Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Experimental and Therapeutic Medicine
Join Editorial Board Propose a Special Issue
Print ISSN: 1792-0981 Online ISSN: 1792-1015
Journal Cover
October-2018 Volume 16 Issue 4

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
October-2018 Volume 16 Issue 4

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article Open Access

Effect of abnormal GpG methylation in the second trimester of pregnancy on adverse health risk of offspring

  • Authors:
    • Yu Shi
    • Mingxing Liu
    • Xiaolin Long
    • Dunjin Chen
    • Haiyan Zheng
    • Shiling Chen
  • View Affiliations / Copyright

    Affiliations: Center for Reproductive Medicine, Department of Obstetrics and Gynecology, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong 510515, P.R. China, Key Laboratory for Major Obstetric Diseases of Guangdong Province, Key Laboratory for Reproduction and Genetics of Guangdong Higher-Education Institutes, Guangzhou Institute of Obstetrics and Gynecology, The Third Affiliated Hospital of Guangzhou Medical University, Guangzhou, Guangdong 510150, P.R. China
    Copyright: © Shi et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 2875-2880
    |
    Published online on: August 2, 2018
       https://doi.org/10.3892/etm.2018.6560
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

Effect of abnormal GpG methylation in amniotic fluid cells during the second trimester of pregnancy on adverse health risk of offspring was investigated. In total, 237 sets of amniotic fluid cells were collected from patients who received prenatal diagnosis in the Third Affiliated Hospital of Guangzhou Medical University (Guangzhou, China) from April 2010 to October 2011. Among them, 156 sets were from singleton and 81 sets were from twins. H19 gene was amplified by PCR, and the product was purified and pyrosequencing was used to detect the DNA methylation level of GapG. Follow-up records of the birth outcomes of pregnant women's offspring were collected. Positive rate of DNA amplification in 200 cases of amniotic fluid cells was 84.4% (200/237). Average age of singleton pregnancies was higher than that of twins (P<0.05), and no significant differences were found in gestational age and PCR amplification rate (P>0.05). There was no difference in the methylation level of GapG between singleton and twins (P>0.05), but the abnormal methylation rate of GapG1 in twin fetuses was significantly higher than that of singleton (20.3 vs. 3.6%, χ2=8.364, P=0.004). Offspring sex, singleton or twins, mode of delivery, time of pregnancy, and low birth weight showed no significant effect on GapG methylation level of H19 in the second trimester of pregnancy. No offspring deformities were found regardless of the increased or decreased degree of methylation (P>0.05). The number of fetuses born may cause abnormal GapG1 methylation, but no effect of GapG methylation on the adverse health risk of offspring was found.
View Figures

Figure 1

View References

1 

McCullough LE, Miller EE, Calderwood LE, Shivappa N, Steck SE, Forman MR, Mendez A M, Maguire R, Fuemmeler BF, Kollins SH, et al: Maternal inflammatory diet and adverse pregnancy outcomes: Circulating cytokines and genomic imprinting as potential regulators? Epigenetics. 12:688–697. 2017. View Article : Google Scholar : PubMed/NCBI

2 

Kusinski LC, Cooper WN, Sandovici I and Constância M: Contribution of placental genomic imprinting and identification of imprinted genesThe Guide to Investigation of Mouse Pregnancy. Croy A, Yamada AT, DeMayo FJ and Adamson SL: Academic Press; Cambridge, MA, USA: pp. 275–284. 2014, https://doi.org/10.1016/B978-0-12-394445-0.00023-0 View Article : Google Scholar

3 

Ollikainen M, Smith KR, Joo EJ, Ng HK, Andronikos R, Novakovic B, Aziz Abdul NK, Carlin JB, Morley R, Saffery R, et al: DNA methylation analysis of multiple tissues from newborn twins reveals both genetic and intrauterine components to variation in the human neonatal epigenome. Hum Mol Genet. 19:4176–4188. 2010. View Article : Google Scholar : PubMed/NCBI

