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Article Open Access

Osteogenic potential of cell spheroids composed of varying ratios of gingiva‑derived and bone marrow stem cells using concave microwells

  • Authors:
    • Jae‑Yong Tae
    • Hyuna Lee
    • Hyunjin Lee
    • Youngkyung Ko
    • Jun‑Beom Park
  • View Affiliations / Copyright

    Affiliations: Department of Periodontics, College of Medicine, The Catholic University of Korea, Seoul 06591, Republic of Korea
    Copyright: © Tae et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 2287-2294
    |
    Published online on: July 18, 2018
       https://doi.org/10.3892/etm.2018.6462
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Abstract

The aim of the current study was to evaluate cell viability and osteogenic differentiation potential in cell spheroids composed of varying ratios of gingiva‑derived and bone marrow stem cells cultured in concave microwells. Cell spheroids were established from bone marrow and gingiva‑derived stem cells in ratios of 6:0 (Group 1), 2:1 (Group 2), 3:3 (Group 3), 1:2 (Group 4), and 0:6 (Group 5). On days 3 and 5, the viability of the cell spheroids was qualitatively analyzed using a calcein acetoxymethyl ester working solution and an ethidium homodimer‑1 live/dead assay. On days 1, 3, 5 and 7, a quantitative cell viability analysis was performed using a Cell Counting Kit‑8. Alkaline phosphatase activity assays were performed using a commercially available kit on day 7 to assess osteogenic differentiation. In addition, reverse transcription‑quantitative polymerase chain reaction and western blot analysis were performed to evaluate runt‑related transcription factor 2 (Runx2) and osteocalcin expression. The ratio of gingiva‑derived to bone marrow stem cells did not affect the stem cell spheroid morphology. No significant changes in cell viability were noted among the different groups following incubation for 7 days. A consistent alkaline phosphatase activity was measured in co‑cultured gingiva‑derived and bone marrow stem cell spheroids of varying compositions. Runx2 and osteocalcin expression was increased when co‑cultured compared with pure gingiva‑derived or bone marrow stem cells. In conclusion, stem cell spheroids established by co‑culturing maintained morphology, viability and a high osteogenic differentiation potential during the experimental period of 7 days. These spheroids containing human gingiva‑derived and bone marrow stem cells may enhance the osteogenic differentiation potential. The use of multicell spheroids may be a simple and effective strategy for improving stem cell therapy.
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Copy and paste a formatted citation
Spandidos Publications style
Tae JY, Lee H, Lee H, Ko Y and Park JB: Osteogenic potential of cell spheroids composed of varying ratios of gingiva‑derived and bone marrow stem cells using concave microwells. Exp Ther Med 16: 2287-2294, 2018.
APA
Tae, J., Lee, H., Lee, H., Ko, Y., & Park, J. (2018). Osteogenic potential of cell spheroids composed of varying ratios of gingiva‑derived and bone marrow stem cells using concave microwells. Experimental and Therapeutic Medicine, 16, 2287-2294. https://doi.org/10.3892/etm.2018.6462
MLA
Tae, J., Lee, H., Lee, H., Ko, Y., Park, J."Osteogenic potential of cell spheroids composed of varying ratios of gingiva‑derived and bone marrow stem cells using concave microwells". Experimental and Therapeutic Medicine 16.3 (2018): 2287-2294.
Chicago
Tae, J., Lee, H., Lee, H., Ko, Y., Park, J."Osteogenic potential of cell spheroids composed of varying ratios of gingiva‑derived and bone marrow stem cells using concave microwells". Experimental and Therapeutic Medicine 16, no. 3 (2018): 2287-2294. https://doi.org/10.3892/etm.2018.6462
Copy and paste a formatted citation
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Spandidos Publications style
Tae JY, Lee H, Lee H, Ko Y and Park JB: Osteogenic potential of cell spheroids composed of varying ratios of gingiva‑derived and bone marrow stem cells using concave microwells. Exp Ther Med 16: 2287-2294, 2018.
APA
Tae, J., Lee, H., Lee, H., Ko, Y., & Park, J. (2018). Osteogenic potential of cell spheroids composed of varying ratios of gingiva‑derived and bone marrow stem cells using concave microwells. Experimental and Therapeutic Medicine, 16, 2287-2294. https://doi.org/10.3892/etm.2018.6462
MLA
Tae, J., Lee, H., Lee, H., Ko, Y., Park, J."Osteogenic potential of cell spheroids composed of varying ratios of gingiva‑derived and bone marrow stem cells using concave microwells". Experimental and Therapeutic Medicine 16.3 (2018): 2287-2294.
Chicago
Tae, J., Lee, H., Lee, H., Ko, Y., Park, J."Osteogenic potential of cell spheroids composed of varying ratios of gingiva‑derived and bone marrow stem cells using concave microwells". Experimental and Therapeutic Medicine 16, no. 3 (2018): 2287-2294. https://doi.org/10.3892/etm.2018.6462
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