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Effects of hepatic blood inflow on liver ultrastructure and regeneration after extensive liver resection in rats with cirrhosis

  • Authors:
    • Wang‑Xun Jin
    • Bing Wang
    • Yun‑Li Zhang
    • Ruizeng Dong
    • Xin‑Bao Wang
    • Jian‑Min Guo
    • Sunfu Fan
    • Bingqi Yu
  • View Affiliations / Copyright

    Affiliations: Department of Abdominal Surgery, Zhejiang Cancer Hospital, Hangzhou, Zhejiang 330022, P.R. China
    Copyright: © Jin et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 2573-2583
    |
    Published online on: July 18, 2018
       https://doi.org/10.3892/etm.2018.6467
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Abstract

The aim of the present study was to investigate the effects of hepatic blood inflow on liver function, liver ultrastructure and the regeneration of future liver remnant (FLR) following major hepatectomy in rats with liver cirrhosis. A rat model of cirrhosis was established through intraperitoneal injection of carbon tetrachloride for 8 consecutive weeks. Extensive liver resection and different blood inflow models by portal vein (PV) and/or hepatic artery (HA) stenosis were conducted on the cirrhosis rats. Animal models were constructed as follows: Control (group A), low‑flow PV + high‑flow HA (group B), low‑flow PV + low‑flow HA (group C), high‑flow PV + high‑flow HA (group D) and high‑flow PV + low‑flow HA (group E). Hepatic blood inflow was detected by laser speckle contrast analysis, liver function and pathological changes were analyzed, Masson staining was used to identify the fibrosis of the liver and Periodic acid‑Schiff staining was used to identify glycogen synthesis and hepatocyte function. The liver cell ultrastructure was evaluated by transmission electron microscopy, and the expression of Ki‑67 in hepatocytes and the weight of the FLR were recorded to determine the regeneration of the FLR. Five days after major hepatectomy and liver blood inflow modulation, pathological examination of the livers from groups B and C revealed less congestion and less extensive hepatocellular injury. The serum alanine aminotransferase level of group B at 1, 3 and 5 days after hepatectomy and blood inflow modulation was 460.9±31.7, 331.0±22.0 and 285.6±15.8 U/l, respectively (control group: 676.9±41.7, 574.9±28.0 and 436.1±32.7 U/l, respectively; P<0.05); the total bilirubin of group B at 1, 3 and 5 days was 20.4±1.5, 16.1±1.0 and 13.5±0.6 µmol/l, respectively (control group: 30.3±1.4, 26.5±0.8 and 22.1±1.2 µmol/l, respectively; P<0.05). The size of the endoplasmic reticulum in the low‑flow PV groups increased significantly and the mitochondrial swelling was alleviated. The positive rate of Ki‑67 in the hepatocytes of groups B, C and D was 23.9±3.6, 15.7±2.3 and 12.9±2.4%, respectively (control group: 10.1±2.1%, P<0.05), and the positive rate of Ki‑67 in group E was 6.1±1.4% (compared with that of the control group, P<0.05). The remnant liver weight of group B was 15.4±1.0 g (compared with that of the control group, P<0.05). Therefore, decreased portal blood flow combined with increased hepatic arterial blood flow alleviated the congestion in the liver following major hepatectomy in cirrhotic rats, improved the pathological status and liver function, increased the expression of Ki‑67 and promoted liver regeneration.
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View References

1 

Poon RT, Fan ST, Lo CM, Liu CL, Lam CM, Yuen WK, Yeung C and Wong J: Improving perioperative outcome expands the role of hepatectomy in management of benign and malignant hepatobiliary diseases: Analysis of 1222 consecutive patients from a prospective database. Ann Surg. 240:698–710. 2004.PubMed/NCBI

2 

Fondevila C, Hessheimer AJ, Taurá P, Sánchez O, Calatayud D, de Riva N, Muñoz J, Fuster J, Rimola A and García-Valdecasas JC: Portal hyperperfusion: Mechanism of injury and stimulus for regeneration in porcine small-for-size transplantation. Liver Transpl. 16:364–374. 2010. View Article : Google Scholar : PubMed/NCBI

3 

Kinaci E and Kayaalp C: Portosystemic shunts for ‘too small-for-size syndrome’ after liver transplantation: A systematic review. World J Surg. 40:1932–1940. 2016. View Article : Google Scholar : PubMed/NCBI

4 

Xiang L, Huang L, Wang X, Zhao Y, Liu Y and Tan J: How much portal vein flow is too much for liver remnant in a stable porcine model? Transplant Proc. 48:234–241. 2016. View Article : Google Scholar : PubMed/NCBI

5 

Rahbari NN, Garden OJ, Padbury R, Brooke-Smith M, Crawford M, Adam R, Koch M, Makuuchi M, Dematteo RP, Christophi C, et al: Posthepatectomy liver failure: A definition and grading by the international study group of liver surgery (ISGLS). Surgery. 149:713–724. 2011. View Article : Google Scholar : PubMed/NCBI

