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Article Open Access

sTLR4/sMD‑2 complex alleviates LPS‑induced acute lung injury by inhibiting pro‑inflammatory cytokines and chemokine CXCL1 expression

  • Authors:
    • Jie Meng
    • Yan Zou
    • Jifei Chen
    • Fengxian Qin
    • Xiang Chen
    • Xiaoli Chen
    • Shengming Dai
  • View Affiliations / Copyright

    Affiliations: Medical Science Laboratory, The Fourth Affiliated Hospital of Guangxi Medical University, Liuzhou, Guangxi 545005, P.R. China
    Copyright: © Meng et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 4632-4638
    |
    Published online on: September 17, 2018
       https://doi.org/10.3892/etm.2018.6746
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Abstract

Activation of Toll‑like receptor 4 (TLR4) and its accessory proteins myeloid differentiation protein 2 (MD‑2) can trigger immune and inflammatory activities, and contribute to developing chronic inflammatory diseases. The formation of the TLR4/MD‑2 complex after binding to lipopolysaccharide (LPS) leads to the activation of downstream signaling pathway. The present study was designed to reveal the effect of the soluble form of the extracellular TLR4 domain and MD‑2 (sTLR4/sMD‑2) complex lacking the intracellular and transmembrane domains on various aspects of LPS‑induced inflammation in vivo and in vitro. It was demonstrated that the sTLR4/sMD‑2 complex inhibited the LPS‑induced production of tumor necrosis factor‑α, interleukin‑8 and C‑X‑C motif chemokine ligand 1 (CXCL1) in THP‑1 cells. In addition, it was revealed that the sTLR4/sMD‑2 complex significantly reduced LPS‑induced acute lung injury (ALI) with a reduction of total cells and neutrophil count, pro‑inflammatory cytokines and chemokine CXCL1 in bronchoalveolar lavage fluid. Moreover, the sTLR4/sMD‑2 complex inhibited the number of inflammatory cells in the lung of treated animals. These novel mechanisms emphasized the important role of sTLR4/sMD‑2 complex in ALI and suggested sTLR4/sMD‑2 complex could provide an anti‑inflammatory strategy for treating inflammatory diseases.
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Copy and paste a formatted citation
Spandidos Publications style
Meng J, Zou Y, Chen J, Qin F, Chen X, Chen X and Dai S: sTLR4/sMD‑2 complex alleviates LPS‑induced acute lung injury by inhibiting pro‑inflammatory cytokines and chemokine CXCL1 expression. Exp Ther Med 16: 4632-4638, 2018.
APA
Meng, J., Zou, Y., Chen, J., Qin, F., Chen, X., Chen, X., & Dai, S. (2018). sTLR4/sMD‑2 complex alleviates LPS‑induced acute lung injury by inhibiting pro‑inflammatory cytokines and chemokine CXCL1 expression. Experimental and Therapeutic Medicine, 16, 4632-4638. https://doi.org/10.3892/etm.2018.6746
MLA
Meng, J., Zou, Y., Chen, J., Qin, F., Chen, X., Chen, X., Dai, S."sTLR4/sMD‑2 complex alleviates LPS‑induced acute lung injury by inhibiting pro‑inflammatory cytokines and chemokine CXCL1 expression". Experimental and Therapeutic Medicine 16.6 (2018): 4632-4638.
Chicago
Meng, J., Zou, Y., Chen, J., Qin, F., Chen, X., Chen, X., Dai, S."sTLR4/sMD‑2 complex alleviates LPS‑induced acute lung injury by inhibiting pro‑inflammatory cytokines and chemokine CXCL1 expression". Experimental and Therapeutic Medicine 16, no. 6 (2018): 4632-4638. https://doi.org/10.3892/etm.2018.6746
Copy and paste a formatted citation
x
Spandidos Publications style
Meng J, Zou Y, Chen J, Qin F, Chen X, Chen X and Dai S: sTLR4/sMD‑2 complex alleviates LPS‑induced acute lung injury by inhibiting pro‑inflammatory cytokines and chemokine CXCL1 expression. Exp Ther Med 16: 4632-4638, 2018.
APA
Meng, J., Zou, Y., Chen, J., Qin, F., Chen, X., Chen, X., & Dai, S. (2018). sTLR4/sMD‑2 complex alleviates LPS‑induced acute lung injury by inhibiting pro‑inflammatory cytokines and chemokine CXCL1 expression. Experimental and Therapeutic Medicine, 16, 4632-4638. https://doi.org/10.3892/etm.2018.6746
MLA
Meng, J., Zou, Y., Chen, J., Qin, F., Chen, X., Chen, X., Dai, S."sTLR4/sMD‑2 complex alleviates LPS‑induced acute lung injury by inhibiting pro‑inflammatory cytokines and chemokine CXCL1 expression". Experimental and Therapeutic Medicine 16.6 (2018): 4632-4638.
Chicago
Meng, J., Zou, Y., Chen, J., Qin, F., Chen, X., Chen, X., Dai, S."sTLR4/sMD‑2 complex alleviates LPS‑induced acute lung injury by inhibiting pro‑inflammatory cytokines and chemokine CXCL1 expression". Experimental and Therapeutic Medicine 16, no. 6 (2018): 4632-4638. https://doi.org/10.3892/etm.2018.6746
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