10.3892/etm.2018.6311
Copy and paste a formatted citation
x
Spandidos Publications style
Wang Y, Wang L, Gao W, Chen X and Su Y: Detection of Treg/Th17 cells and related cytokines in peripheral blood of chronic hepatitis B patients combined with thrombocytopenia and the clinical significance. Exp Ther Med 16: 1328-1332, 2018.
APA
Wang, Y., Wang, L., Gao, W., Chen, X., & Su, Y. (2018). Detection of Treg/Th17 cells and related cytokines in peripheral blood of chronic hepatitis B patients combined with thrombocytopenia and the clinical significance. Experimental and Therapeutic Medicine, 16, 1328-1332. https://doi.org/10.3892/etm.2018.6311
MLA
Wang, Y., Wang, L., Gao, W., Chen, X., Su, Y."Detection of Treg/Th17 cells and related cytokines in peripheral blood of chronic hepatitis B patients combined with thrombocytopenia and the clinical significance". Experimental and Therapeutic Medicine 16.2 (2018): 1328-1332.
Chicago
Wang, Y., Wang, L., Gao, W., Chen, X., Su, Y."Detection of Treg/Th17 cells and related cytokines in peripheral blood of chronic hepatitis B patients combined with thrombocytopenia and the clinical significance". Experimental and Therapeutic Medicine 16, no. 2 (2018): 1328-1332. https://doi.org/10.3892/etm.2018.6311
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