Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Experimental and Therapeutic Medicine
Join Editorial Board Propose a Special Issue
Print ISSN: 1792-0981 Online ISSN: 1792-1015
Journal Cover
October-2018 Volume 16 Issue 4

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
October-2018 Volume 16 Issue 4

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article Open Access

Application of karyotype analysis combined with BACs-on-Beads for prenatal diagnosis

  • Authors:
    • Yuan Fang
    • Guangming Wang
    • Lize Gu
    • Jingjing Wang
    • Feng Suo
    • Maosheng Gu
    • Lingshan Gou
  • View Affiliations / Copyright

    Affiliations: Center for Genetic Medicine, Xuzhou Maternity and Child Health Care Hospital, Xuzhou, Jiangsu 221009, P.R. China, Department of Radiology, Xuzhou Central Hospital, Xuzhou, Jiangsu 221009, P.R. China
    Copyright: © Fang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 2895-2900
    |
    Published online on: August 6, 2018
       https://doi.org/10.3892/etm.2018.6574
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

This study explored the clinical application of karyotype analysis combined with BACs-on-Beads (BoBs) technology in prenatal diagnosis. A total of 558 pregnant women who were admitted to Xuzhou Maternity and Child Health Care Hospital from July 2015 to June 2017 were enrolled in this study. All the subjects underwent amniocentesis. BoBs assay was performed for subjects in the observation group, and karyotype analysis was performed for subjects in the control group. The main technical indicators of subjects in the two groups were summarized, and cases of chromosome abnormalities were further evaluated. Clinical follow-up of their pregnancy and neonatal birth was undertaken. Finally, the chromosomal manifestations of these patients were compared with those of normal male and normal female, as well as common chromosomal abnormalities. All 558 pregnant women underwent amniocentesis again. Karyotype analysis combined with BoBs assay of amniotic fluid was performed. Cases of chromosomal abnormalities detected were: 75 cases of trisomy 21, 20 cases of trisomy 18, 1 case of trisomy 13, 27 cases of sex chromosomal abnormalities, 12 cases of balanced chromosome translocation, and 2 cases of chromosome microdeletion. The results indicated that karyotype analysis combined with BoBs technology for prenatal diagnosis was easy to perform, and provided quick results with high accuracy. The two testing methods were complementary to each other, which significantly improved the diagnostic rate of chromosomal abnormalities thus reducing birth defects and guiding continued pregnancy of high-risk pregnant women.
View Figures

Figure 1

Figure 2

Figure 3

View References

1 

Bianca I, Geraci G, Gulizia MM, Assenza Egidy G, Barone C, Campisi M, Alaimo A, Adorisio R, Comoglio F, Favilli S, et al: Consensus Document of the Italian Association of Hospital Cardiologists (ANMCO), Italian Society of Pediatric Cardiology (SICP), and Italian Society of Gynaecologists and Obstetrics (SIGO): Pregnancy and congenital heart diseases. Eur Heart J Suppl. 19 Suppl D:D256–292. 2017. View Article : Google Scholar : PubMed/NCBI

2 

Pandya VK and Sutariya HC: Unilateral multicystic renal dysplasia: Prenatal diagnosis on ultrasound. Saudi J Kidney Dis Transpl. 28:916–920. 2017.PubMed/NCBI

3 

Choy KW, Kwok YK, Cheng YK, Wong KM, Wong HK, Leung KO, Suen KW, Adler K, Wang CC, Lau TK, et al: Diagnostic accuracy of the BACs-on-Beads™ assay versus karyotyping for prenatal detection of chromosomal abnormalities: A retrospective consecutive case series. BJOG. 121:1245–1252. 2014. View Article : Google Scholar : PubMed/NCBI

4 

Grati FR, Gomes Molina D, Ferreira JC, Dupont C, Alesi V, Gouas L, Horelli-Kuitunen N, Choy KW, García-Herrero S, de la Vega AG, et al: Prevalence of recurrent pathogenic microdeletions and microduplications in over 9500 pregnancies. Prenat Diagn. 35:801–809. 2015. View Article : Google Scholar : PubMed/NCBI

5 

Marcato L, Turolla L, Pompilii E, Dupont C, Gruchy N, De Toffol S, Bracalente G, Bacrot S, Troilo E, Tabet AC, et al: Prenatal phenotype of Williams-Beuren syndrome and of the reciprocal duplication syndrome. Clin Case Rep. 2:25–32. 2014. View Article : Google Scholar : PubMed/NCBI

6 

Rosenfeld JA, Morton SA, Hummel C, Sulpizio SG, McDaniel LD, Schultz RA, Torchia BS, Ravnan JB, Ellison JW and Fisher AJ: Experience using a rapid assay for aneuploidy and microdeletion/microduplication detection in over 2,900 prenatal specimens. Fetal Diagn Ther. 36:231–241. 2014. View Article : Google Scholar : PubMed/NCBI

7 

Mei J, Wang H and Zhan L: 10p15.3p13 duplication inherited from paternal balance translocation (46,XY,t(5;10)(q35.1;p13)) identified on non-invasive prenatal testing. J Obstet Gynaecol Res. 43:1076–1079. 2017. View Article : Google Scholar : PubMed/NCBI

8 

García-Herrero S, Campos-Galindo I, Martínez-Conejero JA, Serra V, Olmo I, Lara C, Simón C and Rubio C: BACs-on-Beads technology: A reliable test for rapid detection of aneuploidies and microdeletions in prenatal diagnosis. BioMed Res Int. 2014:5902982014. View Article : Google Scholar : PubMed/NCBI

