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Case Report Open Access

Personalized genomic analysis based on circulating tumor cells of extra‑skeletal Ewing sarcoma of the uterus: A case report of a 16‑year‑old Korean female

  • Authors:
    • Sun‑Young Lee
    • Sery Lim
    • Dong‑Hyu Cho
  • View Affiliations / Copyright

    Affiliations: Department of Radiation Oncology, Chonbuk National University Hospital, Chonbuk National University Medical School, Jeonju, Jeollabukdo 561‑712, Republic of Korea, Department of Molecular and Life Science, Hanyang University, Ansan‑si, Gyeonggi 426‑791, Republic of Korea, Research Institute of Clinical Medicine of Chonbuk National University-Biomedical Research Institute of Chonbuk National University Hospital, Jeonju, Jeollabukdo 561‑712, Republic of Korea
    Copyright: © Lee et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 1343-1349
    |
    Published online on: June 19, 2018
       https://doi.org/10.3892/etm.2018.6323
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Abstract

A 16‑year‑old female with Ewing sarcoma, a very rare disease with poor prognosis in women, was admitted to the hospital with abdominal pain. Diagnostic laparotomy revealed the Ewing sarcoma originating from the extramural uterus. Histological examination yielded positive test results for CD99, vimentin, S‑100, eosin 5‑maleimide and periodic acid‑Shiff. EWS‑FLI1 type 1 translocation was confirmed. Fibroblast growth factor receptor (FGFR) 4 (c.1162G> A) and HRas proto‑oncogene (HRAS; c.182A> G) mutations were also detected. At eight months following complete remission, pelvic lymph node metastasis was confirmed. Epithelial cell adhesion molecule‑positive circulating tumor cells (CTCs) were detected in liquid biopsy. FGFR3 (c.1948A> G) and FGFR4 (c.1162G> A) mutations were found in the CTCs. FGFR4 (c.1162G> A) and HRAS (c.182A> G) mutations were confirmed in cell‑free circulating tumor DNA. A sequence of EWSR1 gene was also confirmed in the CTCs. To the best of our knowledge, this is the first report of skeletal Ewing sarcoma being detected using multiple noninvasive diagnostic methods to observe genetic translocation and mutation in blood CTCs. It may be used to monitor the therapeutic effect of cancer or predict prognosis. Therefore, liquid biopsy is expected to serve a pivotal role in ultra‑precise medicine in the future.
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1 

Burt M, Karpeh M, Ukoha O, Bains MS, Martini N, McCormack PM, Rusch VW and Ginsberg RJ: Medical tumors of the chest wall. Solitary plasmacytoma and Ewing's sarcoma. J Thorac Cardiovasc Surg. 105:89–96. 1993.

2 

Loverro G, Resta L, Di Naro E, Caringella AM, Mastrolia SA, Vicino M, Tartagni M and Schonauer LM: Conservative treatment of Ewing's sarcoma of the uterus in young women. Case Rep Obstet Gynecol. 2015:8718212015.PubMed/NCBI

3 

Jawad MU, Cheung MC, Min ES, Schneiderbauer MM, Koniaris LG and Scully SP: Ewing sarcoma demonstrates racial disparities in incidence-related and sex-related differences in outcome: An analysis of 1631 cases from the SEER database, 1973-2005. Cancer. 115:3526–3536. 2009. View Article : Google Scholar : PubMed/NCBI

4 

Brasme JF, Chalumeau M, Oberlin O, Valteau-Couanet D and Gaspar N: Time to diagnosis of Ewing tumors in children and adolescents is not associated with metastasis or survival: A prospective multicenter study of 436 patients. J Clin Oncol. 32:1935–1940. 2014. View Article : Google Scholar : PubMed/NCBI

5 

Owen LA, Kowalewski AA and Lessnick SL: EWS/FLI mediates transcriptional repression via NKX2.2 during oncogenic transformation in Ewing's sarcoma. PLoS One. 3:e19652008. View Article : Google Scholar : PubMed/NCBI

