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Article Open Access

Effects of SB202190 on expression levels of IL-6 and NF-κB in flap ischemia-reperfusion injury

  • Authors:
    • Kai Guo
    • Jingxin Ma
    • Wenyong Liang
  • View Affiliations / Copyright

    Affiliations: Department III of Orthopaedics, Jinan Municipal Hospital of Traditional Chinese Medicine, Jinan, Shandong 250000, P.R. China, Department of Spine Surgery, Jinan Zhangqiu District Hospital of Traditional Chinese Medicine,Jinan, Shandong 250000, P.R. China, Department of Hand, Foot and Ankle Surgery, Jining No. 1 People's Hospital, Jining, Shandong 272011, P.R. China
    Copyright: © Guo et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 2522-2526
    |
    Published online on: July 13, 2018
       https://doi.org/10.3892/etm.2018.6442
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Abstract

The aim of the current study was to investigate the effect of SB202190, a specific inhibitor of p38 MAPK signaling pathway, on the expression levels of IL-6 and NF-κB in flap ischemia-reperfusion injury. Healthy Sprague-Dawley rats were randomly divided into four groups of 12 each. For the ischemia-reperfusion group, the flap was constructed and then sutured after 8 h of ischemia. For the saline group, rats were intraperitoneally infused with saline at regular intervals after flap ischemia-reperfusion. For the inhibitor group, rats were intraperitoneally infused with SB202190 at regular intervals after flap ischemia-reperfusion. For the control group, the flap was constructed and then sutured immediately. The flap survival rate of each group was measured after 7 days. The concentration of IL-6 in serum was measured by ELISA kit. The mRNA and protein expression levels of IL-6 and NF-κB in the flap were measured using RT-PCR and western blot analysis, respectively. In the ischemia-reperfusion group and the saline group, the flap survival rates were much lower than that in the control group (P<0.05). By contrast, the mRNA and protein expression levels of IL-6 and NF-κB in the flap and the concentration of IL-6 in serum were much higher (P<0.05). In the inhibitor group, the flap survival rate was significantly higher than those in the ischemia-reperfusion and saline groups (P<0.05). By contrast, the concentration of IL-6 in serum and the mRNA and protein expression levels of NF-κB and IL-6 in the flap were significantly decreased (P<0.05). The results show that, SB202190 played a role in the protection of the flap by reducing the inflammatory response in flap ischemia-reperfusion injury.
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Copy and paste a formatted citation
Spandidos Publications style
Guo K, Ma J and Liang W: Effects of SB202190 on expression levels of IL-6 and NF-κB in flap ischemia-reperfusion injury. Exp Ther Med 16: 2522-2526, 2018.
APA
Guo, K., Ma, J., & Liang, W. (2018). Effects of SB202190 on expression levels of IL-6 and NF-κB in flap ischemia-reperfusion injury. Experimental and Therapeutic Medicine, 16, 2522-2526. https://doi.org/10.3892/etm.2018.6442
MLA
Guo, K., Ma, J., Liang, W."Effects of SB202190 on expression levels of IL-6 and NF-κB in flap ischemia-reperfusion injury". Experimental and Therapeutic Medicine 16.3 (2018): 2522-2526.
Chicago
Guo, K., Ma, J., Liang, W."Effects of SB202190 on expression levels of IL-6 and NF-κB in flap ischemia-reperfusion injury". Experimental and Therapeutic Medicine 16, no. 3 (2018): 2522-2526. https://doi.org/10.3892/etm.2018.6442
Copy and paste a formatted citation
x
Spandidos Publications style
Guo K, Ma J and Liang W: Effects of SB202190 on expression levels of IL-6 and NF-κB in flap ischemia-reperfusion injury. Exp Ther Med 16: 2522-2526, 2018.
APA
Guo, K., Ma, J., & Liang, W. (2018). Effects of SB202190 on expression levels of IL-6 and NF-κB in flap ischemia-reperfusion injury. Experimental and Therapeutic Medicine, 16, 2522-2526. https://doi.org/10.3892/etm.2018.6442
MLA
Guo, K., Ma, J., Liang, W."Effects of SB202190 on expression levels of IL-6 and NF-κB in flap ischemia-reperfusion injury". Experimental and Therapeutic Medicine 16.3 (2018): 2522-2526.
Chicago
Guo, K., Ma, J., Liang, W."Effects of SB202190 on expression levels of IL-6 and NF-κB in flap ischemia-reperfusion injury". Experimental and Therapeutic Medicine 16, no. 3 (2018): 2522-2526. https://doi.org/10.3892/etm.2018.6442
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