Open Access

Preparation and characterization of norcantharidin liposomes modified with stearyl glycyrrhetinate

  • Authors:
    • Jing Zhu
    • Wei Zhang
    • Dandan Wang
    • Suzhen Li
    • Wei Wu
  • View Affiliations

  • Published online on: July 6, 2018     https://doi.org/10.3892/etm.2018.6416
  • Pages: 1639-1646
  • Copyright: © Zhu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

In the current study, norcantharidin (NCTD)-loaded liposomes (LIPs) modified with stearyl glycyrrhetinate (SG; SG‑NCTD‑LIP) were prepared by the ethanol injection method. To increase the drug encapsulation efficiency (EE), the formulation of NCTD‑LIP was optimized by single factor test and orthogonal design. The release of NCTD in vitro from SG‑NCTD‑LIP was evaluated by equilibrium dialysis. The cytotoxicity of SG‑NCTD‑LIP in HepG2 was investigated by MTT assay. The results revealed that the EE of liposomes was ~27.80%, the average SG‑NCTD‑LIP was 87.5 nm, the in vitro NCTD release from SG‑NCTD‑LIP was delayed compared with NCTD in solution and the drug‑release kinetic followed a first‑order model. MTT assays revealed increased cytotoxicity activity against HepG2 cells for SG‑NCTD‑LIP compared with free NCTD. In conclusion, SG‑NCTD‑LIP prepared in the present study may be a promising liposomal drug delivery system for anticancer drugs in liver‑targeting therapy.

Introduction

Norcantharidin (NCTD; Fig. 1), the demethylated derivative of cantharidin obtained from the dried body of the Chinese blister beetle (Mylabris spp.) (1), has been used as an anticancer drug in China (2,3). NCTD acts by inducing cell death through mechanisms involving the response to DNA damage and apoptosis by activation of the protein kinase C signaling pathway (4). Like sulfonamides for carbonic anhydrase or hydroxamic acids for metalloproteinases, NCTD describes the archetypal small molecule protein phosphatase inhibitor (5) and has been used to inhibit proliferation and metastasis of multiple types of carcinoma (6,7). Previous studies indicated that NCTD has therapeutic value in the treatment of various types of cancer, including liver cancer, when administered orally or intravenously (6,811). However, the application of NCTD is limited by numerous factors, including short half-life, high systemic toxicity, high incidence of adverse effects and poor bioavailability in the physiological environment (12,13). To improve the safety and efficacy of NCTD, NCTD nanoscale drug delivery systems (DDS) have been studied (1416).

Liposomes are spheres with a lipid bilayer shell prepared with a variety of phospholipids, which have been extensively studied since the 1960s (1719). Cholesterol is an additional compound in the liposomal structure that could regulate the fluidity of phospholipid membrane and may control the retention of drugs (20). Therefore, liposomes may be used as nanoscale vehicles for the administration of drugs. With good biocompatibility and low toxicity, liposomes have been revealed to enhance the therapeutic activity of numerous anticancer drugs (2123). However, conventional liposomes have certain disadvantages, including the uptake by the reticuloendothelial system (RES) (24,25), the lack of tumor-specificity and insufficient uptake at tumor sites. Ligand-targeted liposomes, where specific ligands are used to modify liposomes, have demonstrated the potential to increase therapeutic efficacy and reduce adverse effects through the interaction between a specific ligand and the target molecule, and facilitating the receptor mediated endocytosis of liposomes (26).

Glycyrrhetinic acid (GA) may be obtained by hydrolysis of glycyrrhicinate, extracted from the roots of liquorice (Glycyrrhiza glabra) (27). GA possesses many pharmacological and biological relevant activities, including anti-inflammatory, antiallergic, antiulcer, antioxidant, antihepatotoxic, antineoplasmic and antiviral activities (2830). Studies have reported that specific GA binding sites may be located on the cell membrane of hepatocytes (31,32). GA and its derivatives may be used as ligands targeting the liver (33,34). Stearyl glycyrrhetinate (SG; Fig. 2), the stearyl ester of 18-β-glycyrrhetinic acid, has been demonstrated to improve antiviral effects, reduce inflammation and suppress allergies in the pharmaceutical and cosmetic industry (35,36). Compared with GA, SG has an increased compatibility as its hydrophobic tail may be adsorbed into the lipid layer of liposomes while exposing the hydrophilic GA moiety at the surface.

The objective of the current study was to prepare NCTD-loaded liposomes modified with SG (SG-NCTD-LIP) by ethanol injection. Single factor test and orthogonal design were used to optimize the formulation of SG-NCTD-LIP. The characterization of the prepared liposomes included physical morphology, particle size and encapsulation efficiency (EE). Equilibrium dialysis was used to investigate in vitro release characteristics of SG-NCTD-LIP. Furthermore, in vitro cytotoxicity of SG-NCTD-LIP in HepG2 cells was determined by MTT assay.

