Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Experimental and Therapeutic Medicine
Join Editorial Board Propose a Special Issue
Print ISSN: 1792-0981 Online ISSN: 1792-1015
Journal Cover
September-2018 Volume 16 Issue 3

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
September-2018 Volume 16 Issue 3

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article Open Access

Mitochondrial damage in hippocampal neurons of rats with epileptic protein expression of Fas and caspase-3

  • Authors:
    • Junqiang Feng
    • Lifang Feng
    • Guiru Zhang
  • View Affiliations / Copyright

    Affiliations: Department of Neurology, Daqing Longnan Hospital, Daqing, Heilongjiang 163453, P.R. China, Department of Family Practice, Binzhou People's Hospital, Binzhou, Shandong 256600, P.R. China, Department of Neurology, Shandong Provincial Hospital Affiliated to Shandong University, Jinan, Shandong 250022, P.R. China
    Copyright: © Feng et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 2483-2489
    |
    Published online on: July 13, 2018
       https://doi.org/10.3892/etm.2018.6439
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

Epilepsy model in rats was established to observe the behavior and pathological changes, and to detect mitochondrial dysfunction, exploring its possible molecular mechanisms. The epileptic status of Sprague-Dawley (SD) rats was induced by intraperitoneal injection of lithium chloride, and the change of behavior was recorded. Electroencephalogram (EEG) was used to measure the abnormal discharge of neurons in rats. The brain tissue was fixed with polyformaldehyde and the paraffin sections were prepared, and the damage of the hippocampal neurons was observed with Nissl staining. Mitochondrial ATP and mitochondrial DNA were examined to assess mitochondrial dysfunction. Finally, qPCR and western blot analysis were used to detect mRNA and protein expression of fatty acid synthetase (Fas), Fas ligand (FasL) and caspase-3 in rat hippocampal neurons. The correlation between the mitochondrial dysfunction of rat hippocampal neurons and Fas and caspase-3 was analyzed. Compared with the normal group rats, the model group showed typical seizures, which were determined by the Racine attack score. EEG of the hippocampus of the model group was recorded in cluster in model group rats. Nissl staining showed a different degree of damage to the hippocampal neurons in the model group compared with normal rats. The mitochondrial ATP content and DNA content of rat hippocampal neurons in the model group were significantly lower than that of normal rats (P<0.01). The qPCR and western blot results showed that the mRNA and protein expression levels of Fas, FasL and caspase-3 were significantly increased in the hippocampus of rat model group (P<0.01). The expression level of Fas and caspase-3 in hippocampal tissues of rats was negatively correlated with mitochondrial DNA content. In conclusion, seizures cause damage of neuron mitochondria in rat hippocampus leading to death of hippocampal neurons, the mitochondrial damage of hippocampal neurons in epileptic rats was closely related to the expression of Fas and caspase-3.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

Figure 6

View References

1 

Sahoo SS, Lhatoo SD, Gupta DK, Cui L, Zhao M, Jayapandian C, Bozorgi A and Zhang GQ: Epilepsy and seizure ontology: Towards an epilepsy informatics infrastructure for clinical research and patient care. J Am Med Inform Assoc. 24:82–89. 2014. View Article : Google Scholar

2 

Liu J, Reeves C, Michalak Z, Coppola A, Diehl B, Sisodiya SM and Thom M: Evidence for mTOR pathway activation in a spectrum of epilepsy-associated pathologies. Acta Neuropathol Commun. 2:712014. View Article : Google Scholar : PubMed/NCBI

3 

Speed D, OBrien TJ, Palotie A, Shkura K, Marson AG, Balding DJ and Johnson MR: Describing the genetic architecture of epilepsy through heritability analysis. Brain. 137:55–69. 2014. View Article : Google Scholar

4 

Kaiboriboon K, Bakaki PM, Lhatoo SD and Koroukian S: Incidence and prevalence of treated epilepsy among poor health and low-income Americans. Neurology. 80:1942–1949. 2013. View Article : Google Scholar : PubMed/NCBI

5 

Ying Z, Najm I, Nemes A, Pinheiro-Martins AP, Alexopoulos A, Gonzalez-Martinez J and Bingaman W: Growth-associated protein 43 and progressive epilepsy in cortical dysplasia. Ann Clin Transl Neurol. 1:453–461. 2014. View Article : Google Scholar : PubMed/NCBI

6 

Wirrell EC, Grossardt BR, Wong-Kisiel LC and Nickels KC: Incidence and classification of new-onset epilepsy and epilepsy syndromes in children in Olmsted County, Minnesota from 1980 to 2004: A population-based study. Epilepsy Res. 95:110–118. 2011. View Article : Google Scholar : PubMed/NCBI

7 

Bowen JM, Snead OC, Chandra K, Blackhouse G and Goeree R: Epilepsy care in Ontario: An economic analysis of increasing access to epilepsy surgery. Ont Health Technol Assess Ser. 12:1–41. 2012.PubMed/NCBI

8 

Bardai RJ, Lamberts RJ, Blom MT, Spanjaart AM, Berdowski J, van der Staal SR, Brouwer HJ, Koster RW, Sander JW, Thijs RD, et al: Lamberts, marieke T. epilepsy is a risk factor for sudden cardiac arrest in the general population. PLoS One. 7:14–23. 2012. View Article : Google Scholar

9 

Kovac S, Kostova Dinkova AT, Herrmann AM, Melzer N, Meuth SG and Gorji A: Metabolic and homeostatic changes in seizures and acquired epilepsy - mitochondria, calcium dynamics and reactive oxygen species. Int J Mol Sci. 18:E19352017. View Article : Google Scholar : PubMed/NCBI

