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Long noncoding RNA EZR‑AS1 promotes tumor growth and metastasis by modulating Wnt/β‑catenin pathway in breast cancer

  • Authors:
    • Yu Bai
    • Xian Zhou
    • Luo Huang
    • Yue Wan
    • Xiaoyu Li
    • Ying Wang
  • View Affiliations / Copyright

    Affiliations: Department of Radiation Oncology, Chongqing University Cancer Hospital and Chongqing Cancer Institute and Hospital, Chongqing 400030, P.R. China
    Copyright: © Bai et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 2235-2242
    |
    Published online on: July 18, 2018
       https://doi.org/10.3892/etm.2018.6461
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Abstract

Accumulating evidence has demonstrated that long noncoding RNAs (lncRNAs) serve important roles in tumor development and progression. However, whether lncRNA EZR‑AS1 is associated with breast cancer (BC) progression remains unclear. In the present study, reverse transcription‑quantitative polymerase chain reaction analysis demonstrated that the expression of EZR‑AS1 was significantly upregulated in BC tissues and cell lines. Furthermore, Kaplan‑Meier curve analysis revealed that increased EZR‑AS1 expression in patients with BC contributes to poor prognosis. Cell counting kit‑8 and fluorescence‑activated cell sorting experiments indicated that EZR‑AS1 knockdown significantly suppressed the proliferation and cell cycle progression of breast cancer cells, while reducing cellular apoptosis. Furthermore, Transwell assays suggested that EZR‑AS1 knockdown reduced the migration and invasion ability of BC cells compared with control cells. In the present study, it was observed that EZR‑AS1 interacts with β‑catenin to prevent degradation. EZR‑AS1 knockdown resulted in β‑catenin downregulation and inactivation of the Wnt/β‑catenin pathway. Rescue assays revealed that β‑catenin overexpression reversed the effects of EZR‑AS1 knockdown on BC cell proliferation, apoptosis, migration and invasion. In conclusion, the results of the present study demonstrate that EZR‑AS1 serves as an oncogene in BC via activating the Wnt/β‑catenin pathway. This suggests that EZR‑AS1 may be a therapeutic target for BC treatment.
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1 

Runowicz CD, Leach CR, Henry NL, Henry KS, Mackey HT, Cowens-Alvarado RL, Cannady RS, Pratt-Chapman ML, Edge SB, Jacobs LA, et al: American Cancer Society/American Society of clinical oncology breast cancer survivorship care guideline. J Clin Oncol. 34:611–635. 2016. View Article : Google Scholar : PubMed/NCBI

2 

Hong W and Dong E: The past, present and future of breast cancer research in China. Cancer Lett. 351:1–5. 2014. View Article : Google Scholar : PubMed/NCBI

3 

Zhang Z, Sun L, Zhang Y, Lu G, Li Y and Wei Z: Long non-coding RNA FEZF1-AS1 promotes breast cancer stemness and tumorigenesis via targeting miR-30a/Nanog axis. J Cell Physiol. 2018. View Article : Google Scholar

4 

Sun Y, Zeng C, Gan S, Li H, Cheng Y, Chen D, Li R and Zhu W: LncRNA HOTTIP-mediated HOXA11 expression promotes cell growth, migration and inhibits cell apoptosis in breast cancer. Int J Mol Sci. 19:E4722018. View Article : Google Scholar : PubMed/NCBI

5 

Li Y, Jiang B, Wu X, Huang Q, Chen W, Zhu H, Qu X, Xie L, Ma X and Huang G: Long non-coding RNA MIAT is estrogen-responsive and promotes estrogen-induced proliferation in ER-positive breast cancer cells. Biochem Biophys Res Commun. S0006-291X(18)31215-4. 2018.

6 

Shi D and Zhang Y, Lu R and Zhang Y: The long non-coding RNA MALAT1 interacted with miR-218 modulates choriocarcinoma growth by targeting Fbxw8. Biomed Pharmacother. 97:543–550. 2018. View Article : Google Scholar : PubMed/NCBI

7 

Ye B, Liu B, Yang L, Zhu X, Zhang D, Wu W, Zhu P, Wang Y, Wang S, Xia P, et al: LncKdm2b controls self-renewal of embryonic stem cells via activating expression of transcription factor Zbtb3. EMBO J. 37:e971742018. View Article : Google Scholar : PubMed/NCBI

8 

Caldwell KK, Hafez A, Solomon E, Cunningham M and Allan AM: Arsenic exposure during embryonic development alters the expression of the long noncoding RNA growth arrest specific-5 (Gas5) in a sex-dependent manner. Neurotoxicol Teratol. 66:102–112. 2018. View Article : Google Scholar : PubMed/NCBI

9 

Liu B, Ye B, Yang L, Zhu X, Huang G, Zhu P, Du Y, Wu J, Qin X, Chen R, et al: Long noncoding RNA lncKdm2b is required for ILC3 maintenance by initiation of Zfp292 expression. Nat Immunol. 18:499–508. 2017. View Article : Google Scholar : PubMed/NCBI

