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Article

MicroRNA‑21 promotes the proliferation, migration and invasion of non‑small cell lung cancer A549 cells by regulating autophagy activity via AMPK/ULK1 signaling pathway

  • Authors:
    • Shuping Li
    • Xiaofei Zeng
    • Ruidong Ma
    • Li Wang
  • View Affiliations / Copyright

    Affiliations: Department of Cardiothoracic Surgery, The First Affiliated Hospital of Chengdu Medical College, Chengdu, Sichuan 610500, P.R. China, Department of Anesthesiology, The First Affiliated Hospital of Chengdu Medical College, Chengdu, Sichuan 610500, P.R. China
  • Pages: 2038-2045
    |
    Published online on: June 29, 2018
       https://doi.org/10.3892/etm.2018.6370
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Abstract

The present study investigated the expression of microRNA (miR)‑21 in non‑small cell lung cancer (NSCLC) tissues, its biological functions and mechanism of autophagy regulation. A total of 46 patients with NSCLC were enrolled in the present study. To measure the expression of miR‑21, reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR) was employed. NSCLC A549 cells were transfected with miR‑negative control (NC), miR‑21 mimics or inhibitor. The CCK‑8 assay was used to investigate the proliferation of A549 cells. To study migration and invasion abilities of A549 cells, The Transwell assay was performed. In addition, to determine the expression levels of ULK1, LC3B, AMPKα, p‑AMPKα and p62 proteins, western blotting was conducted and laser confocal microscopy was performed to observe the formation of autophagosomes in A549 cells. To explore whether miR‑21 regulates the biological functions of A549 cells via autophagy, an autophagy inhibitor, 3‑MA, or agonist, rapamycin, were used in a rescue assay. Results indicated that miR‑21 expression in NSCLC tissues was enhanced, and closely correlated with the occurrence and development of NSCLC. In vitro experiments showed that miR‑21 mimics promoted the proliferation, migration and invasion of A549 cells, while miR‑21 inhibitor inhibited these biological functions. Western blotting indicated that miR‑21 upregulated autophagy marker LC3BⅡ protein, but downregulated p62 protein. Laser confocal microscopy showed that miR‑21 activated autophagy of A549. Rescue experiments indicated that autophagy reversed the effect of miR‑21 on the proliferation, migration and invasion of A549 cells. Western blotting data suggested that autophagy‑related AMPK/ULK1 signaling pathway was activated by miR‑21, and interference or overexpression of ULK1 reversed the biological functions of miR‑21. The present study demonstrated that miR‑21 expression in NSCLC tissues was upregulated and positively correlated with lymphatic metastasis and clinical staging. In addition, miR‑21 regulated autophagy activity of NSCLC A549 cells via AMPK/ULK1 signaling pathway, and promoted the proliferation, migration and invasion of NSCLC A549 cells.
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1 

Schneider G, Sellers ZP, Bujko K, Kakar SS, Kucia M and Ratajczak MZ: Novel pleiotropic effects of bioactive phospholipids in human lung cancer metastasis. Oncotarget. 8:58247–58263. 2017. View Article : Google Scholar : PubMed/NCBI

2 

Choi EJ, Jung BJ, Lee SH, Yoo HS, Shin EA, Ko HJ, Chang S, Kim SY and Jeon SM: A clinical drug library screen identifies clobetasol propionate as an NRF2 inhibitor with potential therapeutic efficacy in KEAP1 mutant lung cancer. Oncogene. 36:5285–5295. 2017. View Article : Google Scholar : PubMed/NCBI

3 

Tian Y, Liu Q and Wu K, Chu Q, Chen Y and Wu K: Meta-analysis comparing the efficacy of nedaplatin-based regimens between squamous cell and non-squamous cell lung cancers. Oncotarget. 8:62330–62338. 2017. View Article : Google Scholar : PubMed/NCBI