Materials and methods

Materials

NCTD (>99%) was purchased from Sunray Pharmaceutical Co., Ltd. (Suzhou, China). SG (>98%) was obtained from Xi'an Realin Biotechnology Co., Ltd. (Xi'an, China). Egg phosphatidylcholine (EPC) was supplied by Lipoid GmbH (Ludwigshafen, Germany). Cholesterol was purchased from Beijing Solarbio Science & Technology Co., Ltd. (Beijing, China). HPLC-grade acetonitrile was purchased from Sigma-Aldrich (Merck KGaA, Darmstadt, Germany). All other chemicals used in this study were of analytical grade.

Preparation of SG-NCTD-LIP

Liposomes were prepared by ethanol injection method (Fig. 3) (37,38). EPC (0.1–0.4%), cholesterol (0.03–0.20%) and SG (0.04%) were dissolved in 2 ml absolute ethanol. The aqueous phase was prepared by dissolving NCTD (0.02–0.08%) in 15 ml PBS (pH 7.0) and heating in a water bath at 37°C. The 2 ml ethanol phase was immediately injected into the heated aqueous solution through a fine needle under magnetic stirring. The liposome dispersions were incubated at between 50°C for 30 min with gentle stirring.

Determination of NCTD content and EE

SG-NCTD-LIP was separated from the free drug by equilibrium dialysis (the dialysis membrane bag molecular weight cut-off of 8,000–14,000). The liposome fraction was obtained and the liposomes were ruptured in methanol for drug solubilisation. The resulting solution was sonicated (150 W at 25°C) for 5 min and filtered through polytetrafluorethylene membranes (0.22 µm). The drug concentration was determined using a reversed-phase high-performance liquid chromatography (RP-HPLC) system (LC-20AT; Shimadzu Corporation, Kyoto, Japan) at 25°C and 98% NCTD was used as a quantification standard. The mobile phase was acetonitrile/water (10:90, v/v; pH 3.1) and an isocratic elution was performed using a WondaCract ODS-2 column (5 µm, 4.6×250 mm; Shimadzu Corporation) with a flow rate of 0.8 ml/min. NCTD was detected at 220 nm. The EE was determined by dividing the amount of drug in the liposome fraction by the amount of drug in the total fractions.

Optimization of SG-NCTD-LIP formulation

Using the ethanol injection method, several factors were trialed to achieve optimal formulation, including NCTD-phospholipid mass ratio (factor A), phospholipid concentration (factor B), incubation temperature (factor C) and cholesterol-phospholipid mass ratio (factor D) during the fabrication process. Only one factor was replaced in each series of experiments. When changing the amount of phospholipids, the NCTD-phospholipid mass ratio was 1:5, cholesterol-phospholipid mass ratio was 1:7 and incubation temperature was 50°C. When changing the amount of NCTD, the phospholipid concentration was 0.24%, cholesterol-phospholipid mass ratio was 1:7 and incubation temperature was 50°C. When changing cholesterol-phospholipid mass ratio, the phospholipid concentration was 0.24%, NCTD-phospholipid mass ratio was 1:20 and incubation temperature was 50°C. When changing incubation temperature, the phospholipid concentration was 0.24%, NCTD-phospholipid mass ratio was 1:20 and cholesterol-phospholipid mass ratio was 1:5. Based on the investigation of factors, the four aforementioned factors were selected, and three levels of each factor were designated for the orthogonal design, with the EE as the investigating indicator to screen the formulation.

SG-NCTD-LIP size and polydispersity index (PDI)

The particle size and PDI of SG-NCTD-LIP were determined at 25°C by dynamic light scattering (Nano-ZS; Malvern Instruments, Ltd., Malvern, UK). Prior to measurement, the liposome dispersions were diluted 10 times with distilled water. Measurements were performed in triplicate on independent formulations at a detection angle of 90°.

Transmission electron microscopy (TEM) measurement of SG-NCTD-LIP

A diluted SG-NCTD-LIP sample (0.018 mg/ml; 10 µl) was placed on a copper grid and air-dried at room temperature. Subsequently, a drop of 1% (w/v) aqueous solution of phosphotungstic acid was added for negative staining; the sample was dried at room temperature for 20 min. The analyses of the vesicle shape were carried out on a JEM-2100F transmission electron microscope (JEOL, Ltd., Tokyo, Japan) with an acceleration of 150 kV.

SG-NCTD-LIP stability studies

SG-NCTD-LIP suspension stability was assessed over 3 months of storage at 4°C by means of particle size, PDI and EE measurements.

In vitro drug release study

In vitro drug release was performed as described previously (39). Dialysis tubing (molecular weight cut off, 8,000–14,000 g/mol) was incubated in distilled water for 12 h at room temperature. SG-NCTD-LIP (0.18 mg/ml. 8 ml) and NCTD solution (0.18 mg/ml, 8 ml) were placed in separate dialysis tubing and each incubated with stirring in 80 ml PBS (pH 7.4, release medium) at 37°C. Following 0.25, 0.5, 1, 1.5, 2, 4, 8 and 12 h incubation, 0.5 ml release medium was collected and replaced with an equal volume of fresh medium. NCTD concentration was analyzed by RP-HPLC.

