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Natural killer cells inhibit metastasis of ovarian carcinoma cells and show therapeutic effects in a murine model of ovarian cancer

  • Authors:
    • Yanming Sun
    • Zhitao Yao
    • Zhihua Zhao
    • Haifeng Xiao
    • Mengting Xia
    • Xiaojun Zhu
    • Xuelu Jiang
    • Chuntao Sun
  • View Affiliations / Copyright

    Affiliations: Department of Interventional Radiology, Weifang People's Hospital, Weifang, Shandong 261041, P.R. China, Department of Nuclear Medicine, Weifang People's Hospital, Weifang, Shandong 261041, P.R. China, Department of Medical Oncology, Weifang People's Hospital, Weifang, Shandong 261041, P.R. China, Department of Gynecology, Zhejiang Provincial Hospital of Chinese Medicine, Hangzhou, Zhejiang 310006, P.R. China, Department of Gynecology, Women's Hospital, School of Medicine, Zhejiang University, Hangzhou, Zhejiang 310006, P.R. China
    Copyright: © Sun et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 1071-1078
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    Published online on: June 22, 2018
       https://doi.org/10.3892/etm.2018.6342
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Abstract

Ovarian cancer has the highest mortality rate and is the most common of all gynecologic malignancies. Novel treatments for ovarian cancer are urgently required to improve outcomes and the overall survival of patients. The present study investigated whether immunotherapy with natural killer (NK) cells affected the survival of mice with ovarian cancer. Results analysis identified adjunctive NK cells as a potential therapeutic method in ovarian cancer. Patient‑derived ovarian cells were isolated, cultured and subsequently injected subcutaneously into immune deficient BALB/c‑nude mice. Human NK cells were isolated from peripheral blood mononuclear cells and cultured for expansion in vitro. The present results demonstrated that ovarian cells in BALB/c‑nude mice did not induce spontaneous ovarian cancer cell metastasis in the NK‑treated group. In addition, NK cells activated immune cells in the immune system, which resulted in inhibition of ovarian tumor growth in vitro and in a murine xenograft model of ovarian cancer. The data also indicated that cytotoxic activity of NK cells prevented migration and invasion of ovarian cancer cells, which contributed to prevention of systemic metastasis and suggested that NK cells could be effective cells for therapy against ovarian cancer. Furthermore, NK cells induced apoptosis and increased the number of cluster of differentiation (CD)4+, CD8+ as well as cytotoxic T lymphocyte responses by intravenous injection in a murine xenograft model of ovarian cancer. These results suggested that NK cells inhibited the systemic metastasis for ovarian cancer cells. In conclusion, the present study suggested that NK cell immunotherapy inhibited systemic metastasis of ovarian cancer cells and improved the survival rate of mice. Sufficient supplementation of NK cells may serve as a promising immunotherapeutic strategy for ovarian cancer.
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Copy and paste a formatted citation
Spandidos Publications style
Sun Y, Yao Z, Zhao Z, Xiao H, Xia M, Zhu X, Jiang X and Sun C: Natural killer cells inhibit metastasis of ovarian carcinoma cells and show therapeutic effects in a murine model of ovarian cancer. Exp Ther Med 16: 1071-1078, 2018.
APA
Sun, Y., Yao, Z., Zhao, Z., Xiao, H., Xia, M., Zhu, X. ... Sun, C. (2018). Natural killer cells inhibit metastasis of ovarian carcinoma cells and show therapeutic effects in a murine model of ovarian cancer. Experimental and Therapeutic Medicine, 16, 1071-1078. https://doi.org/10.3892/etm.2018.6342
MLA
Sun, Y., Yao, Z., Zhao, Z., Xiao, H., Xia, M., Zhu, X., Jiang, X., Sun, C."Natural killer cells inhibit metastasis of ovarian carcinoma cells and show therapeutic effects in a murine model of ovarian cancer". Experimental and Therapeutic Medicine 16.2 (2018): 1071-1078.
Chicago
Sun, Y., Yao, Z., Zhao, Z., Xiao, H., Xia, M., Zhu, X., Jiang, X., Sun, C."Natural killer cells inhibit metastasis of ovarian carcinoma cells and show therapeutic effects in a murine model of ovarian cancer". Experimental and Therapeutic Medicine 16, no. 2 (2018): 1071-1078. https://doi.org/10.3892/etm.2018.6342
Copy and paste a formatted citation
x
Spandidos Publications style
Sun Y, Yao Z, Zhao Z, Xiao H, Xia M, Zhu X, Jiang X and Sun C: Natural killer cells inhibit metastasis of ovarian carcinoma cells and show therapeutic effects in a murine model of ovarian cancer. Exp Ther Med 16: 1071-1078, 2018.
APA
Sun, Y., Yao, Z., Zhao, Z., Xiao, H., Xia, M., Zhu, X. ... Sun, C. (2018). Natural killer cells inhibit metastasis of ovarian carcinoma cells and show therapeutic effects in a murine model of ovarian cancer. Experimental and Therapeutic Medicine, 16, 1071-1078. https://doi.org/10.3892/etm.2018.6342
MLA
Sun, Y., Yao, Z., Zhao, Z., Xiao, H., Xia, M., Zhu, X., Jiang, X., Sun, C."Natural killer cells inhibit metastasis of ovarian carcinoma cells and show therapeutic effects in a murine model of ovarian cancer". Experimental and Therapeutic Medicine 16.2 (2018): 1071-1078.
Chicago
Sun, Y., Yao, Z., Zhao, Z., Xiao, H., Xia, M., Zhu, X., Jiang, X., Sun, C."Natural killer cells inhibit metastasis of ovarian carcinoma cells and show therapeutic effects in a murine model of ovarian cancer". Experimental and Therapeutic Medicine 16, no. 2 (2018): 1071-1078. https://doi.org/10.3892/etm.2018.6342
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