BMP9 overexpressing adipose‑derived mesenchymal stem cells promote cartilage repair in osteoarthritis-affected knee joint via the Notch1/Jagged1 signaling pathway
- Authors:
- Published online on: September 18, 2018 https://doi.org/10.3892/etm.2018.6754
- Pages: 4623-4631
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Copyright: © Liu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
Abstract
Introduction
Osteoarthritis (OS) is a common disease in orthopedics with a high incidence in the middle-aged and elderly individuals (1). Pathologically, it is mainly characterized by loss of cartilage and degenerative changes (2). Cartilage tissue has a poor self-repair ability due to insufficient blood supply and lack of lymphatic vessel and nerve tissue distribution. The main symptoms of OS are pain and limited mobility, which affect a patient's normal life and place a heavy burden on families and society. Artificial joint replacement is a relatively effective treatment method of OS (3). To a certain degree, it eases patient's pain, but this surgery yields many short-term and long-term complications such as severe trauma, high costs, potential infection, loosening and sinking of joint implant. Cartilage transplantation, periosteal transplantation, and chondrocyte transplantation have not achieved satisfactory therapeutic effects, and there are many shortcomings such as limited donor source, immune rejection, and potential implant degradation. Therefore, their clinical application has remained limited. It is imperative to find a more simple and effective treatment.
Mesenchymal stem cells (MSCs), which are derived from the mesoderm, possess multi-directional differentiation potential (4). Under different induction conditions, MSCs can differentiate into osteoblasts (5), chondroblasts (6), and adipocytes (7). MSCs have been widely used in tissue engineering and regenerative medicine (8,9). A previous study has demonstrated that intra-articular injection of MSCs can repair cartilage defects in OS patients (10). Adipose-derived MSCs (ADMSCs) have gradually become a new generation of cells for tissue engineering because they possess the advantages of abundant donor source and being easily accessible over bone marrow-derived MSCs (BMMSCs) as well as no concerns about the ethical issue present in use of embryonic stem cells or harvesting difficulty like that encountered in obtaining BMMSCs. MSCs differentiation into articular chondrocytes is the key to treatment of cartilage defects in OS. Chondrogenic differentiation of MSCs contributes to repair of cartilage defects.
Bone morphogenetic proteins (BMPs), belonging to transforming growth factor-β (TGF-β) superfamily, is named because of their ability to induce the formation of bone and cartilage. More than 20 BMPs have been known, in which 15 BMPs are human BMPs (11). BMP2, BMP4 and BMP7 exhibit a strong ability to induce osteogenesis, and BMP2 and BMP7 have been widely used in the clinic (12–14). However, BMP2 and BMP7 induced non-fusion spine occasionally occur in the clinic (15,16). BMP9, also known as growth differentiation factor 2 (GDF-2), is the protein with the strongest ability to induce chondrogenic differentiation among the BMPs family (17). BMP9 can be obtained from the liver of mice (18). In addition to inducing chondrogenic differentiation, BMP9 also has the ability to induce and maintain cholinergic differentiation of embryonic neurons (19), inhibit the production of hepatic glucose, promote the metabolism of fatty acids (20), stimulate hepcidin-1 expression, and thereby regulate iron homeostasis in vivo (21). BMP9 exhibits a variety of biological functions and has been widely concerned because of its induction of chondrogenic differentiation. However, the mechanism by which BMP9 induces chondrogenic differentiation remains poorly understood. Whether BMP9 can be used as a cytokine for bone regeneration remains to be a hot issue.
Many signaling pathways are involved in the process of chondrogenic differentiation of ADMSCs, such as the Notch signaling pathway, Wnt signaling pathway, and TGF-β signaling pathway (22–24). The Notch signaling pathway is closely related to ADMSCs differentiation and organ formation and it is a key regulator of cell fate. There is evidence that during embryogenesis, the Notch signaling pathway is essential for the development of limb cartilage and bone (25). The Notch signaling pathway is also involved in the chondrogenict differentiation of MSCs in vitro (26). But the precise mechanism remains unclear. The majority of previous studies mainly investigated the change of the Notch signaling pathway during chondrogenic differentiation (27). There are no studies on actively regulating the Notch signaling pathway for chondrogenic differentiation of MSCs. At the same time, the mutual effects of BMP9 and the Notch signaling pathway in the chondrogenic differentiation of ADMSCs are unclear. In this study, we investigated the mutual effects of BMP9 and the Notch signaling pathway during the chondrogenic differentiation of ADMSCs through regulating BMP9 expression and the Notch signaling pathway. We also established mouse models of OS to investigate the role of BMP9 and the Notch signaling pathway in the repair of cartilage in OS affected knee joint using ADMSCs.
