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Article

Regulatory role of microRNA‑185 in the recovery process after ankle fracture

  • Authors:
    • Deping Sun
    • Juntao Liu
    • Qingpeng Shi
    • Haibo Mu
    • Dongsheng Zhou
  • View Affiliations / Copyright

    Affiliations: Department of Orthopedics, Shandong Provincial Hospital Affiliated to Shandong University, Jinan, Shandong 250021, P.R. China, Department of Orthopedic Trauma, Yantai Affiliated Hospital of Binzhou Medical University, Yantai, Shandong 264000, P.R. China
  • Pages: 3261-3267
    |
    Published online on: July 27, 2018
       https://doi.org/10.3892/etm.2018.6534
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Abstract

The present study aimed to investigate the expression of microRNA (miR)‑185 in the bone and blood tissues following ankle fracture, and its regulatory mechanism in the ankle fracture recovery process. In total, 28 patients with ankle fractures were included, including 15 cases receiving surgical treatment within 1‑7 days after fracture, and 13 cases receiving surgery within 8‑14 days after fracture. Reverse transcription‑quantitative polymerase chain reaction was performed to detect the mRNA expression levels. Western blot analysis and ELISA were used to determine the protein expression levels. Bioinformatics analysis and dual‑luciferase reporter assay were applied to predict and confirm the upstream regulator of tumor growth factor (TGF)‑β1. An MTT assay was performed to assess the cell proliferation. Compared with the 1‑7‑day surgery group, the mRNA and protein expression levels of TGF‑β1 were significantly elevated, while the expression levels of miR‑185 were significantly declined in the bone and blood tissues in the 8‑14‑day surgery group. Bioinformatics analysis and dual‑luciferase reporter assay predicted and confirmed that TGF‑β1 was the direct target gene of miR‑185. Moreover, upregulated expression of miR‑185 significantly decreased the protein expression levels of TGF‑β1 and reduced the proliferating activity of hFOB1.19 cells. Within two weeks after ankle fracture, the expression levels of TGF‑β1 are significantly upregulated in the bone and blood tissues, which may have been associated with the downregulated expression of miR‑185. miR‑185 may modulate TGF‑β1 to regulate the recovery of ankle fracture. These findings may contribute to the understanding of the biological functions and effects of miRNA‑185 and TGF‑β1 in ankle fractures.
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Copy and paste a formatted citation
Spandidos Publications style
Sun D, Liu J, Shi Q, Mu H and Zhou D: Regulatory role of microRNA‑185 in the recovery process after ankle fracture. Exp Ther Med 16: 3261-3267, 2018.
APA
Sun, D., Liu, J., Shi, Q., Mu, H., & Zhou, D. (2018). Regulatory role of microRNA‑185 in the recovery process after ankle fracture. Experimental and Therapeutic Medicine, 16, 3261-3267. https://doi.org/10.3892/etm.2018.6534
MLA
Sun, D., Liu, J., Shi, Q., Mu, H., Zhou, D."Regulatory role of microRNA‑185 in the recovery process after ankle fracture". Experimental and Therapeutic Medicine 16.4 (2018): 3261-3267.
Chicago
Sun, D., Liu, J., Shi, Q., Mu, H., Zhou, D."Regulatory role of microRNA‑185 in the recovery process after ankle fracture". Experimental and Therapeutic Medicine 16, no. 4 (2018): 3261-3267. https://doi.org/10.3892/etm.2018.6534
Copy and paste a formatted citation
x
Spandidos Publications style
Sun D, Liu J, Shi Q, Mu H and Zhou D: Regulatory role of microRNA‑185 in the recovery process after ankle fracture. Exp Ther Med 16: 3261-3267, 2018.
APA
Sun, D., Liu, J., Shi, Q., Mu, H., & Zhou, D. (2018). Regulatory role of microRNA‑185 in the recovery process after ankle fracture. Experimental and Therapeutic Medicine, 16, 3261-3267. https://doi.org/10.3892/etm.2018.6534
MLA
Sun, D., Liu, J., Shi, Q., Mu, H., Zhou, D."Regulatory role of microRNA‑185 in the recovery process after ankle fracture". Experimental and Therapeutic Medicine 16.4 (2018): 3261-3267.
Chicago
Sun, D., Liu, J., Shi, Q., Mu, H., Zhou, D."Regulatory role of microRNA‑185 in the recovery process after ankle fracture". Experimental and Therapeutic Medicine 16, no. 4 (2018): 3261-3267. https://doi.org/10.3892/etm.2018.6534
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