6 

Esiashvili N, Goodman M and Marcus RB Jr: Changes in incidence and survival of Ewing sarcoma patients over the past 3 decades: Surveillance epidemiology and end results data. J Pediatr Hematol Oncol. 30:425–430. 2008. View Article : Google Scholar : PubMed/NCBI

7 

Shapiro B, Chakrabarty M, Cohn EM and Leon SA: Determination of circulating DNA levels in patients with benign or malignant gastrointestinal disease. Cancer. 51:2116–2120. 1983. View Article : Google Scholar : PubMed/NCBI

8 

Crowley E, Di Nicolantonio F, Loupakis F and Bardelli A: Liquid biopsy: Monitoring cancer-genetics in the blood. Nat Rev Clin Oncol. 10:472–484. 2013. View Article : Google Scholar : PubMed/NCBI

9 

Kim TH, Lim M, Park J, Oh JM, Kim H, Jeong H, Lee SJ, Park HC, Jung S, Kim BC, et al: FAST: Size-selective, clog-free isolation of rare cancer cells from whole blood at a liquid-liquid interface. Anal Chem. 89:1155–1162. 2017. View Article : Google Scholar : PubMed/NCBI

10 

Mackall CL, Meltzer PS and Helman LJ: Focus on sarcomas. Cancer Cell. 2:175–178. 2002. View Article : Google Scholar : PubMed/NCBI

11 

Pennacchioli E, Tosti G, Barberis M, De Pas TM, Verrecchia F, Menicanti C, Testori A and Mazzarol G: Sarcoma spreads primarily through the vascular system: Are there biomarkers associated with vascular spread? Clin Exp Metastasis. 29:757–773. 2012. View Article : Google Scholar : PubMed/NCBI

12 

Tellez-Gabriel M, Brown HK, Young R, Heymann MF and Heymann D: The challenges of detecting circulating tumor cells in sarcoma. Front Oncol. 6:2022016. View Article : Google Scholar : PubMed/NCBI

13 

Aryee DN, Niedan S, Kauer M, Schwentner R, Bennani-Baiti IM, Ban J, Muehlbacher K, Kreppel M, Walker RL, Meltzer P, et al: Hypoxia modulates EWS-FLI1 transcriptional signature and enhances the malignant properties of Ewing's sarcoma cells in vitro. Cancer Res. 70:4015–4023. 2010. View Article : Google Scholar : PubMed/NCBI

14 

Lewis TB, Coffin CM and Bernard PS: Differentiating Ewing's sarcoma from other round blue cell tumors using a RT-PCR translocation panel on formalin-fixed paraffin-embedded tissues. Mod Pathol. 20:397–404. 2007. View Article : Google Scholar : PubMed/NCBI

15 

Shukla N, Ameur N, Yilmaz I, Nafa K, Lau CY, Marchetti A, Borsu L, Barr FG and Ladanyi M: Oncogene mutation profiling of pediatric solid tumors reveals significant subsets of embryonal rhabdomyosarcoma and neuroblastoma with mutated genes in growth signaling pathways. Clin Cancer Res. 18:748–757. 2012. View Article : Google Scholar : PubMed/NCBI

16 

Cates JM and Coffin CM: Neurogenic tumors of soft tissue. Pediatr Dev Pathol. 15 1 Suppl:S62–S107. 2012. View Article : Google Scholar

17 

Park JY, Lee S, Kang HJ, Kim HS and Park SY: Primary Ewing's sarcoma-primitive neuroectodermal tumor of the uterus: A case report and literature review. Gynecol Oncol. 106:427–432. 2007. View Article : Google Scholar : PubMed/NCBI

18 

Fadare O: Uncommon sarcomas of the uterine cervix: A review of selected entities. Diagn Pathol. 1:302006. View Article : Google Scholar : PubMed/NCBI