Cell culture

HepG2, an immortalized cell line consisting of hepatoblastoma cells, was obtained from the Laboratory of Hepatobiliary and Pancreatic Surgery (Affiliated Hospital of Guilin Medical University, Guilin, China). Cells were cultured in Dulbecco's modified Eagle's medium supplemented with 10% fetal bovine serum (FBS; both Gibco; Thermo Fisher Scientific, Inc., Waltham, MA, USA), 100 U/ml penicillin and 100 µg/ml streptomycin under 5% CO2 atmosphere at 37°C for 24 h.

Cytotoxicity study

3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assays were used to study the proliferative effect of SG-NCTD-LIP. HepG2 cells were plated in 96-well culture plates at 4,000 cells/well. After overnight incubation at 37°C, cells were treated with varying concentrations (2.5, 5, 10, 20 and 40 µg/ml) of SG-modified blank liposomes (SG-LIP), NCTD solution, liposomes loaded with NCTD (NCTD-LIP) or SG-NCTD-LIP and were incubated for 48 h at 37°C. MTT (Sigma-Aldrich; Merck KGaA) solution (20 µl; 5 mg/ml) was added to each well. Following 4 h incubation at 37°C, the medium was replaced with 150 µl dimethyl sulfoxide to dissolve formazan crystals. Optical densities (ODs) were measured at 490 nm with an ELISA reader. The half-maximal inhibitory concentration (IC50) was calculated using SPSS (version 22.0; IBM Corp., Armonk, NY, USA). The inhibition ratio (IR) was calculated as follows: IR=1-OD of cells treated with sample/OD of culture medium.

Statistical analysis

Data were analyzed using SPSS software (version 22.0; IBM Corp., Armonk, NY, USA) and results are expressed as mean ± standard deviation. One-way analysis of variance followed by a Fisher's Least Significant Difference post-hoc test was used to assess the significance of the differences among various groups. Correlation coefficient (R) is a variable that demonstrated the degree of linear correlation between variables. P<0.05 was considered to indicate a statistically significant difference.

Results and Discussion

Effect of different preparation variables on the EE of SG-NCTD-LIP

The results presented in Fig. 4A identified the highest EE at 0.24% phospholipid concentration. However, excessively high phospholipid resulted in the aggregation of phospholipids and decreased EE. It was revealed that the EE of SG-NCTD-LIP was lowered from 25 to 16% when increasing the amount of NCTD; the highest EE was observed for a 1:20 NCTD-phospholipid ratio (Fig. 4B). Cholesterol is a lipid used to improve liposome stability in vitro and in vivo (40). The results indicated that the highest EE was achieved at a 1:5 cholesterol-phospholipid mass ratio (Fig. 4C). At higher ratios, cholesterol may induce rapid vesicle aggregation. Its rigidity restricts the lipid bilayer flexibility and prevents NCTD accumulation inside the vesicle. The optimum incubation temperature was determined to be 40°C (Fig. 4D).

Optimization of the SG-NCTD-LIP formulation by orthogonal design

Four factors, NCTD-phospholipid mass ratio (factor A), phospholipid concentration (factor B), incubation temperature (factor C) and cholesterol-phospholipid mass ratio (factor D) were selected as main influencing factors. Each factor was studied at three levels and the EE was selected as the investigated indicator of the formulation. The orthogonal factors and levels are presented in Table I. Nine formulations were tested, according to the L9 (34) orthogonal table. Table II displays the orthogonal test results and the variance analysis is presented in Table III. According to the extremum values (R) in Table II, the importance of the factors may be ranked as A>B>C>D. The optimum formulation composition was identified as A1B1C2D3, with 1:5 NCTD-phospholipid mass ratio, 0.4% phospholipid and 1:7 cholesterol-phospholipid mass ratio at an incubation temperature of 50°C. The analysis of variance indicated that factors A, B and C had a significant impact on the experimental outcome (Table III). According to the F-value, the impact of the factors may be scored as A>B>C>D, which supports earlier findings. Preparation and analysis of the optimal formulation mentioned above were repeated three times and the EE was 27.80±2.18%. Prior to optimization of the NCTD-LIP formulation, literature was consulted (4144) and combined with preliminary experiments, which investigated the effect of different masses of SG on the size, morphology, EE and stability of liposomes. Following this, 0.04% SG was identified as an appropriate amount of drug to be added, without affecting the formulation parameters of the liposome.

Table I.

Factors and levels for optimization using orthogonal design.

Table I.

Factors and levels for optimization using orthogonal design.