10 

Zhu P, Wang Y, Wu J, Huang G, Liu B, Ye B, Du Y, Gao G, Tian Y, He L and Fan Z: LncBRM initiates YAP1 signalling activation to drive self-renewal of liver cancer stem cells. Nat Commun. 7:136082016. View Article : Google Scholar : PubMed/NCBI

11 

Luo Y, Ouyang J, Zhou D, Zhong S, Wen M, Ou W, Yu H, Jia L and Huang Y: Long noncoding RNA GAPLINC promotes cells migration and invasion in colorectal cancer cell by regulating miR-34a/c-MET signal pathway. Dig Dis Sci. 63:890–899. 2018. View Article : Google Scholar : PubMed/NCBI

12 

Chen C, Zhou L, Wang H, Chen J, Li W, Liu W, Shen M, Liu H and Fu X: Long noncoding RNA CNALPTC1 promotes cell proliferation and migration of papillary thyroid cancer via sponging miR-30 family. Am J Cancer Res. 8:192–206. 2018.PubMed/NCBI

13 

Wang Y, Zhang W, Wang Y and Wang S: HOXD-AS1 promotes cell proliferation, migration and invasion through miR-608/FZD4 axis in ovarian cancer. Am J Cancer Res. 8:170–182. 2018.PubMed/NCBI

14 

Pan L, Liang W, Gu J, Zang X, Huang Z, Shi H, Chen J, Fu M, Zhang P, Xiao X, et al: Long noncoding RNA DANCR is activated by SALL4 and promotes the proliferation and invasion of gastric cancer cells. Oncotarget. 9:1915–1930. 2018. View Article : Google Scholar : PubMed/NCBI

15 

Tang R, Wu JC, Zheng LM, Li ZR, Zhou KL, Zhang ZS, Xu DF and Chen C: Long noncoding RNA RUSC1-AS-N indicates poor prognosis and increases cell viability in hepatocellular carcinoma. Eur Rev Med Pharmacol Sci. 22:388–396. 2018.PubMed/NCBI

16 

Zhang XD, Huang GW, Xie YH, He JZ, Guo JC, Xu XE, Liao LD, Xie YM, Song YM, Li EM and Xu LY: The interaction of lncRNA EZR-AS1 with SMYD3 maintains overexpression of EZR in ESCC cells. Nucleic Acids Res. 46:1793–1809. 2018. View Article : Google Scholar : PubMed/NCBI

17 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI

18 

Luo X, Qiu Y, Jiang Y, Chen F, Jiang L, Zhou Y, Dan H, Zeng X, Lei YL and Chen Q: Long non-coding RNA implicated in the invasion and metastasis of head and neck cancer: Possible function and mechanisms. Mol Cancer. 17:142018. View Article : Google Scholar : PubMed/NCBI

19 

Zhou S, Wang L, Yang Q, Liu H, Meng Q, Jiang L, Wang S and Jiang W: Systematical analysis of lncRNA-mRNA competing endogenous RNA network in breast cancer subtypes. Breast Cancer Res Treat. 169:267–275. 2018. View Article : Google Scholar : PubMed/NCBI

20 

Zhang YX, Yuan J, Gao ZM and Zhang ZG: LncRNA TUC338 promotes invasion of lung cancer by activating MAPK pathway. Eur Rev Med Pharmacol Sci. 22:443–449. 2018.PubMed/NCBI

21 

Peng R, Luo C, Guo Q, Cao J, Yang Q, Dong K, Wang S, Wang K and Song C: Association analyses of genetic variants in long non-coding RNA MALAT1 with breast cancer susceptibility and mRNA expression of MALAT1 in Chinese Han population. Gene. 642:241–248. 2018. View Article : Google Scholar : PubMed/NCBI

22 

Liu G, Hu X and Zhou G: Long non-coding RNA OR3A4 promotes proliferation and migration in breast cancer. Biomed Pharmacother. 96:426–433. 2017. View Article : Google Scholar : PubMed/NCBI

23 

Lai XJ and Cheng HF: LncRNA colon cancer-associated transcript 1 (CCAT1) promotes proliferation and metastasis of ovarian cancer via miR-1290. Eur Rev Med Pharmacol Sci. 22:322–328. 2018.PubMed/NCBI

24 

Xu W, He L, Li Y, Tan Y, Zhang F and Xu H: Silencing of lncRNA ZFAS1 inhibits malignancies by blocking Wnt/β-catenin signaling in gastric cancer cells. Biosci Biotechnol Biochem. 82:456–465. 2018. View Article : Google Scholar : PubMed/NCBI

25 

Zhang P, Zhou H, Lu K, Lu Y, Wang Y and Feng T: Exosome-mediated delivery of MALAT1 induces cell proliferation in breast cancer. Onco Targets Ther. 11:291–299. 2018. View Article : Google Scholar : PubMed/NCBI

