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MicroRNA‑101‑3p inhibits proliferation in retinoblastoma cells by targeting EZH2 and HDAC9

  • Authors:
    • Qifang Jin
    • Wenfeng He
    • Leifeng Chen
    • Yang Yang
    • Ke Shi
    • Zhipeng You
  • View Affiliations / Copyright

    Affiliations: Department of Ophthalmology, The Second Affiliated Hospital of Nanchang University, Nanchang, Jiangxi 330006, P.R. China, Jiangxi Key Laboratory of Molecular Medicine, The Second Affiliated Hospital of Nanchang University, Nanchang, Jiangxi 330006, P.R. China
    Copyright: © Jin et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 1663-1670
    |
    Published online on: July 4, 2018
       https://doi.org/10.3892/etm.2018.6405
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Abstract

Retinoblastoma is the most frequent intraocular malignant tumor type to occur in childhood. MicroRNA (miR)‑101‑3p has been reported to function as a tumor suppressor in various types of cancer. However, the biological function and underlying mechanisms of miR‑101‑3p in retinoblastoma are largely unknown. In the present study, it was identified that miR‑101‑3p was downregulated in retinoblastoma. MTT and flow cytometry assays demonstrated that ectopic overexpression of miR‑101‑3p significantly inhibited cell viability and cell cycle progression in WERI‑Rb‑1 and Y79 cells. In vivo mouse experiments further confirmed the anti‑proliferative role of miR‑101‑3p in retinoblastoma. Additionally, predictions with TargetScan software indicated that the 3'‑untranslated regions of enhancer of zeste homolog 2 (EZH2) and histone deacetylase (HDAC9) mRNAs are targeted by miR‑101‑3p. Accordingly, a dual luciferase reporter gene assay demonstrated that miR‑101‑3p directly targeted EZH2 and HDAC9 to suppress the proliferation of retinoblastoma cells. Meanwhile, the restoration of EZH2 or HDAC9 expression countered the anti‑proliferative effect of miR‑101‑3p on WERI‑Rb‑1 and Y79 cells. Collectively, these data highlight the role of miR‑101‑3p in the tumorigenesis of retinoblastoma, and indicate its suitability as a novel therapeutic target.
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Copy and paste a formatted citation
Spandidos Publications style
Jin Q, He W, Chen L, Yang Y, Shi K and You Z: MicroRNA‑101‑3p inhibits proliferation in retinoblastoma cells by targeting EZH2 and HDAC9. Exp Ther Med 16: 1663-1670, 2018.
APA
Jin, Q., He, W., Chen, L., Yang, Y., Shi, K., & You, Z. (2018). MicroRNA‑101‑3p inhibits proliferation in retinoblastoma cells by targeting EZH2 and HDAC9. Experimental and Therapeutic Medicine, 16, 1663-1670. https://doi.org/10.3892/etm.2018.6405
MLA
Jin, Q., He, W., Chen, L., Yang, Y., Shi, K., You, Z."MicroRNA‑101‑3p inhibits proliferation in retinoblastoma cells by targeting EZH2 and HDAC9". Experimental and Therapeutic Medicine 16.3 (2018): 1663-1670.
Chicago
Jin, Q., He, W., Chen, L., Yang, Y., Shi, K., You, Z."MicroRNA‑101‑3p inhibits proliferation in retinoblastoma cells by targeting EZH2 and HDAC9". Experimental and Therapeutic Medicine 16, no. 3 (2018): 1663-1670. https://doi.org/10.3892/etm.2018.6405
Copy and paste a formatted citation
x
Spandidos Publications style
Jin Q, He W, Chen L, Yang Y, Shi K and You Z: MicroRNA‑101‑3p inhibits proliferation in retinoblastoma cells by targeting EZH2 and HDAC9. Exp Ther Med 16: 1663-1670, 2018.
APA
Jin, Q., He, W., Chen, L., Yang, Y., Shi, K., & You, Z. (2018). MicroRNA‑101‑3p inhibits proliferation in retinoblastoma cells by targeting EZH2 and HDAC9. Experimental and Therapeutic Medicine, 16, 1663-1670. https://doi.org/10.3892/etm.2018.6405
MLA
Jin, Q., He, W., Chen, L., Yang, Y., Shi, K., You, Z."MicroRNA‑101‑3p inhibits proliferation in retinoblastoma cells by targeting EZH2 and HDAC9". Experimental and Therapeutic Medicine 16.3 (2018): 1663-1670.
Chicago
Jin, Q., He, W., Chen, L., Yang, Y., Shi, K., You, Z."MicroRNA‑101‑3p inhibits proliferation in retinoblastoma cells by targeting EZH2 and HDAC9". Experimental and Therapeutic Medicine 16, no. 3 (2018): 1663-1670. https://doi.org/10.3892/etm.2018.6405
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