Factor

LevelAB (%)C (°C)D
11:50.040401:3
21:150.025501:5
31:300.015601:7

[i] A, NCTD-phospholipid mass ratio; B, phospholipid concentration; C, incubation temperature; D, cholesterol-phospholipid mass ratio.

Table II.

Results of orthogonal testing.

Table II.

Results of orthogonal testing.

No.AB (%)C (°C)DEE (%)
11.001.001.001.0025.88
21.002.002.002.0023.40
31.003.003.003.0022.65
42.001.002.003.0027.63
52.002.003.001.0020.26
62.003.001.002.0023.96
73.001.003.002.0017.20
83.002.001.003.0015.53
93.003.002.001.0017.72
K171.9370.7165.3763.86N/A
K271.8559.1968.7564.56N/A
K350.4564.3360.1165.81N/A
R21.4811.528.641.95N/A

[i] A, NCTD-phospholipid mass ratio; B, phospholipid concentration; C, incubation temperature; D, cholesterol-phospholipid mass ratio; EE, encapsulation efficiency; Kn, sum of EE values at level n; R=Kmax-Kmin.

Table III.

Variance analysis.

Table III.

Variance analysis.

SourceSfMS F-statisticP-value
A102.15251.08157.02<0.01
B22.20211.1034.13<0.05
C12.6426.3219.43<0.05
D0.6520.331.00>0.05
Error0.6520.33N/AN/A

[i] S, sum of deviation square; f, degree of freedom; MS, mean square; A, NCTD-phospholipid mass ratio; B, phospholipid concentration; C, incubation temperature; D, cholesterol-phospholipid mass ratio.

SG-NCTD-LIP size and morphology

Nanoparticles, between 70–200 nm, function as drug delivery systems and are usually in the circulation for longer than free drugs (45,46). In addition, such nanoparticles escape RES clearance, while larger particles (>200 nm) may be removed by the system. A small particle size (<200 nm), below the pore size of leaky vasculatures, along with poor lymphatic drainage may provide a suitable condition for the accumulation and localization in the tumor (47). The average particle size at the optimal formulation after 3 months of storage at 4°C was 87.5 nm with a PDI of 0.151 (Fig. 5). The morphological character of SG-NCTD-LIPs was evaluated by TEM. Liposomes were spherical, no aggregation or fusion occurred and the size was ~100 nm.

Stability of liposomes SG-NCTD-LIPs

Particle size and EE were evaluated following 3 months of storage at 4°C to study stability. Initially, the average particle size was 87.5 nm and EE was 27.80%. There were no significant changes in the average particle size and EE following 3 months at 4°C, the average particle size was 82.9 nm and EE was 27.28%. The SG-NCTD-LIP stability was attributed to the presence of cholesterol, which increased the rigidity of the liposomal membrane and reduced permeability (48). The results demonstrated that SG-NCTD-LIPs were stable at 4°C for 3 months. However, the biological efficacy of SG-NCTD-LIPs following storage was not evaluated.

In vitro release of NCTD from SG-NCTD-LIP

The in vitro release rate evaluation describes an essential step for drug development and quality control, which may reflect on the in vivo drug performance. Q is used to denote the cumulative release percentage. The release rate of NCTD from SG-NCTD-LIP was significantly decreased compared with the free NCTD solution 1.5–12 h after incubation (Fig. 6). SG-NCTD-LIP may prolong the release of NCTD from liposomes. The release process of NCTD from SG-NCTD-LIP may be divided into two phases: In the first 4 h, the release rate was high, with 65% of the total dose released. Unencapsulated NCTD and NCTD coordinating to the surface of the lipid bilayer may be responsible for the rapid release. This phase was defined as a rapid release phase. It was followed by a steady release, known as the slow release phase. To investigate the release properties of SG-NCTD-LIP, release data were fitted using various release models, including zero-order, first-order, Higuchi (49) and Weibull (50) kinetic models, and the optimal fit was determined by correlation coefficient. The fitting equations of the release curves are presented in Table IV. The results indicated that the release from SG-NCTD-LIP follows a first order model.

Table IV.

Release curve-fitting equations of norcantharidin loaded liposomes modified with stearyl glycyrrhetinate.

Table IV.

Release curve-fitting equations of norcantharidin loaded liposomes modified with stearyl glycyrrhetinate.

ModelFitting equationR
Zero-order Q=0.0972t+0.23030.9190
First order ln(1-Q)=−0.3336t−0.21100.9902
Higuchi Q=0.3732t1/2+0.03500.9764
Weibull ln(1/1-Q)=0.6040 lnt+0.74810.9561

[i] R, correlation coefficient; Q, accumulative release rate; t, time.

