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Comprehensive analysis of gene expression profiles associated with proliferative diabetic retinopathy

  • Authors:
    • Meng‑Ting Gong
    • Wen‑Xing Li
    • Qing Zhang
    • Wen‑Wen Lv
    • Zheng‑Hong He
    • Shu‑Li Zhou
    • Hui Zhang
    • Jing Wang
    • Kan He
  • View Affiliations / Copyright

    Affiliations: Center for Stem Cell and Translational Medicine, School of Life Sciences, Anhui University, Hefei, Anhui 230601, P.R. China, State Key Laboratory of Genetic Resources and Evolution, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, Yunnan 650223, P.R. China, Department of Ophthalmology, The Second Hospital of Anhui Medical University, Hefei, Anhui 230601, P.R. China, Hongqiao International Institute of Medicine, Shanghai Tongren Hospital/Faculty of Public Health, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, P.R. China, Department of Biostatistics, School of Life Sciences, Anhui University, Hefei, Anhui 230601, P.R. China
    Copyright: © Gong et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 3539-3545
    |
    Published online on: August 21, 2018
       https://doi.org/10.3892/etm.2018.6635
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Abstract

Proliferative diabetic retinopathy (PDR) is characterized by neovascularization on the surface of the retina or the optic disc, which is associated with environmental and genetic factors. However, its regulatory mechanism remains to be fully elucidated, particularly at a multiomics level. In the present study, a comprehensive analysis was performed of the gene expression profile of fibrovascular membranes (FVMs) associated with PDR, including an analysis of differentially expressed genes, functional enrichment, and regulation of transcription factors (TFs). As a result, novel marker genes of PDR were identified, including flavin containing monooxygenase 2. Furthermore, several common or specific genes, pathways and TFs have been recovered for active and inactive FVMs. In the present study, lymphoid enhancer binding factor 1 (LEF1) was identified as an upregulator in active and inactive FVMs, which is capable of activating or repressing target genes, including claudin 2, secreted phosphoprotein 1 (SPP1), and aristaless‑like homeobox 4. It was demonstrated that the Wnt/β‑catenin effector LEF1 regulating SPP1 is potentially important in PDR. The results of the present study may provide novel insights into the molecular mechanisms underlying the pathophysiology of PDR.
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1 

Graham PS, Kaidonis G, Abhary S, Gillies MC, Daniell M, Essex RW, Chang JH, Lake SR, Pal B, Jenkins AJ, et al: Genome-wide association studies for diabetic macular edema and proliferative diabetic retinopathy. BMC Med Genet. 19:712018. View Article : Google Scholar : PubMed/NCBI

2 

Huang YC, Liao WL, Lin JM, Chen CC, Liu SP, Chen SY, Lin YN, Lei YJ, Liu HT, Chen YJ and Tsai FJ: High levels of circulating endothelial progenitor cells in patients with diabetic retinopathy are positively associated with ARHGAP22 expression. Oncotarget. 9:17858–17866. 2018. View Article : Google Scholar : PubMed/NCBI

3 

Ishikawa K, Yoshida S, Kobayashi Y, Zhou Y, Nakama T, Nakao S, Sassa Y, Oshima Y, Niiro H, Akashi K, et al: Microarray analysis of gene expression in fibrovascular membranes excised from patients with proliferative diabetic retinopathy. Invest Ophthalmol Vis Sci. 56:932–946. 2015. View Article : Google Scholar : PubMed/NCBI

4 

Nathan DM: Long-term complications of diabetes mellitus. N Engl J Med. 328:1676–1685. 1993. View Article : Google Scholar : PubMed/NCBI

5 

Uhlmann K, Kovacs P, Boettcher Y, Hammes HP and Paschke R: Genetics of diabetic retinopathy. Exp Clin Endocrinol Diabetes. 114:275–294. 2006. View Article : Google Scholar : PubMed/NCBI

6 

Khalfaoui T, Lizard G, Beltaief O, Colin D, Ben Hamida J, Errais K, Ammous I, Zbiba W, Tounsi L, Zhioua R, et al: Immunohistochemical analysis of cellular adhesion molecules (ICAM-1, VCAM-1) and VEGF in fibrovascular membranes of patients with proliferative diabetic retinopathy: Preliminary study. Pathol Biol (Paris). 57:513–517. 2009. View Article : Google Scholar : PubMed/NCBI

