Expression and significance of autophagy genes LC3, Beclin1 and MMP-2 in endometriosis

Sui,Xiaoyu; Li,Yadong; Sun,Yurong; Li,Chunyan; Li,Xiulan; Zhang,Guiyu

doi:10.3892/etm.2018.6362

Expression and significance of autophagy genes LC3, Beclin1 and MMP-2 in endometriosis

Authors:
- Xiaoyu Sui
- Yadong Li
- Yurong Sun
- Chunyan Li
- Xiulan Li
- Guiyu Zhang
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Affiliations: Department of Obstetrics and Gynecology, The First Affiliated Hospital of Qiqihar Medical University, Qiqihar, Heilongjiang 161041, P.R. China, Clinical Laboratory, The Third Affiliated Hospital of Qiqihar Medical University, Qiqihar, Heilongjiang 161006, P.R. China, Clinicopathological Diagnosis Center, Qiqihar Medical University, Qiqihar, Heilongjiang 161000, P.R. China, Department of Obstetrics and Gynecology, Qilu Hospital of Shandong University, Jinan, Shandong 250012, P.R. China
Published online on: June 27, 2018 https://doi.org/10.3892/etm.2018.6362
Pages: 1958-1962
Copyright: © Sui et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Expression of autophagy-related proteins, microtubule-associated protein light chain 3 (LC3) and Beclin1, and matrix metalloproteinase-2 (MMP-2) was investigated in serum and peritoneal fluid of patients with endometriosis (EM). The messenger ribonucleic acid (mRNA) expression levels of MMP-2, LC3 and Beclin1 in endometrial tissues of EM patients and correlation of these genes with EM and their significance were evaluated. The serum, peritoneal fluid and endometrial tissues of 84 patients treated in The First Affiliated Hospital of Qiqihar Medical University (Qiqihar, China) from March 2016 to March 2017 were collected. The serum, peritoneal fluid and endometrial tissues of 42 EM patients were used as the experimental group, while those of 42 non-EM patients were used as the control group. The levels of LC3, Beclin1 and MMP-2 in serum and peritoneal fluid of EM patients and non-EM patients were quantitatively detected via enzyme-linked immunosorbent assay (ELISA), followed by comparative analysis based on data in both groups. In addition, mRNA expression of LC3, Beclin1 and MMP-2 in the endometrium in both groups were detected via reverse transcription-quantitative polymerase chain reaction (RT-qPCR), and differences in expression of these genes between the groups were analyzed and evaluated. Correlation of LC3, Beclin1 and MMP-2 with EM was explored. Results of ELISA showed that levels of LC3 and Beclin1 in the EM group were significantly lower than those in the control group, while levels of MMP-2 in serum and peritoneal fluid of the EM group were significantly higher than those in the control group (P<0.05). Results of RT-qPCR revealed that mRNA expression of LC3 and Beclin1 in the endometrium of patients in the EM group were obviously decreased compared with those in the control group, while the expression of MMP-2 was high, and differences in expression were statistically significant (P<0.05). The expression of MMP-2 is high, and expression of LC3 and Beclin1 is low in serum, peritoneal fluid and endometrium of EM patients, and investigating the expression of MMP-2, LC3 and Beclin1 in EM is helpful to further clarify the pathogenesis of EM, and guide the clinical diagnosis and treatment.