In vitro cytotoxicity study

The cytotoxicity of SG-LIP, free NCTD, NCTD-LIP and SG-NCTD-LIP towards HepG2 cells was evaluated using the MTT cytotoxicity assay. SG-LIP was assessed as a control and the concentration of SG in SG-LIP was equal to its concentration in SG-NCTD-LIP. Following incubation for 48 h, an IR value of ~6.35% was determined for SG-LIP, indicating no significant cytotoxicity against HepG2 cells (data not shown). The inhibitory effect of the samples increased with the NCTD concentration; all treatments revealed dose-dependent cytotoxic activity (Fig. 7). NCTD-LIP and SG-NCTD-LIP demonstrated significantly increased toxicity compared with free NCTD. In addition, when NCTD concentration was 5, 10 and 20 µg/ml, SG-NCTD-LIP significantly increased cytotoxicity compared with NCTD-LIP after 48 h incubation. The IC50 of SG-NCTD-LIP, NCTD-LIP and free NCTD were 16.93, 24.03 and 49.79 µg/ml, respectively. SG-NCTD-LIP was 1.42-fold more cytotoxic compared with NCTD-LIP. SG modification increased the cytotoxicity of the liposomes, which may be associated with the interaction between SG exposed on the liposome surface and GA receptors on the cell membrane.

Conclusions

SG-NCTD-LIP was successfully prepared using the ethanol injection method and the determined properties, including particle size, EE, release profile and stability, may meet the potential requirements of liver cancer therapy based on the in vitro experiments, which overcome the limitations of conventional chemotherapy by improving the bioavailability and stability of the drug molecules, and minimizing side effects by site-specific and targeted delivery of the drugs (5153). The in vitro cytotoxicity study confirmed that SG modification of NCTD-LIP enhanced the inhibitory effects on hepatoblastoma cells. Therefore, SG-NCTD-LIP may be a potential drug delivery system for NCTD-targeted liver cancer therapy. Future experiments may include cytotoxicity studies against HepG2 cells compared with L02 normal liver cells, to confirm the safety and efficacy of SG-NCTD-LIP, further uptake studies and in vivo experiments.

Acknowledgements

Not applicable.

Funding

The present study was funded by grants from the National Natural Science Foundation of China (grant no. 81560653) and the Natural Science Foundation of Guangxi Province of China (grant no. 2016GXNSFAA380081).

Availability of data and materials

The datasets generated and analyzed during the current study are not publicly available due to the patent application but are available from the corresponding author on reasonable request.

Authors' contributions

WW was involved in the design of the study. JZ and WZ performed all experiments. DW performed the in vitro cytotoxicity studies. SL performed statistical analyses. All authors participated in manuscript preparation and involved in the discussion of the results. All authors have read and approved the final manuscript.

Ethics approval and consent to participate

Not applicable.

Patient consent for publication

Not applicable.

Competing interests

The authors declare that they have no competing interests.

References

1 

Bei YY, Chen XY, Liu Y, Xu JY, Wang WJ, Gu ZL, Xing KL, Zhu AJ, Chen WL, Shi LS, et al: Novel norcantharidin-loaded liver targeting chitosan nanoparticles to enhance intestinal absorption. Int J Nanomedicine. 7:1819–1827. 2012.PubMed/NCBI

2 

Chen YC, Chang SC, Wu MH, Chuang KA, Wu JY, Tsai WJ and Kuo YC: Norcantharidin reducedcyclins and cytokines production in human peripheral blood mononuclearcells. Life Sci. 84:218–226. 2009. View Article : Google Scholar : PubMed/NCBI

3 

Wei CM, Wang BJ, Ma Y, Sun ZP, Li XL and Guo RC: Pharmacokinetics and biodistributionof 3H-norcantharidin in mice. Yao Xue Xue Bao. 42:516–519. 2007.(In Chinese). PubMed/NCBI

4 

Chen YN, Chen JC, Yin SC, Wang GS, Tsauer W, Hsu SF and Hsu SL: Effect ormechanisms of norcantharidin-induced mitotic arrest and apoptosis inhuman hepatoma cells. Int J Cancer. 100:158–165. 2002. View Article : Google Scholar : PubMed/NCBI

5 

McCluskey A and Sakoff JA: Small molecule inhibitors of serine/threonine protein phosphatases. Mini Rev Med Chem. 1:43–55. 2001. View Article : Google Scholar : PubMed/NCBI

6 

Zheng LC, Yang MD, Kuo CL, Lin CH, Fan MJ, Chou YC, Lu HF, Huang WW, Peng SF and Chung JG: Norcantharidin-induced apoptosis of ags human gastric cancer cells through reactive oxygen species production, and caspase- and mitochondria-dependent signaling pathways. Anticancer Res. 36:6031–6042. 2016. View Article : Google Scholar : PubMed/NCBI

7 

Zhang S, Li G, Ma X, Wang Y, Liu G, Feng L, Zhao Y, Zhang G, Wu Y, Ye X, et al: Norcantharidin enhances ABT-737-induced apoptosis in hepatocellular carcinoma cells by transcriptional repression of Mcl-1. Cell Signal. 24:1803–1809. 2012. View Article : Google Scholar : PubMed/NCBI

