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Article Open Access

MicroRNA‑139‑5p elevates skeletal myogenic differentiation of human adult dental pulp stem cells through Wnt/β‑catenin signaling pathway

  • Authors:
    • Yufei Xie
    • Gang Shen
  • View Affiliations / Copyright

    Affiliations: Department of Orthodontics, Ninth People's Hospital, Shanghai JiaoTong University School of Medicine, Shanghai Key Laboratory of Stomatology and Shanghai Research Institute of Stomatology, National Clinical Research Center of Stomatology, Shanghai 200011, P.R. China
    Copyright: © Xie et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 2835-2842
    |
    Published online on: August 7, 2018
       https://doi.org/10.3892/etm.2018.6585
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Abstract

The aim of the present study was to identify a microRNA (miRNA or miR)‑based biomarker and therapeutic target for skeletal myogenic differentiation of human adult dental pulp stem cells (ADSCs). miRNA expression was measured using reverse transcription‑quantitative polymerase chain reaction; cell viability assay and lactate dehydrogenase (LDH) activity levels were measured using MTT and LDH activity kits, respectively. Apoptosis assay and caspase‑3/9 activity levels were measured using flow cytometry and caspase‑3/9 activity kits, respectively. Western blot analysis and immunofluorescence microscopy measured the protein expression of myocyte‑specific enhancer factor 2C, myogenic differentiation 1, myosin heavy chain, Wnt and β‑catenin. Overexpression of miR‑139‑5p promoted cell growth and induced skeletal myogenic differentiation of ADSCs. Downregulation of miR‑139‑5p inhibited cell growth and reduced skeletal myogenic differentiation of ADSCs. Overexpression of miR‑139‑5p induced Wnt/β‑catenin signaling pathway and Wnt/β‑catenin signaling pathway was suppressed by anti‑miR‑139‑5p in ADSCs. Wnt inhibitor reduced the effect of miR‑139‑5p on skeletal myogenic differentiation of ADSCs. In conclusion, miR‑139‑5p elevates skeletal myogenic differentiation of human ADSCs through the Wnt/β‑catenin signaling pathway.
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1 

Kuo YR, Chien CM, Kuo MJ, Wang FS, Huang EY and Wang CJ: Endothelin-1 expression associated with lipid peroxidation and nuclear factor-κB activation in type 2 diabetes mellitus patients with angiopathy and limb amputation. Plast Reconstr Surg. 137:187e–195e. 2016. View Article : Google Scholar : PubMed/NCBI

2 

Tsang KK, Kwong EW, Woo KY, To TS, Chung JW and Wong TK: The anti-inflammatory and antibacterial action of nanocrystalline silver and manuka honey on the molecular alternation of diabetic foot ulcer: A comprehensive literature review. Evid Based Complement Alternat Med. 2015:2182832015. View Article : Google Scholar : PubMed/NCBI

3 

Zhang E, Gao B, Yang L, Wu X and Wang Z: Notoginsenoside Ft1 promotes fibroblast proliferation via PI3K/Akt/mTOR signaling pathway and benefits wound healing in genetically diabetic mice. J Pharmacol Exp Ther. 356:324–332. 2016. View Article : Google Scholar : PubMed/NCBI

4 

Arya AK, Tripathi R, Kumar S and Tripathi K: Recent advances on the association of apoptosis in chronic non healing diabetic wound. World J Diabetes. 5:756–762. 2014. View Article : Google Scholar : PubMed/NCBI

5 

Folestad A, Ålund M, Asteberg S, Fowelin J, Aurell Y, Göthlin J and Cassuto J: IL-17 cytokines in bone healing of diabetic Charcot arthropathy patients: A prospective 2 year follow-up study. J Foot Ankle Res. 8:392015. View Article : Google Scholar : PubMed/NCBI

6 

Yang RH, Lin J, Hou XH, Cao R, Yu F, Liu HQ, Ji AL, Xu XN, Zhang L and Wang F: Effect of docosahexaenoic acid on hippocampal neurons in high-glucose condition: Involvement of PI3K/AKT/nuclear factor-κB-mediated inflammatory pathways. Neuroscience. 274:218–228. 2014. View Article : Google Scholar : PubMed/NCBI

7 

Padiya R, Chowdhury D, Borkar R, Srinivas R, Pal Bhadra M and Banerjee SK: Garlic attenuates cardiac oxidative stress via activation of PI3K/AKT/Nrf2-Keap1 pathway in fructose-fed diabetic rat. PLoS One. 9:e942282014. View Article : Google Scholar : PubMed/NCBI

8 

Wang M, Liang L, Li L, Han K, Li Q, Peng Y, Peng X and Zeng K: Increased miR-424-5p expression in peripheral blood mononuclear cells from patients with pemphigus. Mol Med Rep. 15:3479–3484. 2017. View Article : Google Scholar : PubMed/NCBI

9 

Chu Q, Sun Y, Cui J and Xu T: Inducible microRNA-214 contributes to the suppression of NF-κB-mediated inflammatory response via targeting myd88 gene in fish. J Biol Chem. 292:5282–5290. 2017. View Article : Google Scholar : PubMed/NCBI

10 

Yang G, Gao J, Jia Y, Yan L, Yu J and Jiang Y: Oxysophoridine through intrathecal injection induces antinociception and increases the expression of the GABAAα1 receptor in the spinal cord of mice. Planta Med. 78:874–880. 2012. View Article : Google Scholar : PubMed/NCBI

11 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI

12 

Kandhare AD, Ghosh P and Bodhankar SL: Naringin, a flavanone glycoside, promotes angiogenesis and inhibits endothelial apoptosis through modulation of inflammatory and growth factor expression in diabetic foot ulcer in rats. Chem Biol Interact. 219:101–112. 2014. View Article : Google Scholar : PubMed/NCBI