19 

Yi T, Wang P, Lin L and Jiang W: Ewing's sarcoma/peripheral primitive neuroectodermal tumors of the uterus confirmed with fluorescence in situ hybridization in a 29-year-old Chinese female: A case report and published work review. J Obstet Gynaecol Res. 41:478–482. 2015. View Article : Google Scholar : PubMed/NCBI

20 

Iwamoto Y: Diagnosis and treatment of Ewing's sarcoma. Jpn J Clin Oncol. 37:79–89. 2007. View Article : Google Scholar : PubMed/NCBI

21 

Couturier J: Soft tissue tumors: Ewing's tumors/Primitive neurectodermal tumors (PNET). Atlas Genet Cytogenet Oncol Haematol. 2:148–150. 1998.

22 

Turc-Carel C, Aurias A, Mugneret F, Lizard S, Sidaner I, Volk C, Thiery JP, Olschwang S, Philip I, Berger MP, et al: Chromosomes in Ewing's sarcoma. I. An evaluation of 85 cases of remarkable consistency of t(11;22)(q24;q12). Cancer Genet Cytogenet. 32:229–238. 1988.

23 

Weiss S and Goldblum J: Extraskeletal Ewing's sarcoma/primitive neuroectodermal tumor family. In: Enzinger and Weiss's soft tissue tumors. 5th. Weiss SW and Goldblum JR: Mosby, St Louis, MO: pp. 963–979. 2007

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Copy and paste a formatted citation
Spandidos Publications style
Lee SY, Lim S and Cho DH: Personalized genomic analysis based on circulating tumor cells of extra‑skeletal Ewing sarcoma of the uterus: A case report of a 16‑year‑old Korean female. Exp Ther Med 16: 1343-1349, 2018.
APA
Lee, S., Lim, S., & Cho, D. (2018). Personalized genomic analysis based on circulating tumor cells of extra‑skeletal Ewing sarcoma of the uterus: A case report of a 16‑year‑old Korean female. Experimental and Therapeutic Medicine, 16, 1343-1349. https://doi.org/10.3892/etm.2018.6323
MLA
Lee, S., Lim, S., Cho, D."Personalized genomic analysis based on circulating tumor cells of extra‑skeletal Ewing sarcoma of the uterus: A case report of a 16‑year‑old Korean female". Experimental and Therapeutic Medicine 16.2 (2018): 1343-1349.
Chicago
Lee, S., Lim, S., Cho, D."Personalized genomic analysis based on circulating tumor cells of extra‑skeletal Ewing sarcoma of the uterus: A case report of a 16‑year‑old Korean female". Experimental and Therapeutic Medicine 16, no. 2 (2018): 1343-1349. https://doi.org/10.3892/etm.2018.6323
Copy and paste a formatted citation
x
Spandidos Publications style
Lee SY, Lim S and Cho DH: Personalized genomic analysis based on circulating tumor cells of extra‑skeletal Ewing sarcoma of the uterus: A case report of a 16‑year‑old Korean female. Exp Ther Med 16: 1343-1349, 2018.
APA
Lee, S., Lim, S., & Cho, D. (2018). Personalized genomic analysis based on circulating tumor cells of extra‑skeletal Ewing sarcoma of the uterus: A case report of a 16‑year‑old Korean female. Experimental and Therapeutic Medicine, 16, 1343-1349. https://doi.org/10.3892/etm.2018.6323
MLA
Lee, S., Lim, S., Cho, D."Personalized genomic analysis based on circulating tumor cells of extra‑skeletal Ewing sarcoma of the uterus: A case report of a 16‑year‑old Korean female". Experimental and Therapeutic Medicine 16.2 (2018): 1343-1349.
Chicago
Lee, S., Lim, S., Cho, D."Personalized genomic analysis based on circulating tumor cells of extra‑skeletal Ewing sarcoma of the uterus: A case report of a 16‑year‑old Korean female". Experimental and Therapeutic Medicine 16, no. 2 (2018): 1343-1349. https://doi.org/10.3892/etm.2018.6323
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