Long non‑coding RNA HOTAIR overexpression improves premature ovarian failure by upregulating Notch‑1 expression

  • Authors:
    • Wei Zhao
    • Liwei Dong
  • View Affiliations

  • Published online on: September 17, 2018     https://doi.org/10.3892/etm.2018.6750
  • Pages: 4791-4795
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Abstract

The aim of the present study was to investigate the role of long non‑coding (lnc)RNA HOTAIR in premature ovarian failure. A total of 69 women with spontaneous premature ovarian failure and 48 healthy women were enrolled in the present study. Ovarian tissues and blood samples were harvested from each participant. The expression of HOTAIR in ovarian tissues and sera was detected using reverse transcription‑quantitative polymerase chain reaction. A receiver operator characteristic curve analysis was performed to assess the diagnostic value of HOTAIR expression in ovarian tissues and sera for premature ovarian failure. Hamster ovary cell lines overexpressing HOTAIR were established and cell apoptosis were assessed using an MTT assay. The effect of HOTAIR upregulation on the expression of Notch‑1 was measured using western blotting. The expression of HOTAIR in ovarian tissues and serum samples was significantly lower in patients with premature ovarian failure compared with healthy controls. These results suggest that ovarian and serum HOTAIR expression levels may be used to accurately predict the risk of premature ovarian failure. It was also demonstrated that HOTAIR overexpression upregulates Notch‑1 protein expression in hamster ovary cells and reduced apoptosis, whereas the Notch inhibitor L685458 ameliorated these effects. In conclusion, the results of the present study suggest that LncRNA HOTAIR overexpression improves premature ovarian failure by upregulating the expression of Notch‑1.
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December-2018
Volume 16 Issue 6

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Zhao W and Dong L: Long non‑coding RNA HOTAIR overexpression improves premature ovarian failure by upregulating Notch‑1 expression. Exp Ther Med 16: 4791-4795, 2018.
APA
Zhao, W., & Dong, L. (2018). Long non‑coding RNA HOTAIR overexpression improves premature ovarian failure by upregulating Notch‑1 expression. Experimental and Therapeutic Medicine, 16, 4791-4795. https://doi.org/10.3892/etm.2018.6750
MLA
Zhao, W., Dong, L."Long non‑coding RNA HOTAIR overexpression improves premature ovarian failure by upregulating Notch‑1 expression". Experimental and Therapeutic Medicine 16.6 (2018): 4791-4795.
Chicago
Zhao, W., Dong, L."Long non‑coding RNA HOTAIR overexpression improves premature ovarian failure by upregulating Notch‑1 expression". Experimental and Therapeutic Medicine 16, no. 6 (2018): 4791-4795. https://doi.org/10.3892/etm.2018.6750