Open Access

Eugenol protects the transplanted heart against ischemia/reperfusion injury in rats by inhibiting the inflammatory response and apoptosis

  • Authors:
    • Wei Fen
    • Longyu Jin
    • Qianyi Xie
    • Lihua Huang
    • Zhibin Jiang
    • Ying Ji
    • Chunyun Li
    • Linfei Yang
    • Dianjun Wang
  • View Affiliations

  • Published online on: August 13, 2018     https://doi.org/10.3892/etm.2018.6598
  • Pages: 3464-3470
  • Copyright: © Fen et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The aim of the present study was to investigate the protective effect of eugenol on the transplanted heart and explore its mechanisms of action. Male Sprague‑Dawley rats were randomly divided into a sham group (n=10), a eugenol group (n=10 pairs, donors and recipients) and a control group (n=10 pairs, donors and recipients). The recipients in the eugenol group received an intraperitoneal injection of eugenol (20 mg/kg/day). The sham group and the control group received equal volumes of physiological saline by intraperitoneal injection. After 15 days the recipients in the control and eugenol groups underwent abdominal heterotopic heart transplantation, while the sham group received only a coeliotomy. The orthotopic hearts in the sham group and the heterotopic hearts in the eugenol and control groups, as well as the peripheral blood samples from all three groups were taken 3 h post operation for biochemical, histopathological, molecular and apoptosis analyses. Compared with the control group, the eugenol treatment significantly reduced the myocardial malondialdehyde content, serum cardiac troponin I, creatine kinase‑MB, tumor necresis factor‑α and interleukin‑6 levels (P<0.05) and significantly alleviated myocardial injury. Western blot analysis demonstrated that the protein expression of cleaved Poly (ADP‑ribose) polymerase 1, BAX and active caspase‑3 in the eugenol group were significantly decreased, while B‑cell lymphoma 2 expression was significantly increased compared with the control group (P<0.05). The myocardial apoptosis rate of the eugenol group was significantly decreased compared with the control group (P<0.05). In conclusion eugenol treatment significantly reduced myocardial injury and demonstrated protective effects for the transplanted heart.
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October-2018
Volume 16 Issue 4

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Fen W, Jin L, Xie Q, Huang L, Jiang Z, Ji Y, Li C, Yang L and Wang D: Eugenol protects the transplanted heart against ischemia/reperfusion injury in rats by inhibiting the inflammatory response and apoptosis. Exp Ther Med 16: 3464-3470, 2018
APA
Fen, W., Jin, L., Xie, Q., Huang, L., Jiang, Z., Ji, Y. ... Wang, D. (2018). Eugenol protects the transplanted heart against ischemia/reperfusion injury in rats by inhibiting the inflammatory response and apoptosis. Experimental and Therapeutic Medicine, 16, 3464-3470. https://doi.org/10.3892/etm.2018.6598
MLA
Fen, W., Jin, L., Xie, Q., Huang, L., Jiang, Z., Ji, Y., Li, C., Yang, L., Wang, D."Eugenol protects the transplanted heart against ischemia/reperfusion injury in rats by inhibiting the inflammatory response and apoptosis". Experimental and Therapeutic Medicine 16.4 (2018): 3464-3470.
Chicago
Fen, W., Jin, L., Xie, Q., Huang, L., Jiang, Z., Ji, Y., Li, C., Yang, L., Wang, D."Eugenol protects the transplanted heart against ischemia/reperfusion injury in rats by inhibiting the inflammatory response and apoptosis". Experimental and Therapeutic Medicine 16, no. 4 (2018): 3464-3470. https://doi.org/10.3892/etm.2018.6598