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Aerobic exercise‑stimulated Klotho upregulation extends life span by attenuating the excess production of reactive oxygen species in the brain and kidney

  • Authors:
    • Naichun Ji
    • Jing Luan
    • Fengrui Hu
    • Yirong Zhao
    • Bosen Lv
    • Wen Wang
    • Meng Xia
    • Xin Zhao
    • Kejing Lao
  • View Affiliations / Copyright

    Affiliations: Department of Physical Education and Shaanxi Key Laboratory of Brain Disorders and Institute of Basic and Translational Medicine, Xi'an Medical University, Xi'an, Shaanxi 710021, P.R. China
    Copyright: © Ji et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 3511-3517
    |
    Published online on: August 13, 2018
       https://doi.org/10.3892/etm.2018.6597
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Abstract

Aerobic exercise induces many adaptive changes in the whole body and improves metabolic characteristics. Klotho, an anti‑aging gene, is mainly expressed in the brain and kidney. The roles of Klotho in the brain and kidney during aerobic exercise remain largely unknown. The present study aimed to determine whether aerobic exercise could influence the expression of Klotho, decrease reactive oxygen species (ROS) and prolong life span. Sprague Dawley rats were exercised on a motor treadmill. Klotho mRNA and protein expression levels in rat brain and kidney tissues were examined using reverse transcription‑quantitative polymerase chain reaction and western blotting, respectively. ROS production was detected following intermittent aerobic exercise (IAE) or continuous aerobic exercise (CAE). Kaplan‑Meier curve analysis demonstrated that aerobic exercise significantly improved rat survival (P<0.001). The ROS levels in rat brain and kidney tissues were decreased in the aerobic exercise groups compared with the control group (P<0.05). In addition, Klotho mRNA and protein expression levels were increased significantly following aerobic exercise compared with controls (P<0.05). There was no significant difference between the IAE and CAE groups in any experiments (P>0.05). These results suggest that aerobic exercise‑stimulated Klotho upregulation extends the life span by attenuating the excess production of ROS in the brain and kidney. As Klotho exhibits a potential anti‑aging effect, promoting Klotho expression through aerobic exercise may be a novel approach for the prevention and treatment of aging and aging‑related diseases.
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Copy and paste a formatted citation
Spandidos Publications style
Ji N, Luan J, Hu F, Zhao Y, Lv B, Wang W, Xia M, Zhao X and Lao K: Aerobic exercise‑stimulated Klotho upregulation extends life span by attenuating the excess production of reactive oxygen species in the brain and kidney. Exp Ther Med 16: 3511-3517, 2018.
APA
Ji, N., Luan, J., Hu, F., Zhao, Y., Lv, B., Wang, W. ... Lao, K. (2018). Aerobic exercise‑stimulated Klotho upregulation extends life span by attenuating the excess production of reactive oxygen species in the brain and kidney. Experimental and Therapeutic Medicine, 16, 3511-3517. https://doi.org/10.3892/etm.2018.6597
MLA
Ji, N., Luan, J., Hu, F., Zhao, Y., Lv, B., Wang, W., Xia, M., Zhao, X., Lao, K."Aerobic exercise‑stimulated Klotho upregulation extends life span by attenuating the excess production of reactive oxygen species in the brain and kidney". Experimental and Therapeutic Medicine 16.4 (2018): 3511-3517.
Chicago
Ji, N., Luan, J., Hu, F., Zhao, Y., Lv, B., Wang, W., Xia, M., Zhao, X., Lao, K."Aerobic exercise‑stimulated Klotho upregulation extends life span by attenuating the excess production of reactive oxygen species in the brain and kidney". Experimental and Therapeutic Medicine 16, no. 4 (2018): 3511-3517. https://doi.org/10.3892/etm.2018.6597
Copy and paste a formatted citation
x
Spandidos Publications style
Ji N, Luan J, Hu F, Zhao Y, Lv B, Wang W, Xia M, Zhao X and Lao K: Aerobic exercise‑stimulated Klotho upregulation extends life span by attenuating the excess production of reactive oxygen species in the brain and kidney. Exp Ther Med 16: 3511-3517, 2018.
APA
Ji, N., Luan, J., Hu, F., Zhao, Y., Lv, B., Wang, W. ... Lao, K. (2018). Aerobic exercise‑stimulated Klotho upregulation extends life span by attenuating the excess production of reactive oxygen species in the brain and kidney. Experimental and Therapeutic Medicine, 16, 3511-3517. https://doi.org/10.3892/etm.2018.6597
MLA
Ji, N., Luan, J., Hu, F., Zhao, Y., Lv, B., Wang, W., Xia, M., Zhao, X., Lao, K."Aerobic exercise‑stimulated Klotho upregulation extends life span by attenuating the excess production of reactive oxygen species in the brain and kidney". Experimental and Therapeutic Medicine 16.4 (2018): 3511-3517.
Chicago
Ji, N., Luan, J., Hu, F., Zhao, Y., Lv, B., Wang, W., Xia, M., Zhao, X., Lao, K."Aerobic exercise‑stimulated Klotho upregulation extends life span by attenuating the excess production of reactive oxygen species in the brain and kidney". Experimental and Therapeutic Medicine 16, no. 4 (2018): 3511-3517. https://doi.org/10.3892/etm.2018.6597
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