7 

Beranek M, Kolar P, Tschoplova S, Kankova K and Vasku A: Genetic variation and plasma level of the basic fibroblast growth factor in proliferative diabetic retinopathy. Diabetes Res Clin Pract. 79:362–367. 2008. View Article : Google Scholar : PubMed/NCBI

8 

Uthra S, Raman R, Mukesh BN, Rajkumar SA, Kumari RP, Agarwal S, Paul PG, Lakshmipathy P, Gnanamoorthy P, Sharma T, et al: Diabetic retinopathy and IGF-1 gene polymorphic cytosine-adenine repeats in a Southern Indian cohort. Ophthalmic Res. 39:294–299. 2007. View Article : Google Scholar : PubMed/NCBI

9 

Cilenšek I, Mankoč S, Globočnik Petrovič M and Petrovič D: The 4a/4a genotype of the VNTR polymorphism for endothelial nitric oxide synthase (eNOS) gene predicts risk for proliferative diabetic retinopathy in Slovenian patients (Caucasians) with type 2 diabetes mellitus. Mol Biol Rep. 39:7061–7067. 2012. View Article : Google Scholar : PubMed/NCBI

10 

Crispim D, Fagundes NJ, dos Santos KG, Rheinheimer J, Bouças AP, de Souza BM, Macedo GS, Leiria LB, Gross JL and Canani LH: Polymorphisms of the UCP2 gene are associated with proliferative diabetic retinopathy in patients with diabetes mellitus. Clin Endocrinol (Oxf). 72:612–619. 2010. View Article : Google Scholar : PubMed/NCBI

11 

Santos KG, Crispim D, Canani LH, Ferrugem PT, Gross JL and Roisenberg I: Relationship of endothelial nitric oxide synthase (eNOS) gene polymorphisms with diabetic retinopathy in Caucasians with type 2 diabetes. Ophthalmic Genet. 33:23–27. 2012. View Article : Google Scholar : PubMed/NCBI

12 

Choi D, Appukuttan B, Binek SJ, Planck SR, Stout JT, Rosenbaum JT and Smith JR: Prediction of Cis-regulatory elements controlling genes differentially expressed by retinal and choroidal vascular endothelial cells. J Ocul Biol Dis Infor. 1:37–45. 2008. View Article : Google Scholar : PubMed/NCBI

13 

Gu Y, Ke G, Wang L, Zhou E, Zhu K and Wei Y: Altered expression profile of circular RNAs in the serum of patients with diabetic retinopathy revealed by microarray. Ophthalmic Res. 58:176–184. 2017. View Article : Google Scholar : PubMed/NCBI

14 

Edgar R, Domrachev M and Lash AE: Gene expression omnibus: NCBI gene expression and hybridization array data repository. Nucleic Acids Res. 30:207–210. 2002. View Article : Google Scholar : PubMed/NCBI

15 

Carvalho BS and Irizarry RA: A framework for oligonucleotide microarray preprocessing. Bioinformatics. 26:2363–2367. 2010. View Article : Google Scholar : PubMed/NCBI

16 

Huang da W, Sherman BT and Lempicki RA: Systematic and integrative analysis of large gene lists using DAVID bioinformatics resources. Nat Protoc. 4:44–57. 2009. View Article : Google Scholar : PubMed/NCBI

17 

Matys V, Kel-Margoulis OV, Fricke E, Liebich I, Land S, Barre-Dirrie A, Reuter I, Chekmenev D, Krull M, Hornischer K, et al: TRANSFAC and its module TRANSCompel: Transcriptional gene regulation in eukaryotes. Nucleic Acids Res. 34(Database Issue): D108–D110. 2006. View Article : Google Scholar : PubMed/NCBI

18 

Wingender E, Dietze P, Karas H and Knüppel R: TRANSFAC: A database on transcription factors and their DNA binding sites. Nucleic Acids Res. 24:238–241. 1996. View Article : Google Scholar : PubMed/NCBI

19 

Karoly ED and Rose RL: Sequencing, expression, and characterization of cDNA expressed flavin-containing monooxygenase 2 from mouse. J Biochem Mol Toxicol. 15:300–308. 2001. View Article : Google Scholar : PubMed/NCBI

20 

Scharf ME, Scharf DW, Bennett GW and Pittendrigh BR: Catalytic activity and expression of two flavin-containing monooxygenases from Drosophila melanogaster. Arch Insect Biochem Physiol. 57:28–39. 2004. View Article : Google Scholar : PubMed/NCBI