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1	Hudelist G, Tammaa A, Aas-Eng MK, Kirchner L, Fritzer N, Nemeth Z and Lamche M: Outcome of sonography-based minimally invasive surgery for deep infiltrating endometriosis of the ureter and urinary bladder - a retrospective cohort study. Acta Obstet Gynecol Scand. 97:277–284. 2018. View Article : Google Scholar : PubMed/NCBI
2	Raad J, Sonigo C, Tran C, Sifer C, Durnerin IC and Grynberg M: Oocyte vitrification for preserving fertility in patients with endometriosis: First observational cohort study… and many unresolved questions. Letter to the Editor. Eur J Obstet Gynecol Reprod Biol. 220:140–141. 2018. View Article : Google Scholar : PubMed/NCBI
3	Yang HL, Mei J, Chang KK, Zhou WJ, Huang LQ and Li MQ: Autophagy in endometriosis. Am J Transl Res. 9:4707–4725. 2017.PubMed/NCBI
4	Orazov MR, Radzinskiy VY, Khamoshina MB, Nosenko EN, Tokaeva ES, Barsegyan LK and Zakirova YR: Histamine metabolism disorder in pathogenesis of chronic pelvic pain in patients with external genital endometriosis. Patol Fiziol Eksp Ter. 61:56–60. 2017.(In Russian). PubMed/NCBI
5	Shukla PS and Schwartz C: Extrauterine, extragenital endometrial stromal sarcoma in the absence of endometriosis: A rare entity. Int J Gynecol Pathol. Nov 28–2017.(Epub ahead of print). View Article : Google Scholar : PubMed/NCBI
6	Nada AM, El-Noury A, Al-Inany H, Bibars M, Taha T, Salama S, Hassan F and Zein E: Effect of laser-assisted zona thinning, during assisted reproduction, on pregnancy outcome in women with endometriosis: Randomized controlled trial. Arch Gynecol Obstet. 297:521–528. 2018. View Article : Google Scholar : PubMed/NCBI
7	Mohagheghi S, Khodadadi I, Karami M, Amiri I and Tavilani H: Gene polymorphism of matrix metalloproteinase 9 in asthenozoospermic male subjects. Int J Fertil Steril. 11:247–252. 2018.PubMed/NCBI
8	Wu RF, Huang ZX, Ran J, Dai SJ, Lin DC, Ng TW, Chen QX and Chen QH: Lipoxin A4 suppresses estrogen-induced epithelial-mesenchymal transition via ALXR-dependent manner in endometriosis. Reprod Sci. 25:566–578. 2018. View Article : Google Scholar : PubMed/NCBI
9	Livak KJ and Schmittgen TD: Analysis of relative geneexpression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method. METHODS. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI
10	Park S, Lim W, Bazer FW and Song G: Apigenin induces ROS-dependent apoptosis and ER stress in human endometriosis cells. J Cell Physiol. 233:3055–3065. 2018. View Article : Google Scholar : PubMed/NCBI
11	Jana S, Chatterjee K, Ray AK, DasMahapatra P and Swarnakar S: Regulation of matrix metalloproteinase-2 activity by COX-2-PGE2-pAKT axis promotes angiogenesis in endometriosis. PLoS One. 11:e01635402016. View Article : Google Scholar : PubMed/NCBI
12	Yang WW, Hong L, Xu XX, Wang Q, Huang JL and Jiang L: Regulation of miR-33b on endometriosis and expression of related factors. Eur Rev Med Pharmacol Sci. 21:2027–2033. 2017.PubMed/NCBI
13	Chen YS, Langhammer T, Westhofen M and Lorenzen J: Relationship between matrix metalloproteinases MMP-2, MMP-9, tissue inhibitor of matrix metalloproteinases-1 and IL-5, IL-8 in nasal polyps. Allergy. 62:66–72. 2007. View Article : Google Scholar : PubMed/NCBI
14	Yang H, Liu J, Fan Y, Guo Q, Ge L, Yu N, Zheng X, Dou Y and Zheng S: Associations between various possible promoter polymorphisms of MMPs genes and endometriosis risk: A meta-analysis. Eur J Obstet Gynecol Reprod Biol. 205:174–188. 2016. View Article : Google Scholar : PubMed/NCBI
