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Article Open Access

Downregulated caveolin‑1 expression serves a potential role in coronary artery spasm by inducing nitric oxide production in vitro

Corrigendum in: /10.3892/etm.2024.12535
  • Authors:
    • Xingmei Cao
    • Zhishuai Ye
    • Mingyu Jin
    • Shuai Yan
    • Xiantao Song
    • Rongchong Huang
  • View Affiliations / Copyright

    Affiliations: Department of Cardiology, Beijing An Zhen Hospital, Capital Medical University, Beijing 100029, P.R. China, Department of Cardiology, The First Affiliated Hospital of Dalian Medical University, Dalian, Liaoning 116011, P.R. China
    Copyright: © Cao et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 3567-3573
    |
    Published online on: August 22, 2018
       https://doi.org/10.3892/etm.2018.6646
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Abstract

The present study aimed to investigate the effects of downregulated caveolin‑1 (Cav‑1) expression on nitric oxide (NO) production in lipopolysaccharide (LPS)‑damaged primary human umbilical vein endothelial cells (HUVECs) in a model of coronary artery spasm (CAS) microenvironment induced by acetylcholine (ACh) treatment. Small interfering RNA (siRNA)‑mediated Cav‑1 downregulation in HUVECs was confirmed by western blotting. The cell viability and superoxide dismutase (SOD) inhibition in HUVECs incubated with LPS (0, 10, 25, 50, 75 and 100 µg/ml) were measured by cell counting kit‑8 assay and a SOD kit, respectively. Intracellular Ca2+ [(Ca2+)i] in Fluo4‑acetoxymethyl ester‑loaded cells was detected by fluorescence microscopy. NO levels in the cell culture supernatants were measured by the nitrate reductase method. The results indicated that transfection with Cav‑1 siRNA, in particular siCav‑1 (2), downregulated the Cav‑1 protein expression. LPS at a dose of 75 µg/ml induced a significant decrease in HUVECs/si‑NC and HUVECs/siCav‑1 viability compared with the other concentrations of LPS. Compared with the effects of untreated cells, SOD inhibition in HUVECs/si‑NC and HUVECs/siCav‑1 was significantly decreased by LPS (75 µg/ml). In addition, ACh stimulation caused a greater increase in [Ca2+]i in HUVECs/si‑NC as compared with LPS‑treated HUVECs/si‑NC. ACh stimulation also induced significantly higher NO levels in LPS‑treated HUVECs/siCav‑1 compared with LPS‑treated HUVECs/si‑NC cells (P<0.05). In conclusion, the downregulated Cav‑1 expression served a key role in NO production in the in vitro model of CAS induced by ACh stimulation of LPS‑damaged HUVECs.
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Copy and paste a formatted citation
Spandidos Publications style
Cao X, Ye Z, Jin M, Yan S, Song X and Huang R: Downregulated caveolin‑1 expression serves a potential role in coronary artery spasm by inducing nitric oxide production in vitro Corrigendum in /10.3892/etm.2024.12535. Exp Ther Med 16: 3567-3573, 2018.
APA
Cao, X., Ye, Z., Jin, M., Yan, S., Song, X., & Huang, R. (2018). Downregulated caveolin‑1 expression serves a potential role in coronary artery spasm by inducing nitric oxide production in vitro Corrigendum in /10.3892/etm.2024.12535. Experimental and Therapeutic Medicine, 16, 3567-3573. https://doi.org/10.3892/etm.2018.6646
MLA
Cao, X., Ye, Z., Jin, M., Yan, S., Song, X., Huang, R."Downregulated caveolin‑1 expression serves a potential role in coronary artery spasm by inducing nitric oxide production in vitro Corrigendum in /10.3892/etm.2024.12535". Experimental and Therapeutic Medicine 16.4 (2018): 3567-3573.
Chicago
Cao, X., Ye, Z., Jin, M., Yan, S., Song, X., Huang, R."Downregulated caveolin‑1 expression serves a potential role in coronary artery spasm by inducing nitric oxide production in vitro Corrigendum in /10.3892/etm.2024.12535". Experimental and Therapeutic Medicine 16, no. 4 (2018): 3567-3573. https://doi.org/10.3892/etm.2018.6646
Copy and paste a formatted citation
x
Spandidos Publications style
Cao X, Ye Z, Jin M, Yan S, Song X and Huang R: Downregulated caveolin‑1 expression serves a potential role in coronary artery spasm by inducing nitric oxide production in vitro Corrigendum in /10.3892/etm.2024.12535. Exp Ther Med 16: 3567-3573, 2018.
APA
Cao, X., Ye, Z., Jin, M., Yan, S., Song, X., & Huang, R. (2018). Downregulated caveolin‑1 expression serves a potential role in coronary artery spasm by inducing nitric oxide production in vitro Corrigendum in /10.3892/etm.2024.12535. Experimental and Therapeutic Medicine, 16, 3567-3573. https://doi.org/10.3892/etm.2018.6646
MLA
Cao, X., Ye, Z., Jin, M., Yan, S., Song, X., Huang, R."Downregulated caveolin‑1 expression serves a potential role in coronary artery spasm by inducing nitric oxide production in vitro Corrigendum in /10.3892/etm.2024.12535". Experimental and Therapeutic Medicine 16.4 (2018): 3567-3573.
Chicago
Cao, X., Ye, Z., Jin, M., Yan, S., Song, X., Huang, R."Downregulated caveolin‑1 expression serves a potential role in coronary artery spasm by inducing nitric oxide production in vitro Corrigendum in /10.3892/etm.2024.12535". Experimental and Therapeutic Medicine 16, no. 4 (2018): 3567-3573. https://doi.org/10.3892/etm.2018.6646
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