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Altered circulating T follicular helper cells in patients with chronic immune thrombocytopenia

  • Authors:
    • Lan Dai
    • Linyan He
    • Zhaoyue Wang
    • Xia Bai
    • Yang He
    • Lijuan Cao
    • Mingqing Zhu
    • Changgeng Ruan
  • View Affiliations / Copyright

    Affiliations: Jiangsu Institute of Hematology, Key Laboratory of Thrombosis and Hemostasis of Ministry of Health, The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215006, P.R. China
    Copyright: © Dai et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 2471-2477
    |
    Published online on: July 23, 2018
       https://doi.org/10.3892/etm.2018.6508
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Abstract

The present study aimed to illuminate the role of circulating T follicular helper (TFH) cells in patients diagnosed with chronic immune thrombocytopenia (cITP). Fifty‑four patients with cITP and 30 age‑matched healthy control subjects were enrolled in the present study. TFH cell frequencies, expression of CD4+ TFH cell‑associated cytokines, including interleukin (IL)‑2, IL‑4, IL‑10 and IL‑21 and associated regulatory mRNA expression levels including Bcl‑6, c‑Maf, Blimp‑1 and PD‑1 pre‑ and post‑treatment with intravenous immunoglobulin and corticosteroids, were detected by flow cytometry, ELISA and reverse transcription‑quantitative polymerase chain reaction, respectively. TFH cell frequencies of patients were significantly higher compared with healthy controls pre‑treatment (P<0.05). Following treatment, significantly decreased percentages of TFH cells were present in cITP responders (P<0.05). Correlation analysis revealed that the number of TFH cells was negatively correlated with the platelet count in the peripheral blood. Furthermore, analysis of inflammatory cytokines indicated significant differences in serum interleukin (IL)‑21 and IL‑10 between pretreated patients and healthy controls (P<0.05). Additionally, transcription factor B‑cell lymphoma (Bcl)‑6, c‑Maf and programmed death‑ligand (PD)‑1 mRNA expression levels were significantly different between cITP patients prior to treatment and the healthy controls (P<0.05). However, the expression levels of Bcl‑6, C‑Maf and PD‑1 mRNA were significantly changed post‑treatment (P<0.05). These data demonstrated that circulating TFH cells and CD4+ TFH cell‑associated cytokines may serve a role in cITP. The findings suggest that the overactivation of TFH cells may contribute to the immunopathogenesis of cITP, thus blocking the pathway of TFH cells may be reasonable for therapeutic intervention.
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Copy and paste a formatted citation
Spandidos Publications style
Dai L, He L, Wang Z, Bai X, He Y, Cao L, Zhu M and Ruan C: Altered circulating T follicular helper cells in patients with chronic immune thrombocytopenia. Exp Ther Med 16: 2471-2477, 2018.
APA
Dai, L., He, L., Wang, Z., Bai, X., He, Y., Cao, L. ... Ruan, C. (2018). Altered circulating T follicular helper cells in patients with chronic immune thrombocytopenia. Experimental and Therapeutic Medicine, 16, 2471-2477. https://doi.org/10.3892/etm.2018.6508
MLA
Dai, L., He, L., Wang, Z., Bai, X., He, Y., Cao, L., Zhu, M., Ruan, C."Altered circulating T follicular helper cells in patients with chronic immune thrombocytopenia". Experimental and Therapeutic Medicine 16.3 (2018): 2471-2477.
Chicago
Dai, L., He, L., Wang, Z., Bai, X., He, Y., Cao, L., Zhu, M., Ruan, C."Altered circulating T follicular helper cells in patients with chronic immune thrombocytopenia". Experimental and Therapeutic Medicine 16, no. 3 (2018): 2471-2477. https://doi.org/10.3892/etm.2018.6508
Copy and paste a formatted citation
x
Spandidos Publications style
Dai L, He L, Wang Z, Bai X, He Y, Cao L, Zhu M and Ruan C: Altered circulating T follicular helper cells in patients with chronic immune thrombocytopenia. Exp Ther Med 16: 2471-2477, 2018.
APA
Dai, L., He, L., Wang, Z., Bai, X., He, Y., Cao, L. ... Ruan, C. (2018). Altered circulating T follicular helper cells in patients with chronic immune thrombocytopenia. Experimental and Therapeutic Medicine, 16, 2471-2477. https://doi.org/10.3892/etm.2018.6508
MLA
Dai, L., He, L., Wang, Z., Bai, X., He, Y., Cao, L., Zhu, M., Ruan, C."Altered circulating T follicular helper cells in patients with chronic immune thrombocytopenia". Experimental and Therapeutic Medicine 16.3 (2018): 2471-2477.
Chicago
Dai, L., He, L., Wang, Z., Bai, X., He, Y., Cao, L., Zhu, M., Ruan, C."Altered circulating T follicular helper cells in patients with chronic immune thrombocytopenia". Experimental and Therapeutic Medicine 16, no. 3 (2018): 2471-2477. https://doi.org/10.3892/etm.2018.6508
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