8 

Wan XY, Zhai XF, Jiang YP, Han T, Zhang QY and Xin HL: Antimetastatic effects of norcantharidin on hepatocellular carcinoma cells by up-regulating FAM46C expression. Am J Transl Res. 9:155–166. 2017.PubMed/NCBI

9 

Xiong X, Wu M, Zhang H, Li J, Lu B, Guo Y, Zhou T, Guo H, Peng R, Li X, et al: Atg5 siRNA inhibits autophagy and enhances norcantharidin-induced apoptosis in hepatocellular carcinoma. Int J Oncol. 47:1321–1328. 2015. View Article : Google Scholar : PubMed/NCBI

10 

Lin X, Zhang B, Zhang K, Zhang Y, Wang J, Qi N, Yang S, He H and Tang X: Preclinical evaluations of norcantharidin-loaded intravenous lipid microspheres with low toxicity. Expert Opin Drug Deliv. 9:1449–1462. 2012. View Article : Google Scholar : PubMed/NCBI

11 

Xie J, Zhang Y, Hu X, Lv R, Xiao D, Jiang L and Bao Q: Norcantharidin inhibits Wnt signal pathway via promoter demethylation of WIF-1 in human non-small cell lung cancer. Med Oncol. 32:1452015. View Article : Google Scholar : PubMed/NCBI

12 

Liu MC, Liu L, Wang XR, Shuai WP, Hu Y, Han M and Gao JQ: Folate receptor-targeted liposomes loaded with a diacid metabolite of norcantharidin enhance antitumor potency for H22 hepatocellular carcinoma both in vitro and in vivo. Int J Nanomedicine. 11:1395–1412. 2016. View Article : Google Scholar : PubMed/NCBI

13 

Zeng Q and Sun M: Poly(lactide-co-glycolide) nanoparticles as carriers for norcantharidin. Materials Science and Engineering: C. 29:708–713. 2009. View Article : Google Scholar

14 

Ding XY, Hong CJ, Liu Y, Gu ZL, Xing KL, Zhu AJ, Chen WL, Shi LS, Zhang XN and Zhang Q: Pharmacokinetics, tissue distribution, and metabolites of a polyvinylpyrrolidone-coated norcantharidin chitosan nanoparticle formulation in rats and mice, using LC-MS/MS. Int J Nanomedicine. 7:1723–1735. 2012.PubMed/NCBI

15 

Yan D, Ni LK, Chen HL, Chen LC, Chen YH and Cheng CC: Amphiphilic nanoparticles of resveratrol-norcantharidin to enhance the toxicity in zebrafish embryo. Bioorg Med Chem Lett. 26:774–777. 2016. View Article : Google Scholar : PubMed/NCBI

16 

Ding XY, Hong CJ and Zhou X: Mechanism of Polyvinylpyrrolidone-coated norcantharidin chitosan nanoparticle. Current Nanoscience. 9:401–406. 2013. View Article : Google Scholar

17 

Torchilin VP: Recent advances with liposomes as pharmaceutical carriers. Nat Rev Drug Discov. 4:145–160. 2005. View Article : Google Scholar : PubMed/NCBI

18 

Bangham AD, Standish MM and Watkins JC: Diffusion of univalent ions across the lamellae of swollen phospholipids. J Mol Biol. 13:238–252. 1965. View Article : Google Scholar : PubMed/NCBI

19 

Irie T, Watarai S and Kodama H: Humoral immune response of carp (Cyprinus carpio) induced by oral immunization with liposome-entrapped antigen. Dev Comp Immunol. 27:413–421. 2003. View Article : Google Scholar : PubMed/NCBI

20 

Kirby C, Clarke J and Gregoriadis G: Effect of the cholesterol content of small unilamellar liposomes on their Stability in vivo and in vitro. Biochem J. 186:591–598. 1980. View Article : Google Scholar : PubMed/NCBI

21 

Zhang L, Gu FX, Chan JM, Wang AZ, Langer RS and Farokhzad OC: Nanoparticles in medicine: Therapeutic applications and developments. Clin Pharmacol Ther. 83:761–769. 2008. View Article : Google Scholar : PubMed/NCBI

22 

Ara MN, Matsuda T, Hyodo M, Sakurai Y, Hatakeyama H, Ohga N, Hida K and Harashima H: An aptamer ligand based liposomal nanocarrier system that targets tumor endothelial cells. Biomaterials. 35:7110–7120. 2014. View Article : Google Scholar : PubMed/NCBI

23 

Kang H, O'Donoghue MB, Liu H and Tan W: A liposome-based nanostructure for aptamer directed delivery. Chem Commun (Camb). 46:249–251. 2010. View Article : Google Scholar : PubMed/NCBI

24 

Moghimi SM, Hunter AC and Murray JC: Long-circulating andtarget-specific nanoparticles: Theory to practice. Pharmacol Rev. 53:283–318. 2001.PubMed/NCBI