15	Katagiri H, Nakayama K, Razia S, Nakamura K, Sato E, Ishibashi T, Ishikawa M, Iida K, Ishikawa N, Otsuki Y, et al: Loss of autophagy-related protein Beclin 1 may define poor prognosis in ovarian clear cell carcinomas. Int J Oncol. 47:2037–2044. 2015. View Article : Google Scholar : PubMed/NCBI
16	Shi J, Shi S, Wu B, Zhang J, Li Y, Wu X, Zhang J, Wang K, Zhao B, Cai W, et al: Autophagy protein LC3 regulates the fibrosis of hypertrophic scar by controlling Bcl-xL in dermal fibroblasts. Oncotarget. 8:93757–93770. 2017.PubMed/NCBI
17	Zhu ML, Lu JX, Pan GP, Ping S, Zhao FR, Qi HT, Yu HY, Jian X, Wan GR and Li P: Traditional Chinese medicine Ka-Sai-Ping suppresses the growths of gastric cancers via induction of autophagy. Oncotarget. 8:95075–95082. 2017.PubMed/NCBI
18	Machado DE, Rodrigues-Baptista KC, Alessandra-Perini J, de Moura Soares R, Santos TA, Pereira KG, da Silva Marinho Y, Souza PJ, Nasciutti LE and Perini JA: Euterpe oleracea extract (Açaí) is a promising novel pharmacological therapeutic treatment for experimental endometriosis. PLoS One. 11:e01660592016. View Article : Google Scholar : PubMed/NCBI
19	Ye H, He Y, Wang J, Song T, Lan Z, Zhao Y and Xi M: Effect of matrix metalloproteinase promoter polymorphisms on endometriosis and adenomyosis risk: Evidence from a meta-analysis. J Genet. 95:611–619. 2016. View Article : Google Scholar : PubMed/NCBI
20	Boone BA, Zeh RH and Bahary N: Autophagy inhibition in pancreatic adenocarcinoma. Clin Colorectal Cancer. 17:25–31. 2017. View Article : Google Scholar : PubMed/NCBI
21	Lin SC, Chu PY, Liao WT, Wu MY, Tsui KH, Lin LT, Huang CH, Chen LL and Li CJ: Glycyrrhizic acid induces human MDA-MB-231 breast cancer cell death and autophagy via the ROS-mitochondrial pathway. Oncol Rep. 39:703–710. 2018.PubMed/NCBI
22	Fan T, Pi H, Li M, Ren Z, He Z, Zhu F, Tian L, Tu M, Xie J, Liu M, et al: Inhibiting MT2-TFE3-dependent autophagy enhances melatonin-induced apoptosis in tongue squamous cell carcinoma. J Pineal Res. 64:642018. View Article : Google Scholar
23	Rubio MF, Lira MC, Rosa FD, Sambresqui AD, Salazar Güemes MC and Costas MA: RAC3 influences the chemoresistance of colon cancer cells through autophagy and apoptosis inhibition. Cancer Cell Int. 17:1112017. View Article : Google Scholar : PubMed/NCBI
24	Peng Q, Qin J, Zhang Y, Cheng X, Wang X, Lu W, Xie X and Zhang S: Autophagy maintains the stemness of ovarian cancer stem cells by FOXA2. J Exp Clin Cancer Res. 36:1712017. View Article : Google Scholar : PubMed/NCBI
25	Sultana S, Dey R and Bishayi B: Dual neutralization of TNFR-2 and MMP-2 regulates the severity of S. aureus induced septic arthritis correlating alteration in the level of interferon gamma and interleukin-10 in terms of TNFR2 blocking. Immunol Res. 66:97–119. 2018. View Article : Google Scholar : PubMed/NCBI
26	Jana S, Rudra DS, Paul S and Snehasikta S: Curcumin delays endometriosis development by inhibiting MMP-2 activity. Indian J Biochem Biophys. 49:342–348. 2012.PubMed/NCBI
27	Pourjafar M, Saidijam M, Etemadi K and Najafi R: All-trans retinoic acid enhances in vitro mesenchymal stem cells migration by targeting matrix metalloproteinases 2 and 9. Biotechnol Lett. 39:1263–1268. 2017. View Article : Google Scholar : PubMed/NCBI