4 

Wianny F, Blachère T, Godet M, Guillermas R, Cortay V, Bourillot PY, Lefèvre A, Savatier P and Dehay C: Epigenetic status of H19/IGF2 and SNRPN imprinted genes in aborted and successfully derived embryonic stem cell lines in non-human primates. Stem Cell Res (Amst). 16:557–567. 2016. View Article : Google Scholar

5 

Zhu JQ, Si YJ, Cheng LY, Xu BZ, Wang QW, Zhang X, Wang H and Liu ZP: Sodium fluoride disrupts DNA methylation of H19 and Peg3 imprinted genes during the early development of mouse embryo. Arch Toxicol. 88:241–248. 2014. View Article : Google Scholar : PubMed/NCBI

6 

Feng Y and Rudensky AY: DNA methylation secures CD4(+) and CD8(+) T cell lineage borders. Nat Immunol. 16:681–683. 2015. View Article : Google Scholar : PubMed/NCBI

7 

Sasheva P and Grossniklaus U: Differentially methylated region-representational difference analysis (DMR-RDA): A powerful method to identify DMRs in uncharacterized genomes. Methods Mol Biol. 1456:113–125. 2017. View Article : Google Scholar : PubMed/NCBI

8 

Verloes A, Spits C, Vercammen M, Geens M, LeMaoult J, Sermon K, Coucke W and Van de Velde H: The role of methylation, DNA polymorphisms and microRNAs on HLA-G expression in human embryonic stem cells. Stem Cell Res (Amst). 19:118–127. 2017. View Article : Google Scholar

9 

Ihle MA, Fassunke J, König K, Grünewald I, Schlaak M, Kreuzberg N, Tietze L, Schildhaus HU, Büttner R and Merkelbach-Bruse S: Comparison of high resolution melting analysis, pyrosequencing, next generation sequencing and immunohistochemistry to conventional Sanger sequencing for the detection of p.V600E and non-p.V600E BRAF mutations. BMC Cancer. 14:132014. View Article : Google Scholar : PubMed/NCBI

10 

White HE, Durston VJ, Harvey JF and Cross NC: Quantitative analysis of SNRPN (correction of SRNPN) gene methylation by pyrosequencing as a diagnostic test for Prader-Willi syndrome and Angelman syndrome. Clin Chem. 52:1005–1013. 2006. View Article : Google Scholar : PubMed/NCBI

11 

Irahara N, Nosho K, Baba Y, Shima K, Lindeman NI, Hazra A, Schernhammer ES, Hunter DJ, Fuchs CS and Ogino S: Precision of pyrosequencing assay to measure LINE-1 methylation in colon cancer, normal colonic mucosa, and peripheral blood cells. J Mol Diagn. 12:177–183. 2010. View Article : Google Scholar : PubMed/NCBI

12 

Ahmadian A, Ehn M and Hober S: Pyrosequencing: History, biochemistry and future. Clin Chim Acta. 363:83–94. 2006. View Article : Google Scholar : PubMed/NCBI

13 

Barbaux S, Erwich JJ, Favaron PO, Gil S, Gallot D, Golos TG, Gonzalez-Bulnes A, Guibourdenche J, Heazell AE, Jansson T, et al: IFPA meeting 2014 workshop report: Animal models to study pregnancy pathologies; new approaches to study human placental exposure to xenobiotics; biomarkers of pregnancy pathologies; placental genetics and epigenetics; the placenta and stillbirth and fetal growth restriction. Placenta. 36 Suppl 1:S5–S10. 2015. View Article : Google Scholar : PubMed/NCBI

14 

Bliek J, Alders M, Maas SM, Oostra RJ, Mackay DM, van der Lip K, Callaway JL, Brooks A, van't Padje S, Westerveld A, et al: Lessons from BWS twins: Complex maternal and paternal hypomethylation and a common source of haematopoietic stem cells. Eur J Hum Genet. 17:1625–1634. 2009. View Article : Google Scholar : PubMed/NCBI

15 

Yamazawa K, Kagami M, Fukami M, Matsubara K and Ogata T: Monozygotic female twins discordant for Silver-Russell syndrome and hypomethylation of the H19-DMR. J Hum Genet. 53:950–955. 2008. View Article : Google Scholar : PubMed/NCBI