26 

Zhang S, Wang J, Ghoshal T, Wilkins D, Mo YY, Chen Y and Zhou Y: lncRNA gene signatures for prediction of breast cancer intrinsic subtypes and prognosis. Genes (Basel). 9:E652018. View Article : Google Scholar : PubMed/NCBI

27 

Li Z, Hou P, Fan D, Dong M, Ma M, Li H, Yao R, Li Y, Wang G, Geng P, et al: The degradation of EZH2 mediated by lncRNA ANCR attenuated the invasion and metastasis of breast cancer. Cell Death Differ. 24:59–71. 2017. View Article : Google Scholar : PubMed/NCBI

28 

Huang W, Cui X, Chen J, Feng Y, Song E, Li J and Liu Y: Long non-coding RNA NKILA inhibits migration and invasion of tongue squamous cell carcinoma cells via suppressing epithelial-mesenchymal transition. Oncotarget. 7:62520–62532. 2016.PubMed/NCBI

29 

Xu YC, Liang CJ, Zhang DX, Li GQ, Gao X, Fu JZ, Xia F, Ji JJ, Zhang LJ, Li GM and Wu JX: LncSHRG promotes hepatocellular carcinoma progression by activating HES6. Oncotarget. 8:70630–70641. 2017.PubMed/NCBI

30 

Shi J, Wang YJ, Sun CR, Qin B, Zhang Y and Chen G: Long noncoding RNA lncHERG promotes cell proliferation, migration and invasion in glioblastoma. Oncotarget. 8:108031–108041. 2017. View Article : Google Scholar : PubMed/NCBI

31 

Zhu P, Wang Y, Huang G, Ye B, Liu B, Wu J, Du Y, He L and Fan Z: lnc-β-Catm elicits EZH2-dependent β-catenin stabilization and sustains liver CSC self-renewal. Nat Struct Mol Biol. 23:631–639. 2016. View Article : Google Scholar : PubMed/NCBI

32 

Ouyang S, Zheng X, Zhou X, Chen Z, Yang X and Xie M: LncRNA BCAR4 promotes colon cancer progression via activating Wnt/β-catenin signaling. Oncotarget. 8:92815–92826. 2017. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Bai Y, Zhou X, Huang L, Wan Y, Li X and Wang Y: Long noncoding RNA EZR‑AS1 promotes tumor growth and metastasis by modulating Wnt/β‑catenin pathway in breast cancer. Exp Ther Med 16: 2235-2242, 2018.
APA
Bai, Y., Zhou, X., Huang, L., Wan, Y., Li, X., & Wang, Y. (2018). Long noncoding RNA EZR‑AS1 promotes tumor growth and metastasis by modulating Wnt/β‑catenin pathway in breast cancer. Experimental and Therapeutic Medicine, 16, 2235-2242. https://doi.org/10.3892/etm.2018.6461
MLA
Bai, Y., Zhou, X., Huang, L., Wan, Y., Li, X., Wang, Y."Long noncoding RNA EZR‑AS1 promotes tumor growth and metastasis by modulating Wnt/β‑catenin pathway in breast cancer". Experimental and Therapeutic Medicine 16.3 (2018): 2235-2242.
Chicago
Bai, Y., Zhou, X., Huang, L., Wan, Y., Li, X., Wang, Y."Long noncoding RNA EZR‑AS1 promotes tumor growth and metastasis by modulating Wnt/β‑catenin pathway in breast cancer". Experimental and Therapeutic Medicine 16, no. 3 (2018): 2235-2242. https://doi.org/10.3892/etm.2018.6461
Copy and paste a formatted citation
x
Spandidos Publications style
Bai Y, Zhou X, Huang L, Wan Y, Li X and Wang Y: Long noncoding RNA EZR‑AS1 promotes tumor growth and metastasis by modulating Wnt/β‑catenin pathway in breast cancer. Exp Ther Med 16: 2235-2242, 2018.
APA
Bai, Y., Zhou, X., Huang, L., Wan, Y., Li, X., & Wang, Y. (2018). Long noncoding RNA EZR‑AS1 promotes tumor growth and metastasis by modulating Wnt/β‑catenin pathway in breast cancer. Experimental and Therapeutic Medicine, 16, 2235-2242. https://doi.org/10.3892/etm.2018.6461
MLA
Bai, Y., Zhou, X., Huang, L., Wan, Y., Li, X., Wang, Y."Long noncoding RNA EZR‑AS1 promotes tumor growth and metastasis by modulating Wnt/β‑catenin pathway in breast cancer". Experimental and Therapeutic Medicine 16.3 (2018): 2235-2242.
Chicago
Bai, Y., Zhou, X., Huang, L., Wan, Y., Li, X., Wang, Y."Long noncoding RNA EZR‑AS1 promotes tumor growth and metastasis by modulating Wnt/β‑catenin pathway in breast cancer". Experimental and Therapeutic Medicine 16, no. 3 (2018): 2235-2242. https://doi.org/10.3892/etm.2018.6461
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