Materials and methods
Animals and cells
Female Balb/c mice, weighing 23–25 g, aged 8 weeks, were purchased from Beijing Vital River Laboratory Animal Technology Co., Ltd. (Beijing, China). Mouse were raised a normal diet and water, raising conditions: ambient temperature of 20–26°C, relative humidity of 40–70%, alternating day and night time of 12/12 h. Mouse ADMSCs were purchased from Cyagen Biological Technology Co., Ltd. (Taicang, China; MUBMD-01001). Animal experiments were performed in Department of Laboratory Animals, General Hospital of Shenyang Military Region (Shenyang, China; license no. SYXK2015002). The experiments were approved by Animal Ethics Committee of General Hospital of Shenyang Military Region (no. 2015049).
Groups
Mice were randomly divided into five groups, with eight mice in each group: Sham, OS, OS + ADMSCs (MSCs), OS + ADMSCs + BMP9 (BMP9), and OS + LY411575 + ADMSCs + BMP9 (LY) groups. In the sham group, mice were not subjected to any procedure. In the OS group, OS was induced. In the MSCs group, OS was induced, and ADMSCs were injected into the articular cavity. In the BMP9 group, OS was induced, and BMP9 overexpressing ADMSCs were injected into the articular cavity. In the LY group, OS was induced, and LY411575 (1.5 µmol/l) (28,29) was injected into the articular cavity to inhibit the Notch signaling pathway, and BMP9 overexpressing ADMSCs were also injected.
In the cell experiment, ADMSCs were divided into four groups: Control group (only ADMSCs), induced group (induced chondrogenic differentiation of ADMSCs), BMP9 group (chondrogenic induced ADMSCs overexpress BMP9), and LY411575 (LY) group (the Notch signaling pathway was inhibited by LY411575 (ab142164; Abcam, Cambridge, UK) at a final concentration of 1 nM (30), and chondrogenic induced ADMSCs overexpressed BMP9).
Chondrogenic induction of ADMSCs
ADMSCs in the logarithmic growth phase were digested. Cell suspension at a density of 1×105/l was seeded into a 6-well plate. Chondrogenic medium DMEM (C11885500BT; Gibco; Thermo Fisher Scientific, Inc., Waltham, MA, USA) supplemented with 10 ng/ml TGF-β1, 50 nM vitamin C, 6.25 mg/l insulin, and 10% fetal bovine serum (SH30068.03; Hyclone; GE Healthcare Life Sciences, Logan, UT, USA) was added and refreshed every 2 days. After 3 days of culture, cells were collected. Type II collagen and aggrecan expression in the chondrogenic induced ADMSCs was detected by PCR and western blot assay.
BMP9-transfected ADMSCs
ADMSCs in the logarithmic growth phase at a final concentration of 1×105/l were cultured with DMEM supplemented with pVSV-G-BMP9 (GenePharma Co., Ltd., Shanghai, China) and Polybrene (5 µg/ml). After 12 h, culture medium was refreshed and cells were cultured for 2 more days. Cells were observed under fluorescence microscope. BMP9-transfected ADMSCs were identified by real-time fluorescence quantitative PCR method.
Establishment of mouse models of OS
Mouse models of OS were established by transection of the knee anterior cruciate ligament (31). Precisely, after anesthesia, an anesthesia ventilataor was used. The flow rate of isoflurane was adjusted. A medial patellar incision was made and the skin and articular capsule were cut open. The patella was laterally retracted. The knee was flexed as far as possible to expose the anterior cruciate ligament and the anterior horn of the medial meniscus. The bilateral anterior cruciate ligament was transected. The anterior drawer experiment was performed to confirm that the bilateral anterior cruciate ligament was completely cut off. Caution should be made to protect articular cartilage surface. The articular space was flushed with normal saline. Articular capsule and skin were sutured layer by layer.
Intra-articular injection of ADMSCs
ADMSCs were digested and prepared into cell suspension (at a cell density of 1×107/ml) using chondrogenic medium DMEM (C11885500BT; Gibco; Thermo Fisher Scientific, Inc.). Four weeks after OS induction, ADMSCs were injected into the articular capsule once a week. Precisely, after anesthesia, a 2 mm-long lateral incision was made on the lower limb. The knee was touched by stretching the skin. Cell suspension was injected into the articular cavity via the midpoint of the medial edge of the ligament. 10 µl ADMSCs per articular cavity was injected. After needle withdrawal, the entry site was slightly pressurized to prevent the overflow of cell suspension. Skin incision was sutured.
H&E staining
Four weeks after intra-articular injection of ADMSCs, knee joint was disarticulated and fixed with formalin. The harvested tissue sample was de-calcified, dehydrated and embedded with paraffin. Sample tissues were de-waxed, rehydrated, stained with hematoxylin for 5 min, washed with PBS, differentiated with hydrochloric acid ethanol for 3 sec, stained with eosin for 3 min, dehydrated, cleared, mounted with neutral resin, and finally observed under the optical microscope (NE950; Leica Microsystems, Inc., Buffalo Grove, IL, USA).