9 

Choy RK, Chen Y, Sun XF, Kwok YK and Leung TY: BACs-on-beads: A new robust and rapid detection method for prenatal diagnosis. Expert Rev Mol Diagn. 14:273–280. 2014. View Article : Google Scholar : PubMed/NCBI

10 

Piotrowski K, Halec W, Wegrzynowski J, Pietrzyk A, Henkelman M and Zajaczek S: Prenatal diagnosis of Langer-Giedion Syndrome confirmed by BACs-on-Beads technique. Ginekol Pol. 85:66–69. 2014. View Article : Google Scholar : PubMed/NCBI

11 

Ragni MV: Prenatal diagnosis by droplet digital PCR. Blood. 130:240–241. 2017. View Article : Google Scholar : PubMed/NCBI

12 

Łaczmańska I and Stembalska A: New molecular methods in prenatal invasive diagnostics. Ginekol Pol. 84:871–876. 2013.(In Polish). View Article : Google Scholar : PubMed/NCBI

13 

Baxter L and Adayapalam N: A comparative study of standard cytogenetic evaluation and molecular karyotyping for products of conception. Diagn Mol Pathol. 22:228–235. 2013. View Article : Google Scholar : PubMed/NCBI

14 

Kiiski K, Roovere T, Zordania R, von Koskull H and Horelli-Kuitunen N: Prenatal diagnosis of 17p13.1p13.3 duplication. Case Rep Med. 2012:8405382012. View Article : Google Scholar : PubMed/NCBI

15 

Piotrowski K, Henkelman M and Zajaczek S: Will the new molecular karyotyping BACs-on-Beads technique replace the traditional cytogenetic prenatal diagnostics? Preliminary reports. Ginekol Pol. 83:284–290. 2012.(In Polish). PubMed/NCBI

16 

Vialard F, Simoni G, Gomes DM, Abourra A, De Toffol S, Bru F, Romero Martinez MC, Nitsch L, Bouhanna P, Marcato L, et al: Prenatal BACs-on-Beads™: The prospective experience of five prenatal diagnosis laboratories. Prenat Diagn. 32:329–335. 2012. View Article : Google Scholar : PubMed/NCBI

17 

Popowski T, Vialard F, Leroy B, Bault JP and Molina-Gomes D: Williams-Beuren syndrome: The prenatal phenotype. Am J Obstet Gynecol. 205:e6–e8. 2011. View Article : Google Scholar : PubMed/NCBI

18 

Shaffer LG, Coppinger J, Morton SA, Alliman S, Burleson J, Traylor R, Walker C, Byerly S, Lamb AN, Schultz R, et al: The development of a rapid assay for prenatal testing of common aneuploidies and microdeletion syndromes. Prenat Diagn. 31:778–787. 2011. View Article : Google Scholar : PubMed/NCBI

19 

Fox KA and Lee W: Prenatal diagnosis and evaluation of abnormal placentation. Clin Obstet Gynecol. 60:596–607. 2017. View Article : Google Scholar : PubMed/NCBI

20 

Salvador Llorens R, Sainz Viegas A, Filardi Montoya A, Fornas Montoliu G and Serrano Menor F: Evaluation of the fetal cerebellum by magnetic resonance imaging. Radiologia. 59:380–390. 2017.(In English, and Spanish). PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Fang Y, Wang G, Gu L, Wang J, Suo F, Gu M and Gou L: Application of karyotype analysis combined with BACs-on-Beads for prenatal diagnosis. Exp Ther Med 16: 2895-2900, 2018.
APA
Fang, Y., Wang, G., Gu, L., Wang, J., Suo, F., Gu, M., & Gou, L. (2018). Application of karyotype analysis combined with BACs-on-Beads for prenatal diagnosis. Experimental and Therapeutic Medicine, 16, 2895-2900. https://doi.org/10.3892/etm.2018.6574
MLA
Fang, Y., Wang, G., Gu, L., Wang, J., Suo, F., Gu, M., Gou, L."Application of karyotype analysis combined with BACs-on-Beads for prenatal diagnosis". Experimental and Therapeutic Medicine 16.4 (2018): 2895-2900.
Chicago
Fang, Y., Wang, G., Gu, L., Wang, J., Suo, F., Gu, M., Gou, L."Application of karyotype analysis combined with BACs-on-Beads for prenatal diagnosis". Experimental and Therapeutic Medicine 16, no. 4 (2018): 2895-2900. https://doi.org/10.3892/etm.2018.6574
Copy and paste a formatted citation
x
Spandidos Publications style
Fang Y, Wang G, Gu L, Wang J, Suo F, Gu M and Gou L: Application of karyotype analysis combined with BACs-on-Beads for prenatal diagnosis. Exp Ther Med 16: 2895-2900, 2018.
APA
Fang, Y., Wang, G., Gu, L., Wang, J., Suo, F., Gu, M., & Gou, L. (2018). Application of karyotype analysis combined with BACs-on-Beads for prenatal diagnosis. Experimental and Therapeutic Medicine, 16, 2895-2900. https://doi.org/10.3892/etm.2018.6574
MLA
Fang, Y., Wang, G., Gu, L., Wang, J., Suo, F., Gu, M., Gou, L."Application of karyotype analysis combined with BACs-on-Beads for prenatal diagnosis". Experimental and Therapeutic Medicine 16.4 (2018): 2895-2900.
Chicago
Fang, Y., Wang, G., Gu, L., Wang, J., Suo, F., Gu, M., Gou, L."Application of karyotype analysis combined with BACs-on-Beads for prenatal diagnosis". Experimental and Therapeutic Medicine 16, no. 4 (2018): 2895-2900. https://doi.org/10.3892/etm.2018.6574
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team