4 

Tong YH, Zhang B, Yan YY, Fan Y, Yu JW, Kong SS, Zhang D, Fang L, Su D and Lin NM: Dual-negative expression of Nrf2 and NQO1 predicts superior outcomes in patients with non-small cell lung cancer. Oncotarget. 8:45750–45758. 2017. View Article : Google Scholar : PubMed/NCBI

5 

Xu W, Wu B, Fu L, Chen J, Wang Z, Huang F, Chen J, Zhang M, Zhang Z, Lin J, et al: Comparison of three different methods for the detection of circulating tumor cells in mice with lung metastasis. Oncol Rep. 37:3219–3226. 2017. View Article : Google Scholar : PubMed/NCBI

6 

Zhang Y, Sui J, Shen X, Li C, Yao W, Hong W, Peng H, Pu Y, Yin L and Liang G: Differential expression profiles of microRNAs as potential biomarkers for the early diagnosis of lung cancer. Oncol Rep. 37:3543–3553. 2017. View Article : Google Scholar : PubMed/NCBI

7 

Icli B and Feinberg MW: MicroRNAs in dysfunctional adipose tissue: Cardiovascular implications. Cardiovasc Res. 113:1024–1034. 2017. View Article : Google Scholar : PubMed/NCBI

8 

Avellini C, Licini C, Lazzarini R, Gesuita R, Guerra E, Tossetta G, Castellucci C, Giannubilo SR, Procopio A, Alberti S, et al: The trophoblast cell surface antigen 2 and miR-125b axis in urothelial bladder cancer. Oncotarget. 8:58642–58653. 2017. View Article : Google Scholar : PubMed/NCBI

9 

Xiang Y, Liao XH, Yu CX, Yao A, Qin H, Li JP, Hu P, Li H, Guo W, Gu CJ and Zhang TC: MiR-93-5p inhibits the EMT of breast cancer cells via targeting MKL-1 and STAT3. Exp Cell Res. 357:135–144. 2017. View Article : Google Scholar : PubMed/NCBI

10 

Xu X, Jin S, Ma Y, Fan Z, Yan Z, Li W, Song Q, You W, Lyu Z, Song Y, et al: miR-30a-5p enhances paclitaxel sensitivity in non-small cell lung cancer through targeting BCL-2 expression. J Mol Med (Berl). 95:861–871. 2017. View Article : Google Scholar : PubMed/NCBI

11 

Hou XW, Sun X, Yu Y, Zhao HM, Yang ZJ, Wang X and Cao XC: miR-361-5p suppresses lung cancer cell lines progression by targeting FOXM1. Neoplasma. 64:526–534. 2017. View Article : Google Scholar : PubMed/NCBI

12 

Sosa Arias LA, Orduz Cuspoca AF and Bernal Gómez BM: Deregulation of microRNAs in gastric cancer: Up regulation by miR-21 and miR-106. Rev Gastroenterol Peru. 37:65–70. 2017.(In Spanish). PubMed/NCBI

13 

Markou A, Zavridou M and Lianidou ES: miRNA-21 as a novel therapeutic target in lung cancer. Lung Cancer (Auckl). 7:19–27. 2016.PubMed/NCBI

14 

Dong J, Zhang Z, Gu T, Xu SF, Dong LX, Li X, Fu BH and Fu ZZ: The role of microRNA-21 in predicting brain metastases from non-small cell lung cancer. Onco Targets Ther. 10:185–194. 2016. View Article : Google Scholar : PubMed/NCBI

15 

Horibe A, Eid N, Ito Y, Hamaoka H, Tanaka Y and Kondo Y: Upregulated autophagy in sertoli cells of ethanol-treated rats is associated with induction of inducible nitric oxide synthase (iNOS), androgen receptor suppression and germ cell apoptosis. Int J Mol Sci. 18:pii: E1061. 2017.PubMed/NCBI

