Open Access

Preliminary study on the effect of brazilin on biofilms of Staphylococcus aureus

  • Authors:
    • Dan Peng
    • Anlin Chen
    • Bin Shi
    • Xun Min
    • Tao Zhang
    • Zheling Dong
    • Huan Yang
    • Xianlian Chen
    • Yingbiao Tian
    • Zehui Chen
  • View Affiliations

  • Published online on: July 4, 2018     https://doi.org/10.3892/etm.2018.6403
  • Pages: 2108-2118
  • Copyright: © Peng et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Biofilms significantly enhance antibiotic resistance by inhibiting penetration of antibiotics and are shielded from the immune system via the formation of an extracellular polymeric matrix. Innovative and novel approaches are required for the inhibition of biofilm formation and treatment of biofilm‑associated infectious diseases. In the current study, a biofilm model of Staphylococcus aureus was established in vitro to explore inhibitory effects of brazilin (BN) on biofilm formation and to evaluate damaging effects of BN in the presence and absence of vancomycin (VCM) on the biofilm. Antibiofilm‑infection mechanisms of BN were observed. In these experiments, the clinical strain of S. aureus C‑4‑4 was isolated for biofilm formation. Crystal violet staining and fluorescence microscopy revealed that BN inhibited biofilm formation in vitro and the best effect was observed with two times the minimum inhibitory concentration of BN following 48 h incubation. Additionally, the results demonstrated that BN in combination with VCM enhanced the damage to biofilms, whereas VCM alone did not. The results of the reverse transcription‑quantitative polymerase chain reaction analyses demonstrated that BN downregulated gene expression of intercellular adhesion (ica)A and upregulated icaR and the quorum‑sensing (QS) system regulator accessory gene regulator A. In summary, BN inhibited S. aureus biofilm formation and destroyed biofilms, while simultaneously increasing permeability to VCM. BN was able to reduce production of the extracellular polymeric matrix and inhibited the QS system. These results support the use of BN as a novel drug and treatment strategy for S. aureus biofilm‑associated infections.
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September-2018
Volume 16 Issue 3

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Peng D, Chen A, Shi B, Min X, Zhang T, Dong Z, Yang H, Chen X, Tian Y, Chen Z, Chen Z, et al: Preliminary study on the effect of brazilin on biofilms of Staphylococcus aureus. Exp Ther Med 16: 2108-2118, 2018
APA
Peng, D., Chen, A., Shi, B., Min, X., Zhang, T., Dong, Z. ... Chen, Z. (2018). Preliminary study on the effect of brazilin on biofilms of Staphylococcus aureus. Experimental and Therapeutic Medicine, 16, 2108-2118. https://doi.org/10.3892/etm.2018.6403
MLA
Peng, D., Chen, A., Shi, B., Min, X., Zhang, T., Dong, Z., Yang, H., Chen, X., Tian, Y., Chen, Z."Preliminary study on the effect of brazilin on biofilms of Staphylococcus aureus". Experimental and Therapeutic Medicine 16.3 (2018): 2108-2118.
Chicago
Peng, D., Chen, A., Shi, B., Min, X., Zhang, T., Dong, Z., Yang, H., Chen, X., Tian, Y., Chen, Z."Preliminary study on the effect of brazilin on biofilms of Staphylococcus aureus". Experimental and Therapeutic Medicine 16, no. 3 (2018): 2108-2118. https://doi.org/10.3892/etm.2018.6403