10 

Fahrner JA, Liu R, Perry MS, Klein J and Chan DC: A novel de novo dominant negative mutation in DNM1L impairs mitochondrial fission and presents as childhood epileptic encephalopathy. Am J Med Genet A. 170:2002–2011. 2016. View Article : Google Scholar : PubMed/NCBI

11 

McFarland KN, Liu J, Landrian I, Zeng D, Raskin S, Moscovich M, Gatto EM, Ochoa A, Teive HA, Rasmussen A, et al: Repeat interruptions in spinocerebellar ataxia type 10 expansions are strongly associated with epileptic seizures. Neurogenetics. 15:59–64. 2014. View Article : Google Scholar : PubMed/NCBI

12 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2−ΔΔCT method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI

13 

Chang HJ, Liao CC, Hu CJ, Shen WW and Chen TL: Psychiatric disorders after epilepsy diagnosis: A population-based retrospective cohort study. PLoS One. 8:e599992013. View Article : Google Scholar : PubMed/NCBI

14 

McDonald TS, Carrasco-Pozo C, Hodson MP and Borges K: Alterations in cytosolic and mitochondrial (U-13C)glucose metabolism in a chronic epilepsy mouse model. eNeuro. Mar 9–2017.(Epub ahead of print). doi: 10.1523/ENEURO.0341-16.2017. View Article : Google Scholar : PubMed/NCBI

15 

Rowley S, Liang LP, Fulton R, Shimizu T, Day B and Patel M: Mitochondrial respiration deficits driven by reactive oxygen species in experimental temporal lobe epilepsy. Neurobiol Dis. 75:151–158. 2015. View Article : Google Scholar : PubMed/NCBI

16 

Meng DW, Liu HG, Yang AC, Zhang K and Zhang JG: Stimulation of anterior thalamic nuclei protects against seizures and neuronal apoptosis in hippocampal CA3 region of kainic acid-induced epileptic rats. Chin Med J (Engl). 129:960–966. 2016. View Article : Google Scholar : PubMed/NCBI

17 

Yamada A, Arakaki R, Saito M, Kudo Y and Ishimaru N: Dual role of Fas/FasL-mediated signal in peripheral immune tolerance. Front Immunol. 8:4032017. View Article : Google Scholar : PubMed/NCBI

18 

Liu G, Yuan Y, Long M, Luo T and Bian J: Beclin-1-mediated autophagy protects against cadmium-activated apoptosis via the Fas/FasL pathway in primary rat proximal tubular cell culture. Sci Rep. 8:3941–3948. 2017.

19 

Zhou X, Hong T, Yu Q, Nie S, Gong D, Xiong T and Xie M: Exopolysaccharides from Lactobacillus plantarum NCU116 induce c-Jun dependent Fas/Fasl-mediated apoptosis via TLR2 in mouse intestinal epithelial cancer cells. Sci Rep. 7:142472017. View Article : Google Scholar : PubMed/NCBI

20 

Hu L, Chen L, Yang G, Li L, Sun H, Chang Y, Tu Q, Wu M and Wang H: HBx sensitizes cells to oxidative stress-induced apoptosis by accelerating the loss of Mcl-1 protein via caspase-3 cascade. Mol Cancer. 10:432011. View Article : Google Scholar : PubMed/NCBI

21 

Zhou X and Hong T: Exopolysaccharides from Lactobacillus plantarum NCU116 induce c-Jun dependent Fas/Fasl-mediated apoptosis via TLR2 in mouse intestinal epithelial cancer cells. Retrovirology. 5:14–35. 2008.PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Feng J, Feng L and Zhang G: Mitochondrial damage in hippocampal neurons of rats with epileptic protein expression of Fas and caspase-3. Exp Ther Med 16: 2483-2489, 2018.
APA
Feng, J., Feng, L., & Zhang, G. (2018). Mitochondrial damage in hippocampal neurons of rats with epileptic protein expression of Fas and caspase-3. Experimental and Therapeutic Medicine, 16, 2483-2489. https://doi.org/10.3892/etm.2018.6439
MLA
Feng, J., Feng, L., Zhang, G."Mitochondrial damage in hippocampal neurons of rats with epileptic protein expression of Fas and caspase-3". Experimental and Therapeutic Medicine 16.3 (2018): 2483-2489.
Chicago
Feng, J., Feng, L., Zhang, G."Mitochondrial damage in hippocampal neurons of rats with epileptic protein expression of Fas and caspase-3". Experimental and Therapeutic Medicine 16, no. 3 (2018): 2483-2489. https://doi.org/10.3892/etm.2018.6439
Copy and paste a formatted citation
x
Spandidos Publications style
Feng J, Feng L and Zhang G: Mitochondrial damage in hippocampal neurons of rats with epileptic protein expression of Fas and caspase-3. Exp Ther Med 16: 2483-2489, 2018.
APA
Feng, J., Feng, L., & Zhang, G. (2018). Mitochondrial damage in hippocampal neurons of rats with epileptic protein expression of Fas and caspase-3. Experimental and Therapeutic Medicine, 16, 2483-2489. https://doi.org/10.3892/etm.2018.6439
MLA
Feng, J., Feng, L., Zhang, G."Mitochondrial damage in hippocampal neurons of rats with epileptic protein expression of Fas and caspase-3". Experimental and Therapeutic Medicine 16.3 (2018): 2483-2489.
Chicago
Feng, J., Feng, L., Zhang, G."Mitochondrial damage in hippocampal neurons of rats with epileptic protein expression of Fas and caspase-3". Experimental and Therapeutic Medicine 16, no. 3 (2018): 2483-2489. https://doi.org/10.3892/etm.2018.6439
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team