Evaluation of the diagnostic value of the immunoblotting and ELISA tests using recombinant Em18 antigen in human alveolar echinococcosis from Xingjiang China

Bi,Xiao‑Juan; Shao,Yin‑Mei; Li,Liang; Wang,Yan; Zhang,Chuan‑Shan; Lü,Guo‑Dong; Aji,Tuerganaili; Li,Jun; Zhang,Wen‑Bao; Wen,Hao; Lin,Ren‑Yong

doi:10.3892/etm.2018.6555

Evaluation of the diagnostic value of the immunoblotting and ELISA tests using recombinant Em18 antigen in human alveolar echinococcosis from Xingjiang China

Authors:
- Xiao‑Juan Bi
- Yin‑Mei Shao
- Liang Li
- Yan Wang
- Chuan‑Shan Zhang
- Guo‑Dong Lü
- Tuerganaili Aji
- Jun Li
- Wen‑Bao Zhang
- Hao Wen
- Ren‑Yong Lin
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Affiliations: State Key Laboratory of Pathogenesis, Prevention and Treatment of High Incidence Diseases in Central Asia, Clinical Medicine Institute, The First Affiliated Hospital of Xinjiang Medical University, Urumqi, Xinjiang 830054, P.R. China, Hepatobiliary & Hydatid Department, Digestive and Vascular Surgery Centre, The First Affiliated Hospital of Xinjiang Medical University, Urumqi, Xinjiang 830054, P.R. China
Published online on: August 1, 2018 https://doi.org/10.3892/etm.2018.6555
Pages: 3155-3160

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Abstract

Alveolar echinococcosis (AE) is a prevalent epidemic in the northern hemisphere, especially in central Europe and western China. Serum diagnosis is important for patients with AE, especially during the first screening. The present study purified the recombinant Em18‑GST (rEm18‑GST), and detected its diagnostic performance in human alveolar echinococcosis patients of Xinjiang, China with immunoblotting (IB) and enzyme‑linked immunosorbent assay (ELISA). Serum samples were collected from 50 patients with AE, 222 patients with cystic echinococcosis (CE), 158 patients with other unrelated infections and 106 healthy individuals. The IB results showed that serum samples of 47 patients with AE and 12 patients with CE were rEm18‑positive. However, only one sample from patients with cancer showed a cross‑reaction with rEm18 in IB. The overall sensitivity was 94%, and the total specificity was 96.58%. For the rEm18 results using ELISA, the sera of 46 patients with AE were positive, and the overall sensitivity was 92%. In conclusion, compared with imaging tools, including computed tomography, magnetic resonance imaging and positron emission tomography, rEm18 has considerable advantages for AE serodiagnosis.

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