13 

Yin X, Xu Z, Zhang Z, Li L, Pan Q, Zheng F and Li H: Association of PI3K/AKT/mTOR pathway genetic variants with type 2 diabetes mellitus in Chinese. Diabetes Res Clin Pract. 128:127–135. 2017. View Article : Google Scholar : PubMed/NCBI

14 

Mi L, Chen Y, Zheng X, Li Y, Zhang Q, Mo D and Yang G: MicroRNA-139-5p suppresses 3T3-L1 preadipocyte differentiation through notch and IRS1/PI3K/Akt insulin signaling pathways. J Cell Biochem. 116:1195–1204. 2015. View Article : Google Scholar : PubMed/NCBI

15 

Wang ZH, Zhang JL, Duan YL, Zhang QS, Li GF and Zheng DL: MicroRNA-214 participates in the neuroprotective effect of Resveratrol via inhibiting α-synuclein expression in MPTP-induced Parkinson's disease mouse. Biomed Pharmacother. 74:252–256. 2015. View Article : Google Scholar : PubMed/NCBI

16 

Li D, Liu J, Guo B, Liang C, Dang L, Lu C, He X, Cheung HY, Xu L, Lu C, et al: Osteoclast-derived exosomal miR-214-3p inhibits osteoblastic bone formation. Nat Commun. 7:108722016. View Article : Google Scholar : PubMed/NCBI

17 

Mi L, Li Y, Zhang Q, Zhao C, Peng Y, Yang G and Zheng X: MicroRNA-139-5p regulates C2C12 cell myogenesis through blocking Wnt/β-catenin signaling pathway. Biochem Cell Biol. 93:8–15. 2015. View Article : Google Scholar : PubMed/NCBI

18 

Zhu L, Yang XP, Janic B, Rhaleb NE, Harding P, Nakagawa P, Peterson EL and Carretero OA: Ac-SDKP suppresses TNF-α-induced ICAM-1 expression in endothelial cells via inhibition of IκB kinase and NF-κB activation. Am J Physiol Heart Circ Physiol. 310:H1176–H1183. 2016. View Article : Google Scholar : PubMed/NCBI

19 

Ma Y, Huang D, Yang F, Tian M, Wang Y, Shen D, Wang Q, Chen Q and Zhang L: Long noncoding RNA highly upregulated in liver cancer regulates the tumor necrosis factor-α-induced apoptosis in human vascular endothelial cells. DNA Cell Biol. 35:296–300. 2016. View Article : Google Scholar : PubMed/NCBI

20 

Wang X, Ha T, Hu Y, Lu C, Liu L, Zhang X, Kao R, Kalbfleisch J, Williams D and Li C: MicroRNA-214 protects against hypoxia/reoxygenation induced cell damage and myocardial ischemia/reperfusion injury via suppression of PTEN and Bim1 expression. Oncotarget. 7:86926–86936. 2016. View Article : Google Scholar : PubMed/NCBI

21 

Luo H, Yang R, Li C, Tong Y, Fan L, Liu X and Xu C: MicroRNA-139-5p inhibits bladder cancer proliferation and self-renewal by targeting the Bmi1 oncogene. Tumour Biol. 39:10104283177184142017. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Xie Y and Shen G: MicroRNA‑139‑5p elevates skeletal myogenic differentiation of human adult dental pulp stem cells through Wnt/β‑catenin signaling pathway. Exp Ther Med 16: 2835-2842, 2018.
APA
Xie, Y., & Shen, G. (2018). MicroRNA‑139‑5p elevates skeletal myogenic differentiation of human adult dental pulp stem cells through Wnt/β‑catenin signaling pathway. Experimental and Therapeutic Medicine, 16, 2835-2842. https://doi.org/10.3892/etm.2018.6585
MLA
Xie, Y., Shen, G."MicroRNA‑139‑5p elevates skeletal myogenic differentiation of human adult dental pulp stem cells through Wnt/β‑catenin signaling pathway". Experimental and Therapeutic Medicine 16.4 (2018): 2835-2842.
Chicago
Xie, Y., Shen, G."MicroRNA‑139‑5p elevates skeletal myogenic differentiation of human adult dental pulp stem cells through Wnt/β‑catenin signaling pathway". Experimental and Therapeutic Medicine 16, no. 4 (2018): 2835-2842. https://doi.org/10.3892/etm.2018.6585
Copy and paste a formatted citation
x
Spandidos Publications style
Xie Y and Shen G: MicroRNA‑139‑5p elevates skeletal myogenic differentiation of human adult dental pulp stem cells through Wnt/β‑catenin signaling pathway. Exp Ther Med 16: 2835-2842, 2018.
APA
Xie, Y., & Shen, G. (2018). MicroRNA‑139‑5p elevates skeletal myogenic differentiation of human adult dental pulp stem cells through Wnt/β‑catenin signaling pathway. Experimental and Therapeutic Medicine, 16, 2835-2842. https://doi.org/10.3892/etm.2018.6585
MLA
Xie, Y., Shen, G."MicroRNA‑139‑5p elevates skeletal myogenic differentiation of human adult dental pulp stem cells through Wnt/β‑catenin signaling pathway". Experimental and Therapeutic Medicine 16.4 (2018): 2835-2842.
Chicago
Xie, Y., Shen, G."MicroRNA‑139‑5p elevates skeletal myogenic differentiation of human adult dental pulp stem cells through Wnt/β‑catenin signaling pathway". Experimental and Therapeutic Medicine 16, no. 4 (2018): 2835-2842. https://doi.org/10.3892/etm.2018.6585
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