Nerve growth factor and substance P may be involved in moist exposed burn ointment‑mediated chronic refractory wound healing

Fu,Huang‑De; Wang,Shu; Ge,Bin; Li,Li‑Qing; Zeng,Hong‑Meng; Shu,Qing‑Feng; Zhou,Yang

doi:10.3892/etm.2018.6390

September-2018 Volume 16 Issue 3

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September-2018 Volume 16 Issue 3

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Article Open Access

Nerve growth factor and substance P may be involved in moist exposed burn ointment‑mediated chronic refractory wound healing

Authors:
- Huang‑De Fu
- Shu Wang
- Bin Ge
- Li‑Qing Li
- Hong‑Meng Zeng
- Qing‑Feng Shu
- Yang Zhou
View Affiliations / Copyright

Affiliations: The First Affiliated Hospital of Jinan University, Guangzhou, Guangdong 510632, P.R. China, Graduate School of Youjiang Medical University for Nationalities, Baise, Guangxi 533000, P.R. China

Copyright: © Fu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
Pages: 1987-1993
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Published online on: July 2, 2018

https://doi.org/10.3892/etm.2018.6390
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Abstract

Moist exposed burn ointment (MEBO) is becoming increasingly popular in China as it shortens wound‑healing time and reduces scar formation. However, its exact mechanism in mediating the wound‑healing process is not yet clear. In the present study a total of 90 healthy adult male Wistar rats of specific-pathogen-free grade were divided equally into a control group, wound group, MEBO group, recombinant bovine basic fibroblast growth factor (rb‑bFGF) group and sham operation group. Wound healing was observed from the extracted granulation tissues and recorded at three time points on 3, 7 and 14 days. Different levels of tumor necrosis factor α (TNF‑α) and interleukin‑6 (IL‑6) in tissue homogenate were detected using ELISA. Western blot analysis and quantitative PCR were used to detect the expression of nerve growth factor (NGF), substance P (SP) as well as tyrosine kinase A (TrkA) receptor protein and the corresponding mRNA levels in granulation tissue. It was observed that the wound healing progressed faster in the MEBO and rb‑bFGF groups compared with the wound group (P<0.01). TNF‑α and IL‑6 had an upward‑downward trend at three time points, with the wound group demonstrating the most obvious increase (P<0.01). NGF and SP mRNA and protein levels in granulation tissue in MEBO, rb‑bFGF and sham operation groups reached their highest levels on day 7 and then decreased on day 14. The expression level of TrkA was also measured simultaneously and its expression pattern was similar to that of NGF and SP. These results suggested that MEBO may promote nerve repair and accelerate wound healing through mediating the expression levels of NGF and SP, as well as TrkA.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

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Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Nerve growth factor and substance P may be involved in moist exposed burn ointment‑mediated chronic refractory wound healing

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