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lncRNA Malat1 modulates the maturation process, cytokine secretion and apoptosis in airway epithelial cell‑conditioned dendritic cells

  • Authors:
    • Zhoubin Li
    • Qing Zhang
    • Yutao Wu
    • Feng Hu
    • Linling Gu
    • Ting Chen
    • Weilin Wang
  • View Affiliations / Copyright

    Affiliations: Department of Lung Transplantation, First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, Zhejiang 310003, P.R. China, Department of Cardiology, First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, Zhejiang 310003, P.R. China, Key Laboratory of Precision Diagnosis and Treatment for Hepatobiliary and Pancreatic Tumor of Zhejiang Province, Division of Hepatobiliary and Pancreatic Surgery, First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, Zhejiang 310003, P.R. China
    Copyright: © Li et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 3951-3958
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    Published online on: September 4, 2018
       https://doi.org/10.3892/etm.2018.6687
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Abstract

Airway epithelial cells (AECs) are the first point of contact with airborne antigens and are able to instruct resident immune cells to appropriate immune responses. Previous studies have shown that the abnormal expression of metastasis‑associated lung adenocarcinoma transcript 1 (Malat1) was associated with tumorigenesis, progression, metastasis, and apoptosis in many cancer types. However, little is known about its functional involvement in the cross‑talk of AECs with dendritic cells (DCs). The aim of the present study was to identify Malat1 as a novel epithelial cell‑derived immune‑modulating factor that contributes to the specific inflammatory‑immune airway microenvironment. By using an in vitro co‑culture model, where layers of AECs can interact with DCs, and transfecting Malat1 siRNA in AECs, AEC‑conditioned DCs were harvested for further analysis of the celluar phenotype, secretion of inflammatory chemokines, and expression of apoptotic markers. The present study clearly demonstrated that Malat1 modulates the maturation process, pro‑inflammatory cytokine secretion and apoptosis in AECs‑conditioned DCs.
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Copy and paste a formatted citation
Spandidos Publications style
Li Z, Zhang Q, Wu Y, Hu F, Gu L, Chen T and Wang W: lncRNA Malat1 modulates the maturation process, cytokine secretion and apoptosis in airway epithelial cell‑conditioned dendritic cells. Exp Ther Med 16: 3951-3958, 2018.
APA
Li, Z., Zhang, Q., Wu, Y., Hu, F., Gu, L., Chen, T., & Wang, W. (2018). lncRNA Malat1 modulates the maturation process, cytokine secretion and apoptosis in airway epithelial cell‑conditioned dendritic cells. Experimental and Therapeutic Medicine, 16, 3951-3958. https://doi.org/10.3892/etm.2018.6687
MLA
Li, Z., Zhang, Q., Wu, Y., Hu, F., Gu, L., Chen, T., Wang, W."lncRNA Malat1 modulates the maturation process, cytokine secretion and apoptosis in airway epithelial cell‑conditioned dendritic cells". Experimental and Therapeutic Medicine 16.5 (2018): 3951-3958.
Chicago
Li, Z., Zhang, Q., Wu, Y., Hu, F., Gu, L., Chen, T., Wang, W."lncRNA Malat1 modulates the maturation process, cytokine secretion and apoptosis in airway epithelial cell‑conditioned dendritic cells". Experimental and Therapeutic Medicine 16, no. 5 (2018): 3951-3958. https://doi.org/10.3892/etm.2018.6687
Copy and paste a formatted citation
x
Spandidos Publications style
Li Z, Zhang Q, Wu Y, Hu F, Gu L, Chen T and Wang W: lncRNA Malat1 modulates the maturation process, cytokine secretion and apoptosis in airway epithelial cell‑conditioned dendritic cells. Exp Ther Med 16: 3951-3958, 2018.
APA
Li, Z., Zhang, Q., Wu, Y., Hu, F., Gu, L., Chen, T., & Wang, W. (2018). lncRNA Malat1 modulates the maturation process, cytokine secretion and apoptosis in airway epithelial cell‑conditioned dendritic cells. Experimental and Therapeutic Medicine, 16, 3951-3958. https://doi.org/10.3892/etm.2018.6687
MLA
Li, Z., Zhang, Q., Wu, Y., Hu, F., Gu, L., Chen, T., Wang, W."lncRNA Malat1 modulates the maturation process, cytokine secretion and apoptosis in airway epithelial cell‑conditioned dendritic cells". Experimental and Therapeutic Medicine 16.5 (2018): 3951-3958.
Chicago
Li, Z., Zhang, Q., Wu, Y., Hu, F., Gu, L., Chen, T., Wang, W."lncRNA Malat1 modulates the maturation process, cytokine secretion and apoptosis in airway epithelial cell‑conditioned dendritic cells". Experimental and Therapeutic Medicine 16, no. 5 (2018): 3951-3958. https://doi.org/10.3892/etm.2018.6687
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