16 

Krause GC, Lima KG, Dias HB, da Silva EFG, Haute GV, Basso BS, Gassen RB, Marczak ES, Nunes RSB and de Oliveira JR: Liraglutide, a glucagon-like peptide-1 analog, induce autophagy and senescence in HepG2 cells. Eur J Pharmacol. 809:32–41. 2017. View Article : Google Scholar : PubMed/NCBI

17 

Zheng Z, Xu L, Zhang S, Li W, Tou F, He Q, Rao J and Shen Q: Peiminine inhibits colorectal cancer cell proliferation by inducing apoptosis and autophagy and modulating key metabolic pathways. Oncotarget. 8:47619–47631. 2017.PubMed/NCBI

18 

Dai R, Zhang S, Duan W, Wei R, Chen H, Cai W, Yang L and Wang Q: Enhanced autophagy contributes to protective effects of GM1 Ganglioside Against Aβ1-42-induced neurotoxicity and cognitive deficits. Neurochem Res. 42:2417–2426. 2017. View Article : Google Scholar : PubMed/NCBI

19 

Lin Y, Zhao J, Wang H, Cao J and Nie Y: miR-181a modulates proliferation, migration and autophagy in AGS gastric cancer cells and downregulates MTMR3. Mol Med Rep. 15:2451–2456. 2017. View Article : Google Scholar : PubMed/NCBI

20 

Wei R, Cao G, Deng Z, Su J and Cai L: miR-140-5p attenuates chemotherapeutic drug-induced cell death by regulating autophagy through inositol 1,4,5-trisphosphate kinase 2 (IP3k2) in human osteosarcoma cells. Biosci Rep. 36:pii: e00392. 1016.

21 

Jiang LP, He CY and Zhu ZT: Role of microRNA-21 in radiosensitivity in non-small cell lung cancer cells by targeting PDCD4 gene. Oncotarget. 8:23675–23689. 2017.PubMed/NCBI

22 

Yuan C, Zheng Y, Zhang B, Shao L, Liu Y, Tian T, Gu X, Li X and Fan K: Thymosin α1 promotes the activation of myeloid-derived suppressor cells in a Lewis lung cancer model by upregulating Arginase 1. Biochem Biophys Res Commun. 464:249–255. 2015. View Article : Google Scholar : PubMed/NCBI

23 

Puspawati NLPD, Sitorus R and Herawati T: Hand-held Fan airflow stimulation relieves dyspnea in lung cancer patients. Asia Pac J Oncol Nurs. 4:162–167. 2017. View Article : Google Scholar : PubMed/NCBI

24 

Deng ZM, Liu L, Qiu WH, Zhang YQ, Zhong HY, Liao P and Wu YH: Analysis of genomic variation in lung adenocarcinoma patients revealed the critical role of PI3K complex. PeerJ. 5:e32162017. View Article : Google Scholar : PubMed/NCBI

25 

Duan B, Guo T, Sun H, Cai R, Rui Q and Xi Z: miR-205 as a biological marker in non-small cell lung cancer. Biomed Pharmacother. 91:823–830. 2017. View Article : Google Scholar : PubMed/NCBI

26 

Zhang L, Yao J, Li W and Zhang C: Micro-RNA-21 regulates cancer-associated fibroblast-mediated frug resistance in pancreatic cancer. Oncol Res. May 5–2017.(Epub ahead of print).