Toluidine blue staining
Tissue sections (5 µm) were de-waxed by xylene, rehydrated, and stained with 0.1% toluidine blue for 10 min at room temperature, washed with PBS three times, differentiated with glacial acetic acid, dehydrated in ethanol gradients, cleared with clear liquid, dried, and mounted with neutral gum.
Western blot analysis
Total protein was extracted from ADMSCs and knee tissue samples in different groups. Protein concentration was determined using a BCA protein assay kit (23227; Thermo Fisher Scientific, Inc.). Protein samples were subjected to SDS-PAGE and then transferred to a PVDF membrane. After addition of type II collagen (ab34712), aggrecan (ab3778), notch1 (ab52627), Jagged1 (ab7771), and GAPDH (ab181602; all Abcam) protein samples were incubated at 4°C overnight and washed with PBS. After addition of secondary antibody (goat anti-rabbit IgG/HRP antibody; 1:2,000; Bioss, Beijing, China), protein samples were incubated at 37°C for 2 h. Protein bands were visualized using an ECL chemiluminescence detection kit (32109; Thermo Fisher Scientific, Inc.) and a gel imaging system (ChemiDoc MP; Bio-Rad Laboratories, Inc., Hercules, CA, USA). Absorbance analysis was performed using Image J software (Image J 1.8.0; National Institutes of Health, Bethesda, MD, USA).
Reverse transcription-quantitative polymerase chain reaction (RT-qPCR)
Primers were designed according to the sequences of BMP9, type II collagen, aggrecan reported in Genbank, and were synthesized in Shanghai Biomedical Biotechnology Co., Ltd. Total RNA was isolated with TRIzol reagent (15596018), and reversely transcribed into cDNA (4387406; both Invitrogen; Thermo Fisher Scientific, Inc.). Real-time PCR kit (RR820A; Takara Biotechnology Co., Ltd., Dalian, China) was used for the detection. The relative gene expression data was analyzed with the 2−ΔΔCq method (32). The primers used for real-time PCR were listed as follows: BMP9 forward, GCTGCAGAACTGGGAACA and reverse, AACAAGCATCCCCTGGGG; Collagen II forward, TGCTGGCCCAACTGGCAA and reverse, ATTGTTGGTCTGCCTGGT; Aggrecan forward, CCAGTGAGGACCTGGTAGTG and reverse, CAGGCCTGCATGCACACCG; Notch1 forward, AAGAGGCTTGAGATGCTCC and reverse, TGCCTCAGCACACCGTGT; Jagged1 forward, TAACACCTTCAATCTCAAG and reverse, ATGACACTATTCAACCTGA; GAPDH forward, GAATCGATCCATACTTATC and reverse, CCTTGAAGATATGGGCAC.
Statistical analysis
All data were statistically analyzed using SPSS v 19.0 software (IBM Corp., Armonk, NY, USA). A Kolmogorov-Smirnov (K-S) test was used to determine whether data were normally distributed. If data were normally distributed, the data were presented as mean ± SEM. One-way ANOVA followed by a Student-Newman-Keuls test was used to test for differences among more than two groups. A level of P<0.05 was considered to indicate a statistically significant difference.
Results
Chondrogenic induction of ADMSCs
To determine the differentiation of ADMSCs into chondrocytes, as confirmed by western blot assay (Fig. 1A) and RT-qPCR (Fig. 1B), after chondrogenic induction of ADMSCs, type II collagen and aggrecan expression levels were significantly increased in induced group (P<0.05 vs. control group), indicating that ADMSCs can be induced toward chondrogenic differentiation.
BMP9 promoted chondrogenic differentiation of ADMSCs
To determine the effect of BMP9, we performed BMP9 overexpression. At 72 h after pVSV-G-BMP9 lentivirus-transfected ADMSCs were directed toward chondrogenic differentiation, RT-qPCR was performed to detect intracellular BMP9 expression. After BMP9 transfection, intracellular BMP9 expression was significantly increased (P<0.05; Fig. 2A and B), this result proved that our transfection was successful. We further detected the expression of type II collagen and aggrecan using western blot assay (Fig. 2C) and RT-qPCR (Fig. 2D). The results showed that type II collagen and aggrecan expression was significantly increased in BMP9-transfected ADMSCs than that in the chondrogenic induced ADMSCs (P<0.05). These results suggest that BMP9 promoted chondrogenic differentiation of ADMSCs possibly through the Notch signaling pathway.
