Open Access

MicroRNA‑139‑5p elevates skeletal myogenic differentiation of human adult dental pulp stem cells through Wnt/β‑catenin signaling pathway

  • Authors:
    • Yufei Xie
    • Gang Shen
  • View Affiliations

  • Published online on: August 7, 2018     https://doi.org/10.3892/etm.2018.6585
  • Pages: 2835-2842
  • Copyright: © Xie et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The aim of the present study was to identify a microRNA (miRNA or miR)‑based biomarker and therapeutic target for skeletal myogenic differentiation of human adult dental pulp stem cells (ADSCs). miRNA expression was measured using reverse transcription‑quantitative polymerase chain reaction; cell viability assay and lactate dehydrogenase (LDH) activity levels were measured using MTT and LDH activity kits, respectively. Apoptosis assay and caspase‑3/9 activity levels were measured using flow cytometry and caspase‑3/9 activity kits, respectively. Western blot analysis and immunofluorescence microscopy measured the protein expression of myocyte‑specific enhancer factor 2C, myogenic differentiation 1, myosin heavy chain, Wnt and β‑catenin. Overexpression of miR‑139‑5p promoted cell growth and induced skeletal myogenic differentiation of ADSCs. Downregulation of miR‑139‑5p inhibited cell growth and reduced skeletal myogenic differentiation of ADSCs. Overexpression of miR‑139‑5p induced Wnt/β‑catenin signaling pathway and Wnt/β‑catenin signaling pathway was suppressed by anti‑miR‑139‑5p in ADSCs. Wnt inhibitor reduced the effect of miR‑139‑5p on skeletal myogenic differentiation of ADSCs. In conclusion, miR‑139‑5p elevates skeletal myogenic differentiation of human ADSCs through the Wnt/β‑catenin signaling pathway.
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October-2018
Volume 16 Issue 4

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Xie Y and Xie Y: MicroRNA‑139‑5p elevates skeletal myogenic differentiation of human adult dental pulp stem cells through Wnt/β‑catenin signaling pathway. Exp Ther Med 16: 2835-2842, 2018
APA
Xie, Y., & Xie, Y. (2018). MicroRNA‑139‑5p elevates skeletal myogenic differentiation of human adult dental pulp stem cells through Wnt/β‑catenin signaling pathway. Experimental and Therapeutic Medicine, 16, 2835-2842. https://doi.org/10.3892/etm.2018.6585
MLA
Xie, Y., Shen, G."MicroRNA‑139‑5p elevates skeletal myogenic differentiation of human adult dental pulp stem cells through Wnt/β‑catenin signaling pathway". Experimental and Therapeutic Medicine 16.4 (2018): 2835-2842.
Chicago
Xie, Y., Shen, G."MicroRNA‑139‑5p elevates skeletal myogenic differentiation of human adult dental pulp stem cells through Wnt/β‑catenin signaling pathway". Experimental and Therapeutic Medicine 16, no. 4 (2018): 2835-2842. https://doi.org/10.3892/etm.2018.6585