27 

Feng Y, Zou W, Hu C, Li G, Zhou S, He Y, Ma F, Deng C and Sun L: Modulation of CASC2/miR-21/PTEN pathway sensitizes cervical cancer to cisplatin. Arch Biochem Biophys. 623–624:20–30. 2017. View Article : Google Scholar

28 

Shao YY, Zhang TL, Wu LX, Zou HC, Li S, Huang J and Zhou HH: AKT axis, miR-21, and RECK play pivotal roles in dihydroartemisinin killing malignant glioma cells. Int J Mol Sci. 18:pii: E350. 2017. View Article : Google Scholar

29 

Pursiheimo JP, Rantanen K, Heikkinen PT, Johansen T and Jaakkola PM: Hypoxia-activated autophagy accelerates degradation of SQSTM1/p62. Oncogene. 28:334–344. 2009. View Article : Google Scholar : PubMed/NCBI

30 

Moon HS, Kim B, Gwak H, Suh DH and Song YS: Autophagy and protein kinase RNA-like endoplasmic reticulum kinase (PERK)/eukaryotic initiation factor 2 alpha kinase (eIF2α) pathway protect ovarian cancer cells from metformin-induced apoptosis. Mol Carcinog. 55:346–356. 2016. View Article : Google Scholar : PubMed/NCBI

31 

Hong-Brown LQ, Brown CR, Navaratnarajah M and Lang CH: FoxO1-AMPK-ULK1 regulates ethanol-induced autophagy in muscle by enhanced ATG14 association with the BECN1-PIK3C3 complex. Alcohol Clin Exp Res. 41:895–910. 2017. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Li S, Zeng X, Ma R and Wang L: MicroRNA‑21 promotes the proliferation, migration and invasion of non‑small cell lung cancer A549 cells by regulating autophagy activity via AMPK/ULK1 signaling pathway. Exp Ther Med 16: 2038-2045, 2018.
APA
Li, S., Zeng, X., Ma, R., & Wang, L. (2018). MicroRNA‑21 promotes the proliferation, migration and invasion of non‑small cell lung cancer A549 cells by regulating autophagy activity via AMPK/ULK1 signaling pathway. Experimental and Therapeutic Medicine, 16, 2038-2045. https://doi.org/10.3892/etm.2018.6370
MLA
Li, S., Zeng, X., Ma, R., Wang, L."MicroRNA‑21 promotes the proliferation, migration and invasion of non‑small cell lung cancer A549 cells by regulating autophagy activity via AMPK/ULK1 signaling pathway". Experimental and Therapeutic Medicine 16.3 (2018): 2038-2045.
Chicago
Li, S., Zeng, X., Ma, R., Wang, L."MicroRNA‑21 promotes the proliferation, migration and invasion of non‑small cell lung cancer A549 cells by regulating autophagy activity via AMPK/ULK1 signaling pathway". Experimental and Therapeutic Medicine 16, no. 3 (2018): 2038-2045. https://doi.org/10.3892/etm.2018.6370
Copy and paste a formatted citation
x
Spandidos Publications style
Li S, Zeng X, Ma R and Wang L: MicroRNA‑21 promotes the proliferation, migration and invasion of non‑small cell lung cancer A549 cells by regulating autophagy activity via AMPK/ULK1 signaling pathway. Exp Ther Med 16: 2038-2045, 2018.
APA
Li, S., Zeng, X., Ma, R., & Wang, L. (2018). MicroRNA‑21 promotes the proliferation, migration and invasion of non‑small cell lung cancer A549 cells by regulating autophagy activity via AMPK/ULK1 signaling pathway. Experimental and Therapeutic Medicine, 16, 2038-2045. https://doi.org/10.3892/etm.2018.6370
MLA
Li, S., Zeng, X., Ma, R., Wang, L."MicroRNA‑21 promotes the proliferation, migration and invasion of non‑small cell lung cancer A549 cells by regulating autophagy activity via AMPK/ULK1 signaling pathway". Experimental and Therapeutic Medicine 16.3 (2018): 2038-2045.
Chicago
Li, S., Zeng, X., Ma, R., Wang, L."MicroRNA‑21 promotes the proliferation, migration and invasion of non‑small cell lung cancer A549 cells by regulating autophagy activity via AMPK/ULK1 signaling pathway". Experimental and Therapeutic Medicine 16, no. 3 (2018): 2038-2045. https://doi.org/10.3892/etm.2018.6370
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