25 

Maeda H: Tumor-selective delivery of macromolecular drugs via the EPReffect: Background and future prospects. Bioconjug Chem. 21:797–802. 2010. View Article : Google Scholar : PubMed/NCBI

26 

Kibria G, Hatakeyama H, Ohga N, Hida K and Harashima H: Dual-ligand modification of PEGylated liposomes shows better cell selectivity and efficient gene delivery. J Control Release. 153:141–148. 2011. View Article : Google Scholar : PubMed/NCBI

27 

Darvishi B, Manoochehri S, Esfandyari-Manesh M, Samadi N, Amini M, Atyabi F and Dinarvand R: Enhanced cellular cytotoxicity and antibacterial activity of 18-β-Glycyrrhetinic Acidby Albumin-conjugated PLGA Nanoparticles. Drug Res. 65:617–623. 2015. View Article : Google Scholar

28 

Kuang P, Zhao W, Su W, Zhang Z, Zhang L, Liu J, Ren G and Wang X: 18β-glycyrrhetinic acid inhibits hepatocellular carcinoma development by reversing hepatic stellate cell-mediated immunosuppression in mice. IntJ Cancer. 132:1831–1841. 2013. View Article : Google Scholar

29 

Manns MP, Wedemeyer H, Singer A, Khomutjanskaja N, Dienes HP, Roskams T, Goldin R, Hehnke U and Inoue H: European SNMC Study Group: Glycyrrhizin in patients who failed previous interferon alpha-basedtherapies: Biochemical and histological effects after 52 weeks. J Viral Hepat. 19:537–546. 2012. View Article : Google Scholar : PubMed/NCBI

30 

Tang ZH, Li T, Chang LL, Zhu H, Tong YG, Chen XP, Wang YT and Lu JJ: Glycyrrhetinic Acid triggers a protectiveautophagy by activation of extracellular regulated protein kinases in hepatocellular carcinoma cells. J Agric Food Chem. 62:11910–11916. 2014. View Article : Google Scholar : PubMed/NCBI

31 

Irie A, Fukui T, Negishi M, Nagata N and Ichikawa A: Glycyrrhetinic acid bound to 11 beta-hydroxysteroid dehydrogenase in rat liver microsomes. Biochim Biophys Acta. 1160:229–234. 1992. View Article : Google Scholar : PubMed/NCBI

32 

Negishi M, Irie A, Nagata N and Ichikawa A: Specific binding of glycyrrhetinic acid to the rat liver membrane. Biochim Biophys Acta. 1066:77–82. 1991. View Article : Google Scholar : PubMed/NCBI

33 

Tian Q, Zhang CN, Wang XH, Wang W, Huang W, Cha RT, Wang CH, Yuan Z, Liu M, Wan HY and Tang H: Glycyrrhetinic acid-modified chitosan/poly (ethylene glycol) nanoparticles for liver-targeted delivery. Biomaterials. 31:4748–4756. 2010. View Article : Google Scholar : PubMed/NCBI

34 

Tian Q, Wang XH, Wang W, Zhang CN, Wang P and Yuan Z: Self-assembly and liver targeting of sulfated chitosan nanoparticles functionalized with glycyrrhetinic acid. Nanomedicine. 8:870–879. 2012. View Article : Google Scholar : PubMed/NCBI

35 

Zhang J, Zhang M, Ji J, Fang X, Pan X, Wang Y, Wu C and Chen M: Glycyrrhetinic acid-mediated polymeric drug delivery targeting the acidic microenvironment of hepatocellular carcinoma. Pharm Res. 32:3376–3390. 2015. View Article : Google Scholar : PubMed/NCBI

36 

Chintharlapalli S, Papineni S, Jutooru I, McAlees A and Safe S: Structure-dependent activity of glycyrrhetinic acid derivatives asperoxisome proliferator-activated receptor {gamma} agonists in colon cancer cells. Mol Cancer Ther. 6:1588–1598. 2007. View Article : Google Scholar : PubMed/NCBI

37 

Marzban E, Alavizadeh SH, Ghiadi M, Khoshangosht M, Khashayarmanesh Z, Abbasi A and Jaafari MR: Optimizing the therapeutic efficacy of cisplatin PEGylated liposomes via incorporation of different DPPG ratios: In vitro and in vivo studies. Colloids Surf B Biointerfaces. 136:885–891. 2015. View Article : Google Scholar : PubMed/NCBI

38 

Chi L, Na MH, Jung HK, Vadevoo SM, Kim CW, Padmanaban G, Park TI, Park JY, Hwang I, Park KU, et al: Enhanced delivery of liposomes to lung tumor through targeting interleukin-4 receptor on both tumor cells and tumor endothelial cells. J Control Release. 209:327–336. 2015. View Article : Google Scholar : PubMed/NCBI

39 

Liu D, Xing J, Xiong F, Yang F and Gu N: Preparation and in vivo safety evaluations of antileukemichomoharringtonine-loaded PEGylated liposomes. Drug Dev Ind Pharm. 43:652–660. 2017. View Article : Google Scholar : PubMed/NCBI

