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Preparation and characterization of norcantharidin liposomes modified with stearyl glycyrrhetinate

  • Authors:
    • Jing Zhu
    • Wei Zhang
    • Dandan Wang
    • Suzhen Li
    • Wei Wu
  • View Affiliations / Copyright

    Affiliations: Department of Pharmaceutics, College of Pharmacy, Guilin Medical University, Guilin, Guangxi 541004, P.R. China, Clinical Experimental Teaching Center, Affiliated Hospital of Guilin Medical University, Guilin, Guangxi 541004, P.R. China
    Copyright: © Zhu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 1639-1646
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    Published online on: July 6, 2018
       https://doi.org/10.3892/etm.2018.6416
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Abstract

In the current study, norcantharidin (NCTD)-loaded liposomes (LIPs) modified with stearyl glycyrrhetinate (SG; SG‑NCTD‑LIP) were prepared by the ethanol injection method. To increase the drug encapsulation efficiency (EE), the formulation of NCTD‑LIP was optimized by single factor test and orthogonal design. The release of NCTD in vitro from SG‑NCTD‑LIP was evaluated by equilibrium dialysis. The cytotoxicity of SG‑NCTD‑LIP in HepG2 was investigated by MTT assay. The results revealed that the EE of liposomes was ~27.80%, the average SG‑NCTD‑LIP was 87.5 nm, the in vitro NCTD release from SG‑NCTD‑LIP was delayed compared with NCTD in solution and the drug‑release kinetic followed a first‑order model. MTT assays revealed increased cytotoxicity activity against HepG2 cells for SG‑NCTD‑LIP compared with free NCTD. In conclusion, SG‑NCTD‑LIP prepared in the present study may be a promising liposomal drug delivery system for anticancer drugs in liver‑targeting therapy.
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Copy and paste a formatted citation
Spandidos Publications style
Zhu J, Zhang W, Wang D, Li S and Wu W: Preparation and characterization of norcantharidin liposomes modified with stearyl glycyrrhetinate. Exp Ther Med 16: 1639-1646, 2018.
APA
Zhu, J., Zhang, W., Wang, D., Li, S., & Wu, W. (2018). Preparation and characterization of norcantharidin liposomes modified with stearyl glycyrrhetinate. Experimental and Therapeutic Medicine, 16, 1639-1646. https://doi.org/10.3892/etm.2018.6416
MLA
Zhu, J., Zhang, W., Wang, D., Li, S., Wu, W."Preparation and characterization of norcantharidin liposomes modified with stearyl glycyrrhetinate". Experimental and Therapeutic Medicine 16.3 (2018): 1639-1646.
Chicago
Zhu, J., Zhang, W., Wang, D., Li, S., Wu, W."Preparation and characterization of norcantharidin liposomes modified with stearyl glycyrrhetinate". Experimental and Therapeutic Medicine 16, no. 3 (2018): 1639-1646. https://doi.org/10.3892/etm.2018.6416
Copy and paste a formatted citation
x
Spandidos Publications style
Zhu J, Zhang W, Wang D, Li S and Wu W: Preparation and characterization of norcantharidin liposomes modified with stearyl glycyrrhetinate. Exp Ther Med 16: 1639-1646, 2018.
APA
Zhu, J., Zhang, W., Wang, D., Li, S., & Wu, W. (2018). Preparation and characterization of norcantharidin liposomes modified with stearyl glycyrrhetinate. Experimental and Therapeutic Medicine, 16, 1639-1646. https://doi.org/10.3892/etm.2018.6416
MLA
Zhu, J., Zhang, W., Wang, D., Li, S., Wu, W."Preparation and characterization of norcantharidin liposomes modified with stearyl glycyrrhetinate". Experimental and Therapeutic Medicine 16.3 (2018): 1639-1646.
Chicago
Zhu, J., Zhang, W., Wang, D., Li, S., Wu, W."Preparation and characterization of norcantharidin liposomes modified with stearyl glycyrrhetinate". Experimental and Therapeutic Medicine 16, no. 3 (2018): 1639-1646. https://doi.org/10.3892/etm.2018.6416
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