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Role of vasodilator‑stimulated phosphoprotein in human cytomegalovirus‑induced hyperpermeability of human endothelial cells

  • Authors:
    • Yihao Tian
    • Yanqi He
    • Ling Zhang
    • Jie Zhang
    • Liu Xu
    • Yanbin Ma
    • Xiaolong Xu
    • Lei Wei
  • View Affiliations / Copyright

    Affiliations: Department of Human Anatomy, Histology and Embryology, School of Basic Medical Sciences, Wuhan University, Wuhan, Hubei 430071, P.R. China, Hubei Provincial Key Laboratory of Developmentally Originated Disease, Department of Pathology and Pathophysiology, School of Basic Medical Sciences, Wuhan University, Wuhan, Hubei 430071, P.R. China
    Copyright: © Tian et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 1295-1303
    |
    Published online on: June 20, 2018
       https://doi.org/10.3892/etm.2018.6332
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Abstract

Atherosclerosis (AS) is a common chronic vascular disease and epidemiological evidence demonstrates that infection is closely associated with the occurrence of AS, including infection by human cytomegalovirus (HCMV) and Chlamydophila pneumoniae. The aim of the present study was to investigate the effect of HCMV AD169 infection on the barrier function of human umbilical vein endothelial cells (HUVECs) and to understand the role of vasodilator‑stimulated phosphoprotein (VASP) during this process. In cultured HUVEC‑CRL‑1730 cells, knockdown of VASP expression with small interfering (si)RNA‑VASP resulted in impaired cellular barrier function. Furthermore, knockdown of Ras‑related C3 botulinum toxin substrate 1 (Rac1) using siRNA‑Rac1 could induce downregulation of VASP expression in HUVEC‑CRL‑1730 cells. Additionally, following the infection of the cells by HCMV, cellular morphological alterations could be observed under an inverted microscope, the mRNA and protein levels of Rac1 and VASP were transiently reduced, and what appeared to be a time‑dependent impairment of the barrier function was observed. Finally, transfection of siRNA‑VASP or siRNA‑Rac1 into HCMV‑infected HUVEC‑CRL‑1730 cells resulted in increased impairment of the cellular barrier function. Taken together, these data demonstrated that HCMV infection could induce impairment of the barrier function in monolayer HUVEC‑CRL‑1730 cells via interference with Rac1/VASP expression.
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Copy and paste a formatted citation
Spandidos Publications style
Tian Y, He Y, Zhang L, Zhang J, Xu L, Ma Y, Xu X and Wei L: Role of vasodilator‑stimulated phosphoprotein in human cytomegalovirus‑induced hyperpermeability of human endothelial cells. Exp Ther Med 16: 1295-1303, 2018.
APA
Tian, Y., He, Y., Zhang, L., Zhang, J., Xu, L., Ma, Y. ... Wei, L. (2018). Role of vasodilator‑stimulated phosphoprotein in human cytomegalovirus‑induced hyperpermeability of human endothelial cells. Experimental and Therapeutic Medicine, 16, 1295-1303. https://doi.org/10.3892/etm.2018.6332
MLA
Tian, Y., He, Y., Zhang, L., Zhang, J., Xu, L., Ma, Y., Xu, X., Wei, L."Role of vasodilator‑stimulated phosphoprotein in human cytomegalovirus‑induced hyperpermeability of human endothelial cells". Experimental and Therapeutic Medicine 16.2 (2018): 1295-1303.
Chicago
Tian, Y., He, Y., Zhang, L., Zhang, J., Xu, L., Ma, Y., Xu, X., Wei, L."Role of vasodilator‑stimulated phosphoprotein in human cytomegalovirus‑induced hyperpermeability of human endothelial cells". Experimental and Therapeutic Medicine 16, no. 2 (2018): 1295-1303. https://doi.org/10.3892/etm.2018.6332
Copy and paste a formatted citation
x
Spandidos Publications style
Tian Y, He Y, Zhang L, Zhang J, Xu L, Ma Y, Xu X and Wei L: Role of vasodilator‑stimulated phosphoprotein in human cytomegalovirus‑induced hyperpermeability of human endothelial cells. Exp Ther Med 16: 1295-1303, 2018.
APA
Tian, Y., He, Y., Zhang, L., Zhang, J., Xu, L., Ma, Y. ... Wei, L. (2018). Role of vasodilator‑stimulated phosphoprotein in human cytomegalovirus‑induced hyperpermeability of human endothelial cells. Experimental and Therapeutic Medicine, 16, 1295-1303. https://doi.org/10.3892/etm.2018.6332
MLA
Tian, Y., He, Y., Zhang, L., Zhang, J., Xu, L., Ma, Y., Xu, X., Wei, L."Role of vasodilator‑stimulated phosphoprotein in human cytomegalovirus‑induced hyperpermeability of human endothelial cells". Experimental and Therapeutic Medicine 16.2 (2018): 1295-1303.
Chicago
Tian, Y., He, Y., Zhang, L., Zhang, J., Xu, L., Ma, Y., Xu, X., Wei, L."Role of vasodilator‑stimulated phosphoprotein in human cytomegalovirus‑induced hyperpermeability of human endothelial cells". Experimental and Therapeutic Medicine 16, no. 2 (2018): 1295-1303. https://doi.org/10.3892/etm.2018.6332
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