40 

Kirby C, Clarke J and Gregoriadis G: Effect of the cholesterol content of small unilamellar liposomeson their stability in vivo and in vitro. Biochem J. 186:591–598. 1980. View Article : Google Scholar : PubMed/NCBI

41 

Li J, Xu H, Ke X and Tian J: The anti-tumor performance of docetaxel liposomes surface-modified with glycyrrhetinic acid. J Drug Targeting. 20:467–473. 2012. View Article : Google Scholar

42 

Chen J, Jiang H, Wu Y, Li Y and Gao Y: A novel glycyrrhetinic acid-modified oxaliplatin liposome for liver-targeting and in vitro/vivo evaluation. Drug Des Dev Ther. 9:2265–2275. 2015.

43 

Cheng M, Gao X, Wang Y, Chen H, He B, Xu H, Li Y, Han J and Zhang Z: Synthesis of glycyrrhetinic acid-modified chitosan5-fluorouracil nanoparticles and its inhibition of liver cancercharacteristics in vitro and in vivo. Mar Drugs. 11:3517–3536. 2013. View Article : Google Scholar : PubMed/NCBI

44 

Tian Q, Wang XH, Wang W, Zhang CN, Wang P and Yuan Z: Self-assembly and liver targeting of sulfated chitosannanoparticles functionalized with glycyrrhetinic acid. Nanomedicine. 8:870–879. 2012. View Article : Google Scholar : PubMed/NCBI

45 

Ossipov DA: Nanostructured hyaluronic acid-based materials for active delivery tocancer. Expert Opin Drug Deliv. 7:681–703. 2010. View Article : Google Scholar : PubMed/NCBI

46 

Yang T, Choi MK, Cui FD, Kim JS, Chung SJ, Shim CK and Kim DD: Preparationand evaluation of paclitaxel-loaded PEGylated immunoliposome. J Control Release. 120:169–177. 2007. View Article : Google Scholar : PubMed/NCBI

47 

Ravar F, Saadat E, Gholami M, Dehghankelishadi P, Mahdavi M, Azami S and Dorkoosh FA: Hyaluronic acid-coated liposomes for targeted delivery of paclitaxel, in-vitro characterization and in-vivo evaluation. J Control Release. 229:10–22. 2016. View Article : Google Scholar : PubMed/NCBI

48 

Fan MH, Xu SY, Xia SQ and Zhang XM: Preparation of salidroside nano-liposomes by ethanol injection method and in vitro release study. Eur Food Res Technol. 227:167–174. 2008. View Article : Google Scholar

49 

Higuchi T: Mechanism of sustained-action medication. Theoretical analysis of rate of release of solid drugs dispersed in solid matrices. J Pharm Sci. 52:1145–1149. 1963. View Article : Google Scholar : PubMed/NCBI

50 

Weibull W: A statistical distribution of wide applicability. J Appl Mech. 18:293–297. 1951.

51 

Dutta R and Mahato RI: Recent advances in hepatocellular carcinoma therapy. Pharmacol Ther. 173:106–117. 2017. View Article : Google Scholar : PubMed/NCBI

52 

Sarfraz M, Afzal A, Raza SM, Bashir S, Madni A, Khan MW, Ma X and Xiang G: Liposomal co-delivered oleanolic acid attenuates doxorubicin-induced multi-organ toxicity in hepatocellular carcinoma. Oncotarget. 8:47136–47153. 2017. View Article : Google Scholar : PubMed/NCBI

53 

Himanshu P, Radha R and Vishnu A: Liposome and their applications in cancer therapy. Braz Arch Boil Technol. 59:e161504772016.

Related Articles

Journal Cover

September-2018
Volume 16 Issue 3

Print ISSN: 1792-0981
Online ISSN:1792-1015

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Zhu J, Zhang W, Wang D, Li S and Wu W: Preparation and characterization of norcantharidin liposomes modified with stearyl glycyrrhetinate. Exp Ther Med 16: 1639-1646, 2018
APA
Zhu, J., Zhang, W., Wang, D., Li, S., & Wu, W. (2018). Preparation and characterization of norcantharidin liposomes modified with stearyl glycyrrhetinate. Experimental and Therapeutic Medicine, 16, 1639-1646. https://doi.org/10.3892/etm.2018.6416
MLA
Zhu, J., Zhang, W., Wang, D., Li, S., Wu, W."Preparation and characterization of norcantharidin liposomes modified with stearyl glycyrrhetinate". Experimental and Therapeutic Medicine 16.3 (2018): 1639-1646.
Chicago
Zhu, J., Zhang, W., Wang, D., Li, S., Wu, W."Preparation and characterization of norcantharidin liposomes modified with stearyl glycyrrhetinate". Experimental and Therapeutic Medicine 16, no. 3 (2018): 1639-1646. https://doi.org/10.3892/etm.2018.6416