21 

Bennett CF, Kwon JJ, Chen C, Russell J, Acosta K, Burnaevskiy N, Crane MM, Bitto A, Vander Wende H, Simko M, et al: Transaldolase inhibition impairs mitochondrial respiration and induces a starvation-like longevity response in Caenorhabditis elegans. PLoS Genet. 13:e10066952017. View Article : Google Scholar : PubMed/NCBI

22 

Leiser SF, Miller H, Rossner R, Fletcher M, Leonard A, Primitivo M, Rintala N, Ramos FJ, Miller DL and Kaeberlein M: Cell nonautonomous activation of flavin-containing monooxygenase promotes longevity and health span. Science. 350:1375–1378. 2015. View Article : Google Scholar : PubMed/NCBI

23 

Carr T, Krishnamoorthy V, Yu S, Xue HH, Kee BL and Verykokakis M: The transcription factor lymphoid enhancer factor 1 controls invariant natural killer T cell expansion and Th2-type effector differentiation. J Exp Med. 212:793–807. 2015. View Article : Google Scholar : PubMed/NCBI

24 

Wang X, Liu Y, Chen H, Mei L, He C, Jiang L, Niu Z, Sun J, Luo H, Li J and Feng Y: LEF-1 regulates tyrosinase gene transcription in vitro. PLoS One. 10:e01431422015. View Article : Google Scholar : PubMed/NCBI

25 

Lin AY, Chua MS, Choi YL, Yeh W, Kim YH, Azzi R, Adams GA, Sainani K, van de Rijn M, So SK and Pollack JR: Comparative profiling of primary colorectal carcinomas and liver metastases identifies LEF1 as a prognostic biomarker. PLoS One. 6:e166362011. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Gong MT, Li WX, Zhang Q, Lv WW, He ZH, Zhou SL, Zhang H, Wang J and He K: Comprehensive analysis of gene expression profiles associated with proliferative diabetic retinopathy. Exp Ther Med 16: 3539-3545, 2018.
APA
Gong, M., Li, W., Zhang, Q., Lv, W., He, Z., Zhou, S. ... He, K. (2018). Comprehensive analysis of gene expression profiles associated with proliferative diabetic retinopathy. Experimental and Therapeutic Medicine, 16, 3539-3545. https://doi.org/10.3892/etm.2018.6635
MLA
Gong, M., Li, W., Zhang, Q., Lv, W., He, Z., Zhou, S., Zhang, H., Wang, J., He, K."Comprehensive analysis of gene expression profiles associated with proliferative diabetic retinopathy". Experimental and Therapeutic Medicine 16.4 (2018): 3539-3545.
Chicago
Gong, M., Li, W., Zhang, Q., Lv, W., He, Z., Zhou, S., Zhang, H., Wang, J., He, K."Comprehensive analysis of gene expression profiles associated with proliferative diabetic retinopathy". Experimental and Therapeutic Medicine 16, no. 4 (2018): 3539-3545. https://doi.org/10.3892/etm.2018.6635
Copy and paste a formatted citation
x
Spandidos Publications style
Gong MT, Li WX, Zhang Q, Lv WW, He ZH, Zhou SL, Zhang H, Wang J and He K: Comprehensive analysis of gene expression profiles associated with proliferative diabetic retinopathy. Exp Ther Med 16: 3539-3545, 2018.
APA
Gong, M., Li, W., Zhang, Q., Lv, W., He, Z., Zhou, S. ... He, K. (2018). Comprehensive analysis of gene expression profiles associated with proliferative diabetic retinopathy. Experimental and Therapeutic Medicine, 16, 3539-3545. https://doi.org/10.3892/etm.2018.6635
MLA
Gong, M., Li, W., Zhang, Q., Lv, W., He, Z., Zhou, S., Zhang, H., Wang, J., He, K."Comprehensive analysis of gene expression profiles associated with proliferative diabetic retinopathy". Experimental and Therapeutic Medicine 16.4 (2018): 3539-3545.
Chicago
Gong, M., Li, W., Zhang, Q., Lv, W., He, Z., Zhou, S., Zhang, H., Wang, J., He, K."Comprehensive analysis of gene expression profiles associated with proliferative diabetic retinopathy". Experimental and Therapeutic Medicine 16, no. 4 (2018): 3539-3545. https://doi.org/10.3892/etm.2018.6635
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