Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Experimental and Therapeutic Medicine
Join Editorial Board Propose a Special Issue
Print ISSN: 1792-0981 Online ISSN: 1792-1015
Journal Cover
September-2018 Volume 16 Issue 3

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
September-2018 Volume 16 Issue 3

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article Open Access

Immunomagnetic enrichment to evaluate the role of home environment specimens in transmission of enterovirus 71

  • Authors:
    • Wangfeng Zhu
    • Xiuhui Yang
    • Yong Zhou
    • Yansheng Yan
  • View Affiliations / Copyright

    Affiliations: The School of Public Health, Fujian Medical University, Fuzhou, Fujian 350004, P.R. China, Fujian Province Key Laboratory of Zoonosis Research, Fujian Center for Disease Control and Prevention, Fuzhou, Fujian 350001, P.R. China
    Copyright: © Zhu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 2355-2362
    |
    Published online on: July 19, 2018
       https://doi.org/10.3892/etm.2018.6484
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

Enterovirus 71 (EV71) immunomagnetic enrichment technique and routine detection methods were combined to detect swab environmental specimens to elucidate the role of environmental specimens in the spread of EV71. Immunomagnetic beads with specific enrichment of EV71 virus were prepared, then the beads were used to absorb the EV71 virus from environmental samples. Obtained immunomagnetic bead-virus complexes were detected by RT-PCR, RT-qPCR and cell culture. Isolated virus were subjected to VP1 full-length amplification and homology analysis was performed. A total of 4 µg of EV71 monoclonal antibody was mixed with 50 µl magnetic beads, and the highest coating efficiency was reached after incubating at room temperature for 2 h. Satisfactory enrichment effect was achieved by adding 50 µl immunomagnetic beads to 1.5 ml sample and shaking at room temperature for 2 h. The method of EV71 enrichment has high sensitivity and specificity. A total of 346 specimens after enrichment by immunomagnetic beads, the positive rates of RT-qPCR, RT-PCR and cell culture were 20.52, 5.78, and 9.25%, respectively, which were also significantly higher than those before enrichment (15.90, 3.47 and 4.05%; P<0.05). After enrichment with immunomagnetic beads, isolation rate of EV71 virus from case specimens and home environment specimens increased from 27.45 to 43.14% and from 0 to 5.29%, respectively. In home environment-positive specimens, positive rate of toys and stationery was high (52.00 and 24.00%, respectively). In kindergarten environmental samples, the positive rate of RT-qPCR was 6.12%, and EV71 virus was not isolated. Sequence analysis showed that the nucleotide homology of case isolates and home environment isolates was 98.0-100%.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

View References

1 

Ooi MH, Wong SC, Lewthwaite P, Cardosa MJ and Solomon T: Clinical features, diagnosis, and management of enterovirus 71. Lancet Neurol. 9:1097–1105. 2010. View Article : Google Scholar : PubMed/NCBI

2 

Yi EJ, Shin YJ, Kim JH, Kim TG and Chang SY: Enterovirus 71 infection and vaccines. Clin Exp Vaccine Res. 6:4–14. 2017. View Article : Google Scholar : PubMed/NCBI

3 

Ho M, Chen ER, Hsu KH, Twu SJ, Chen KT, Tsai SF, Wang JR and Shih SR: Taiwan Enterovirus Epidemic Working Group: An epidemic of enterovirus 71 infection in Taiwan. N Engl J Med. 341:929–935. 1999. View Article : Google Scholar : PubMed/NCBI

4 

Herrero LJ, Lee CS, Hurrelbrink RJ, Chua BH, Chua KB and McMinn PC: Molecular epidemiology of enterovirus 71 in peninsular Malaysia, 1997–2000. Arch Virol. 148:1369–1385. 2003. View Article : Google Scholar : PubMed/NCBI

5 

Chan KP, Goh KT, Chong CY, Teo ES, Lau G and Ling AE: Epidemic hand, foot and mouth disease caused by human enterovirus 71, Singapore. Emerg Infect Dis. 9:78–85. 2003. View Article : Google Scholar : PubMed/NCBI

6 

Zhang Y, Tan XJ, Wang HY, Yan DM, Zhu SL, Wang DY, Ji F, Wang XJ, Gao YJ, Chen L, et al: An outbreak of hand, foot, and mouth disease associated with subgenotype C4 of human enterovirus 71 in Shandong, China. J Clin Virol. 44:262–267. 2009. View Article : Google Scholar : PubMed/NCBI

7 

Yang F, Ren L, Xiong Z, Li J, Xiao Y, Zhao R, He Y, Bu G, Zhou S, Wang J, et al: Enterovirus 71 outbreak in the People's Republic of China in 2008. J Clin Microbiol. 47:2351–2352. 2009. View Article : Google Scholar : PubMed/NCBI