6 

Yadav K, Shrikhande S and Goel M: Post hepatectomy liver failure: Concept of management. J Gastrointest Cancer. 45:405–513. 2014. View Article : Google Scholar : PubMed/NCBI

7 

Li CH, Chen YW, Chen YL, Yao LB, Ge XL, Pan K, Zhang AQ and Dong JH: Preserving low perfusion during surgical liver blood inflow control prevents hepatic microcirculatory dysfunction and irreversible hepatocyte injury in rats. Sci Rep. 5:144062015. View Article : Google Scholar : PubMed/NCBI

8 

Liu C, Song JL, Lu WS, Yang JY, Jiang L, Yan LN, Zhang JY, Lu Q, Wen TF, Xu MQ and Wang WT: Hepatic arterial buffer response maintains the homeostasis of graft hemodynamics in patient receiving living donor liver transplantation. Dig Dis Sci. 61:464–473. 2016. View Article : Google Scholar : PubMed/NCBI

9 

Audebert C, Bekheit M, Bucur P, Vibert E and Vignon-Clementel IE: Partial hepatectomy hemodynamics changes: Experimental data explained by closed-loop lumped modeling. J Biomech. 50:202–208. 2017. View Article : Google Scholar : PubMed/NCBI

10 

Andreou A, Vauthey JN, Cherqui D, Zimmitti G, Ribero D, Truty MJ, Wei SH, Curley SA, Laurent A, Poon RT, et al: Improved long-term survival after major resection for hepatocellular carcinoma: A multicenter analysis based on a new definition of major hepatectomy. J Gastrointest Surg. 17:66–77; discussion p.77. 2013. View Article : Google Scholar : PubMed/NCBI

11 

Li CH, Ge XL, Pan K, Wang PF, Su YN and Zhang AQ: Laser speckle contrast imaging and oxygen to see for assessing microcirculatory liver blood flow changes following different volumes of hepatectomy. Microvasc Res. 110:14–23. 2017. View Article : Google Scholar : PubMed/NCBI

12 

Etra JW, Squires MH III, Fisher SB, Rutz DR, Martin BM, Kooby DA, Cardona K, Sarmiento JM, Staley CA III, Maithel SK and Russell MC: Early identification of patients at increased risk for hepatic insufficiency, complications and mortality after major hepatectomy. HPB (Oxford). 16:875–883. 2014. View Article : Google Scholar : PubMed/NCBI

13 

Dold S, Richter S, Kollmar O, von Heesen M, Scheuer C, Laschke MW, Vollmar B, Schilling MK and Menger MD: Portal hyperperfusion after extended hepatectomy does not induce a hepatic arterial buffer response (HABR) but impairs mitochondrial redox state and hepatocellular oxygenation. PLoS One. 10:e01418772015. View Article : Google Scholar : PubMed/NCBI

14 

Bucur PO, Bekheit M, Audebert C, Othman A, Hammad S, Sebagh M, Allard MA, Decante B, Friebel A, Miquelestorena-Standley E, et al: Modulating portal hemodynamics with vascular ring allows efficient regeneration after partial hepatectomy in a porcine model. Ann Surg. 268:134–142. 2018.PubMed/NCBI

15 

Schleimer K, Stippel DL, Kasper HU, Tawadros S, Allwissner R, Gaudig C, Greiner T, Hölscher AH and Beckurts KT: Portal hyperperfusion causes disturbance of microcirculation and increased rate of hepatocellular apoptosis: Investigations in heterotopic rat liver transplantation with portal vein arterialization. Transplant Proc. 38:725–729. 2006. View Article : Google Scholar : PubMed/NCBI

16 

Alexandrino H, Rolo A, Teodoro JS, Donato H, Martins R, Serôdio M, Martins M, Tralhão JG, Caseiro Alves F, Palmeira C and Castro E Sousa F: Bioenergetic adaptations of the human liver in the ALPPS procedure - how liver regeneration correlates with mitochondrial energy status. HPB (Oxford). 19:1091–1103. 2017. View Article : Google Scholar : PubMed/NCBI

17 

Satoh S, Tanaka A, Hatano E, Inomoto T, Iwata S, Kitai T, Shinohara H, Tsunekawa S, Chance B and Yamaoka Y: Energy metabolism and regeneration in transgenic mouse liver expressing creatine kinase after major hepatectomy. Gastroenterology. 110:1166–1174. 1996. View Article : Google Scholar : PubMed/NCBI

18 

Koyama H, Kurokawa T, Nonami T, Nakao A, Sugiyama S, Murakami T, Shimomura Y and Takagi H: Increases in the mitochondrial DNA replication and transcription in the remnant liver of rats. Biochem Biophys Res Commun. 243:858–861. 1998. View Article : Google Scholar : PubMed/NCBI

19 

Alexandrino H, Varela AT, Teodoro JS, Martins MA, Rolo AP, Tralhão JG, Palmeira CM, Castro E and Sousa F: Mitochondrial bioenergetics and posthepatectomy liver dysfunction. Eur J Clin Invest. 46:627–635. 2016. View Article : Google Scholar : PubMed/NCBI

