Open Access

Anti‑fibrosis activity of combination therapy with epigallocatechin gallate, taurine and genistein by regulating glycolysis, gluconeogenesis, and ribosomal and lysosomal signaling pathways in HSC‑T6 cells

  • Authors:
    • Yan Li
    • Min Zhu
    • Yani Huo
    • Xuerong Zhang
    • Ming Liao
  • View Affiliations

  • Published online on: September 17, 2018     https://doi.org/10.3892/etm.2018.6743
  • Pages: 4329-4338
  • Copyright: © Li et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

A previous study by our group indicated that combined treatment with taurine, epigallocatechin gallate (EGCG) and genistein protects against liver fibrosis. The aim of the present study was to elucidate the antifibrotic mechanism of this combination treatment using isobaric tag for relative and absolute quantification (iTRAQ)‑based proteomics in an activated rat hepatic stellate cell (HSC) line. In the present study, HSC‑T6 cells were incubated with taurine, EGCG and genistein, and cellular proteins were extracted and processed for iTRAQ labeling. Quantification and identification of proteins was performed using two‑dimensional liquid chromatography coupled with tandem mass spectrometry. Proteomic analysis indicated that the expression of 166 proteins were significantly altered in response to combination treatment with taurine, EGCG and genistein. A total 76 of these proteins were upregulated and 90 were downregulated. Differentially expressed proteins were grouped according to their association with specific Kyoto Encyclopedia of Genes and Genomes pathways. The results indicated that the differentially expressed proteins hexokinase‑2 and lysosome‑associated membrane glycoprotein 1 were associated with glycolysis, gluconeogenesis and lysosome signaling pathways. The expression of these proteins was validated using western blot analysis; the expression of hexokinase‑2 was significantly decreased and the expression of lysosome‑associated membrane glycoprotein 1 was significantly increased in HSC‑T6 cells treated with taurine, EGCG and genistein compared with the control, respectively (P<0.05). These results were in accordance with the changes in protein expression identified using the iTRAQ approach. Therefore, the antifibrotic effect of combined therapy with taurine, EGCG and genistein may be associated with the activation of several pathways in HSCs, including glycolysis, gluconeogenesis, and the ribosome and lysosome signaling pathways. The differentially expressed proteins identified in the current study may be useful for treatment of liver fibrosis in the future.
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December-2018
Volume 16 Issue 6

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Li Y, Zhu M, Huo Y, Zhang X and Liao M: Anti‑fibrosis activity of combination therapy with epigallocatechin gallate, taurine and genistein by regulating glycolysis, gluconeogenesis, and ribosomal and lysosomal signaling pathways in HSC‑T6 cells. Exp Ther Med 16: 4329-4338, 2018
APA
Li, Y., Zhu, M., Huo, Y., Zhang, X., & Liao, M. (2018). Anti‑fibrosis activity of combination therapy with epigallocatechin gallate, taurine and genistein by regulating glycolysis, gluconeogenesis, and ribosomal and lysosomal signaling pathways in HSC‑T6 cells. Experimental and Therapeutic Medicine, 16, 4329-4338. https://doi.org/10.3892/etm.2018.6743
MLA
Li, Y., Zhu, M., Huo, Y., Zhang, X., Liao, M."Anti‑fibrosis activity of combination therapy with epigallocatechin gallate, taurine and genistein by regulating glycolysis, gluconeogenesis, and ribosomal and lysosomal signaling pathways in HSC‑T6 cells". Experimental and Therapeutic Medicine 16.6 (2018): 4329-4338.
Chicago
Li, Y., Zhu, M., Huo, Y., Zhang, X., Liao, M."Anti‑fibrosis activity of combination therapy with epigallocatechin gallate, taurine and genistein by regulating glycolysis, gluconeogenesis, and ribosomal and lysosomal signaling pathways in HSC‑T6 cells". Experimental and Therapeutic Medicine 16, no. 6 (2018): 4329-4338. https://doi.org/10.3892/etm.2018.6743