16 

Loke YJ, Galati JC, Morley R, Joo EJ, Novakovic B, Li X, Weinrich B, Carson N, Ollikainen M, Ng HK, et al: Association of maternal and nutrient supply line factors with DNA methylation at the imprinted IGF2/H19 locus in multiple tissues of newborn twins. Epigenetics. 8:1069–1079. 2013. View Article : Google Scholar : PubMed/NCBI

17 

Williams-Wyss O, Zhang S, MacLaughlin SM, Kleemann D, Walker SK, Suter CM, Cropley JE, Morrison JL, Roberts CT and McMillen IC: Embryo number and periconceptional undernutrition in the sheep have differential effects on adrenal epigenotype, growth, and development. Am J Physiol Endocrinol Metab. 307:E141–E150. 2014. View Article : Google Scholar : PubMed/NCBI

18 

Hoyo C, Fortner K, Murtha AP, Schildkraut JM, Soubry A, Demark-Wahnefried W, Jirtle RL, Kurtzberg J, Forman MR, Overcash F, et al: Association of cord blood methylation fractions at imprinted insulin-like growth factor 2 (IGF2), plasma IGF2, and birth weight. Cancer Causes Control. 23:635–645. 2012. View Article : Google Scholar : PubMed/NCBI

19 

Petry CJ, Seear RV, Wingate DL, Acerini CL, Ong KK, Hughes IA and Dunger DB: Maternally transmitted foetal H19 variants and associations with birth weight. Hum Genet. 130:663–670. 2011. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Shi Y, Liu M, Long X, Chen D, Zheng H and Chen S: Effect of abnormal GpG methylation in the second trimester of pregnancy on adverse health risk of offspring. Exp Ther Med 16: 2875-2880, 2018.
APA
Shi, Y., Liu, M., Long, X., Chen, D., Zheng, H., & Chen, S. (2018). Effect of abnormal GpG methylation in the second trimester of pregnancy on adverse health risk of offspring. Experimental and Therapeutic Medicine, 16, 2875-2880. https://doi.org/10.3892/etm.2018.6560
MLA
Shi, Y., Liu, M., Long, X., Chen, D., Zheng, H., Chen, S."Effect of abnormal GpG methylation in the second trimester of pregnancy on adverse health risk of offspring". Experimental and Therapeutic Medicine 16.4 (2018): 2875-2880.
Chicago
Shi, Y., Liu, M., Long, X., Chen, D., Zheng, H., Chen, S."Effect of abnormal GpG methylation in the second trimester of pregnancy on adverse health risk of offspring". Experimental and Therapeutic Medicine 16, no. 4 (2018): 2875-2880. https://doi.org/10.3892/etm.2018.6560
Copy and paste a formatted citation
x
Spandidos Publications style
Shi Y, Liu M, Long X, Chen D, Zheng H and Chen S: Effect of abnormal GpG methylation in the second trimester of pregnancy on adverse health risk of offspring. Exp Ther Med 16: 2875-2880, 2018.
APA
Shi, Y., Liu, M., Long, X., Chen, D., Zheng, H., & Chen, S. (2018). Effect of abnormal GpG methylation in the second trimester of pregnancy on adverse health risk of offspring. Experimental and Therapeutic Medicine, 16, 2875-2880. https://doi.org/10.3892/etm.2018.6560
MLA
Shi, Y., Liu, M., Long, X., Chen, D., Zheng, H., Chen, S."Effect of abnormal GpG methylation in the second trimester of pregnancy on adverse health risk of offspring". Experimental and Therapeutic Medicine 16.4 (2018): 2875-2880.
Chicago
Shi, Y., Liu, M., Long, X., Chen, D., Zheng, H., Chen, S."Effect of abnormal GpG methylation in the second trimester of pregnancy on adverse health risk of offspring". Experimental and Therapeutic Medicine 16, no. 4 (2018): 2875-2880. https://doi.org/10.3892/etm.2018.6560
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team