20 

Weng J, Li W, Jia X and An W: Alleviation of ischemia-reperfusion injury in liver steatosis by augmenter of liver regeneration is attributed to antioxidation and preservation of mitochondria. Transplantation. 101:2340–2348. 2017. View Article : Google Scholar : PubMed/NCBI

21 

Xing XK, Li MH, Guo ZW and Xu CS: Expression profiles of genes associated with mitochondria-mediated apoptosis and their roles in liver regeneration. Genet Mol Res. 15:2016. View Article : Google Scholar

22 

Fujiyoshi M and Ozaki M: Molecular mechanisms of liver regeneration and protection for treatment of liver dysfunction and diseases. J Hepatobiliary Pancreat Sci. 18:13–22. 2011. View Article : Google Scholar : PubMed/NCBI

23 

Tao Y, Wang M, Chen E and Tang H: Liver regeneration: Analysis of the main relevant signaling molecules. Mediators Inflamm. 2017:42563522017. View Article : Google Scholar : PubMed/NCBI

24 

Xia J, Zhou Y, Ji H, Wang Y, Wu Q, Bao J, Ye F, Shi Y and Bu H: Loss of histone deacetylases 1 and 2 in hepatocytes impairs murine liver regeneration through Ki67 depletion. Hepatology. 58:2089–2098. 2013. View Article : Google Scholar : PubMed/NCBI

25 

Troisi RI, Berardi G, Tomassini F and Sainz-Barriga M: Graft inflow modulation in adult-to-adult living donor liver transplantation: A systematic review. Transplant Rev (Orlando). 31:127–135. 2017. View Article : Google Scholar : PubMed/NCBI

26 

Baccarani U, Pravisani R, Luigi Adani G, Lorenzin D, Cherchi V, Toniutto P and Risaliti A: Safety and efficacy of splenic artery embolization for portal hyperperfusion in liver transplant recipients: A 5-year experience. Liver Transpl. 21:1457–1458. 2015. View Article : Google Scholar : PubMed/NCBI

27 

Eipel C, Abshagen K, Ritter J, Cantré D, Menger MD and Vollmar B: Splenectomy improves survival by increasing arterial blood supply in a rat model of reduced-size liver. Transpl Int. 23:998–1007. 2010. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Jin WX, Wang B, Zhang YL, Dong R, Wang XB, Guo JM, Fan S and Yu B: Effects of hepatic blood inflow on liver ultrastructure and regeneration after extensive liver resection in rats with cirrhosis. Exp Ther Med 16: 2573-2583, 2018.
APA
Jin, W., Wang, B., Zhang, Y., Dong, R., Wang, X., Guo, J. ... Yu, B. (2018). Effects of hepatic blood inflow on liver ultrastructure and regeneration after extensive liver resection in rats with cirrhosis. Experimental and Therapeutic Medicine, 16, 2573-2583. https://doi.org/10.3892/etm.2018.6467
MLA
Jin, W., Wang, B., Zhang, Y., Dong, R., Wang, X., Guo, J., Fan , S., Yu, B."Effects of hepatic blood inflow on liver ultrastructure and regeneration after extensive liver resection in rats with cirrhosis". Experimental and Therapeutic Medicine 16.3 (2018): 2573-2583.
Chicago
Jin, W., Wang, B., Zhang, Y., Dong, R., Wang, X., Guo, J., Fan , S., Yu, B."Effects of hepatic blood inflow on liver ultrastructure and regeneration after extensive liver resection in rats with cirrhosis". Experimental and Therapeutic Medicine 16, no. 3 (2018): 2573-2583. https://doi.org/10.3892/etm.2018.6467
Copy and paste a formatted citation
x
Spandidos Publications style
Jin WX, Wang B, Zhang YL, Dong R, Wang XB, Guo JM, Fan S and Yu B: Effects of hepatic blood inflow on liver ultrastructure and regeneration after extensive liver resection in rats with cirrhosis. Exp Ther Med 16: 2573-2583, 2018.
APA
Jin, W., Wang, B., Zhang, Y., Dong, R., Wang, X., Guo, J. ... Yu, B. (2018). Effects of hepatic blood inflow on liver ultrastructure and regeneration after extensive liver resection in rats with cirrhosis. Experimental and Therapeutic Medicine, 16, 2573-2583. https://doi.org/10.3892/etm.2018.6467
MLA
Jin, W., Wang, B., Zhang, Y., Dong, R., Wang, X., Guo, J., Fan , S., Yu, B."Effects of hepatic blood inflow on liver ultrastructure and regeneration after extensive liver resection in rats with cirrhosis". Experimental and Therapeutic Medicine 16.3 (2018): 2573-2583.
Chicago
Jin, W., Wang, B., Zhang, Y., Dong, R., Wang, X., Guo, J., Fan , S., Yu, B."Effects of hepatic blood inflow on liver ultrastructure and regeneration after extensive liver resection in rats with cirrhosis". Experimental and Therapeutic Medicine 16, no. 3 (2018): 2573-2583. https://doi.org/10.3892/etm.2018.6467
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