8 

Xing W, Liao Q, Viboud C, Zhang J, Sun J, Wu JT, Chang Z, Liu F, Fang VJ, Zheng Y, et al: Hand, foot, and mouth disease in China, 2008–12: An epidemiological study. Lancet Infect Dis. 14:308–318. 2014. View Article : Google Scholar : PubMed/NCBI

9 

Park SK, Park B, Ki M, Kim H, Lee K, Jung C, Sohn YM, Choi SM, Kim DK, Lee DS, et al: Transmission of seasonal outbreak of childhood enteroviral aseptic meningitis and hand-foot-mouth disease. J Korean Med Sci. 25:677–683. 2010. View Article : Google Scholar : PubMed/NCBI

10 

Goh KT, Doraisingham S, Tan JL, Lim GN and Chew SE: An outbreak of hand, foot, and mouth disease in Singapore. Bull World Health Organ. 60:965–969. 1982.PubMed/NCBI

11 

Li P, Li T, Gu Q, Chen X, Li J, Chen X, Chen Y, Zhang D, Gao R, He Z, et al: Children's caregivers and public playgrounds: Potential reservoirs of infection of hand-foot-and-mouth disease. Sci Rep. 6:363752016. View Article : Google Scholar : PubMed/NCBI

12 

Casas N and Suñén E: Detection of enteroviruses, hepatitis A virus and rotaviruses in sewage by means of an immunomagnetic capture reverse transcription-PCR assay. Microbiol Res. 157:169–175. 2002. View Article : Google Scholar : PubMed/NCBI

13 

Liu P, Kim M, Schlesinger D, Kranz C, Ha S, Ha J, Slauch J, Baek S and Moe C: Immunomagnetic separation combined with RT-qPCR for determining the efficacy of disinfectants against human noroviruses. J Infect Public Health. 8:145–154. 2015. View Article : Google Scholar : PubMed/NCBI

14 

Kobayashi S, Natori K, Takeda N and Sakae K: Immunomagnetic capture RT-PCR for detection of norovirus from foods implicated in a foodborne outbreak. Microbiol Immunol. 48:201–204. 2004. View Article : Google Scholar : PubMed/NCBI

15 

Ooi MH, Solomon T, Podin Y, Mohan A, Akin W, Yusuf MA, del Sel S, Kontol KM, Lai BF, Clear D, et al: Evaluation of different clinical sample types in diagnosis of human enterovirus 71-associated hand-foot-and-mouth disease. J Clin Microbiol. 45:1858–1866. 2007. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Zhu W, Yang X, Zhou Y and Yan Y: Immunomagnetic enrichment to evaluate the role of home environment specimens in transmission of enterovirus 71. Exp Ther Med 16: 2355-2362, 2018.
APA
Zhu, W., Yang, X., Zhou, Y., & Yan, Y. (2018). Immunomagnetic enrichment to evaluate the role of home environment specimens in transmission of enterovirus 71. Experimental and Therapeutic Medicine, 16, 2355-2362. https://doi.org/10.3892/etm.2018.6484
MLA
Zhu, W., Yang, X., Zhou, Y., Yan, Y."Immunomagnetic enrichment to evaluate the role of home environment specimens in transmission of enterovirus 71". Experimental and Therapeutic Medicine 16.3 (2018): 2355-2362.
Chicago
Zhu, W., Yang, X., Zhou, Y., Yan, Y."Immunomagnetic enrichment to evaluate the role of home environment specimens in transmission of enterovirus 71". Experimental and Therapeutic Medicine 16, no. 3 (2018): 2355-2362. https://doi.org/10.3892/etm.2018.6484
Copy and paste a formatted citation
x
Spandidos Publications style
Zhu W, Yang X, Zhou Y and Yan Y: Immunomagnetic enrichment to evaluate the role of home environment specimens in transmission of enterovirus 71. Exp Ther Med 16: 2355-2362, 2018.
APA
Zhu, W., Yang, X., Zhou, Y., & Yan, Y. (2018). Immunomagnetic enrichment to evaluate the role of home environment specimens in transmission of enterovirus 71. Experimental and Therapeutic Medicine, 16, 2355-2362. https://doi.org/10.3892/etm.2018.6484
MLA
Zhu, W., Yang, X., Zhou, Y., Yan, Y."Immunomagnetic enrichment to evaluate the role of home environment specimens in transmission of enterovirus 71". Experimental and Therapeutic Medicine 16.3 (2018): 2355-2362.
Chicago
Zhu, W., Yang, X., Zhou, Y., Yan, Y."Immunomagnetic enrichment to evaluate the role of home environment specimens in transmission of enterovirus 71". Experimental and Therapeutic Medicine 16, no. 3 (2018): 2355-2362. https://doi.org/10.3892/etm.2018.6484
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team