Open Access

miR‑29b affects neurocyte apoptosis by targeting MCL‑1 during cerebral ischemia/reperfusion injury

  • Authors:
    • Zhi Huang
    • Lu Lu
    • Tianpeng Jiang
    • Shuai Zhang
    • Yaping Shen
    • Zhu Zheng
    • Ansu Zhao
    • Rui Gao
    • Rui Li
    • Shi Zhou
    • Jing Liu
  • View Affiliations

  • Published online on: August 20, 2018     https://doi.org/10.3892/etm.2018.6622
  • Pages: 3399-3404
  • Copyright: © Huang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The present study aimed to determine whether an miRNA (miR)‑29b inhibitor protected against cerebral ische­mia/reperfusion (I/R) injury in vitro and to investigate the underlying mechanisms. As a model for induced cerebral IR injury, N2a cells were exposed to an oxygen‑glucose deprivation/reoxygenation (OGD/R) environment. Using this model, it was demonstrated that miR‑29b was significantly upregulated compared with cells in a normal environment. The interactions between miR‑29b and myeloid cell leukemia sequence (MCL)‑1 were then investigated using dual‑luciferase assays, revealing a strong regulation of MCL‑1 through the 3'untranslated region. Using the OGD/R model, the present study additionally examined the effects of miR‑29b and miR‑29b inhibitor on cell viability and apoptosis using Cell Counting kit 8 and flow cytometry assays, respectively. miR‑29b transfection led to increased N2a cell apoptosis and reduced cell viability under an OGD/R environment. However, this effect was reversed by the miR‑29b inhibitor. Finally, the effects of miR‑29b on the expression of several Wnt‑associating proteins were examined. It was observed that B cell lymphoma‑2 was inhibited by miR‑29b, as was MCL‑1, whereas caspase‑3 expression was promoted. The miR‑29b inhibitor demonstrated the opposite effect. Overall, miR‑29b promoted neurocyte apoptosis by targeting MCL‑1 during cerebral I/R injury. The results of the present study suggest a potential novel therapeutic target for the treatment of ischemic stroke.
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October-2018
Volume 16 Issue 4

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Huang Z, Lu L, Jiang T, Zhang S, Shen Y, Zheng Z, Zhao A, Gao R, Li R, Zhou S, Zhou S, et al: miR‑29b affects neurocyte apoptosis by targeting MCL‑1 during cerebral ischemia/reperfusion injury. Exp Ther Med 16: 3399-3404, 2018
APA
Huang, Z., Lu, L., Jiang, T., Zhang, S., Shen, Y., Zheng, Z. ... Liu, J. (2018). miR‑29b affects neurocyte apoptosis by targeting MCL‑1 during cerebral ischemia/reperfusion injury. Experimental and Therapeutic Medicine, 16, 3399-3404. https://doi.org/10.3892/etm.2018.6622
MLA
Huang, Z., Lu, L., Jiang, T., Zhang, S., Shen, Y., Zheng, Z., Zhao, A., Gao, R., Li, R., Zhou, S., Liu, J."miR‑29b affects neurocyte apoptosis by targeting MCL‑1 during cerebral ischemia/reperfusion injury". Experimental and Therapeutic Medicine 16.4 (2018): 3399-3404.
Chicago
Huang, Z., Lu, L., Jiang, T., Zhang, S., Shen, Y., Zheng, Z., Zhao, A., Gao, R., Li, R., Zhou, S., Liu, J."miR‑29b affects neurocyte apoptosis by targeting MCL‑1 during cerebral ischemia/reperfusion injury". Experimental and Therapeutic Medicine 16, no. 4 (2018): 3399-3404. https://doi.org/10.3892/etm.2018.6622