BMP9 regulated the Notch1/Jagged1 signaling pathway to promote the chondrogenic differentiation of ADMSCs
In order to further explore the mechanism of BMP9, western blot assay was performed to detect the expression of Notch1/Jagged1 signaling pathway-related proteins Notch1 and Jagged1. After BMP9 was overexpressed, Notch1 and Jagged1 expression was significantly decreased than that in the chondrogenic induced ADMSCs (P<0.05; Fig. 3A). This was consistent with RT-qPCR findings (Fig. 3B). These results confirm that BMP9 regulated chondrogenic differentiation of ADMSCs through the Notch1/Jagged1 signaling pathway.
ADMSCs promoted cartilage repair in OS affected joints in mice
H&E staining results (Fig. 4) showed that in the OS group, articular chondrocytes were poorly arranged, and their number was smaller than that in sham group, and cartilage was thinner than that in the control group. Toluidine blue staining (Fig. 4B) showed that in the OS group, proteoglycan was unevenly distributed. After intra-articular injection of BMP9 overexpressing ADMSCs, the number of chondrocytes in the articular cavity was increased, and cartilage was thickened. In order to further explore the role of BMP9 in mice, western blot assay (Fig. 4C) was performed to detect intra-articular expression of type II collagen and aggrecan protein. The results showed that after BMP9 overexpression, type II collagen and aggrecan expression were significantly increased. When the Notch signaling pathway in the ADMSCs was inhibited, type II collagen and aggrecan expression were significantly decreased. This was consistent with RT-qPCR findings (Fig. 4D). These results demonstrate that BMP9 overexpressing ADMSCs can promote the healing of OS in mice.
BMP9 regulated the Notch/Jagged1 signaling pathway in ADMSCs to promote OS healing in mice
To further validate whether BMP9 overexpressing ADMSCs function effect via the Notch1/Jagged1 signaling pathway, western blot assay was performed to detect intra-articular expression of notch1 and Jagged1. In the BMP9 group, intra-articular expression of Notch1 and Jagged1 was significantly higher than that in the OS group (P<0.05) (Fig. 5A). When the Notch signaling pathway was inhibited, type II collagen and aggrecan expression was decreased (Fig. 4C and D) and Jagged1 expression was also significantly reduced (P<0.05 vs. BMP9 group). This was supported by RT-qPCR findings (Fig. 5B). These results confirmed that BMP9 overexpressing ADMSCs promote OS healing in mice through the Notch/Jagged1 signaling pathway.
Discussion
Stem cells are mainly obtained from skeletal muscle satellite cells, embryonic stem cells, and bone marrow MSCs (BMMSCs). It is difficult to obtain skeletal muscle satellite cells because of its lower level. Embryonic stem cells have immunological rejections and ethical issues. BMMSCs also have the shortcomings of difficult harvesting and that patients are not willing to accept.
Zuk et al (33) were the first to harvest ADMSCs with multi-directional differentiation potential from adipose tissue suspension during human liposuction and induced them to differentiate into adipocytes, chondroblasts, osteoblasts, and neural progenitors. ADMSCs are easily accepted because of easily accessible rich resource, being able to rapidly proliferate in any kind of serum, no immunological considerations during autografting, and no ethical issues. At present, there are no specific surface markers of ADMSCs. Gronthos and Zannettino (34) cultured cells of fat tissue aspirates, systematically studied cell surface markers, and found that ADMSCs were spindle-shaped, had abundant cytoplasm and nucleoli, and grew in a parallel or spiral-like manner. These cells have surface markers, including CD59, CD105, CD106, CD146 and CD165, which are similar to BMMSCs (35). But STRO-1 antigen was not detected on ADMSCs.
Festy et al (36) found that ADMSCs are similar to fully differentiated adipocyte surface markers, and the surface markers are not different between subcutaneous ADMSCs and omental ADMSCs. In the experiments, ADMSCs were isolated. Cell surface markers CD13, CD44 and CD59 on ADMSCs were detected. Flow cytometry showed that CD13, CD44, and CD59 were positive, indicating that the harvested ADMSCs are highly purified. ADMSCs, as a kind of multi-potential stem cells, share the features with stem cells, that is to say, ADMSCs theoretically have the ability to infinitely proliferate.
BMP9, as a less studied BMP, has a very strong potential for chondrogenesis. There is evidence that BMP9 together with the Notch signaling pathway plays an important role in embryonic development, cell proliferation and differentiation, and the occurrence of diseases (37). In the early study, BMP9 and the Notch signaling were found to have synergistic effects in the early stage of osteogenesis (38). However, the precise mechanism remains poorly understood. This is the problem that needs to be studied in this paper.
In this study, we overexpressed BMP9 in ADMSCs, observed change in cell masses under the inverted microscope, and detected cartilage type II collagen and aggrecan expression. Results showed that upregulating BMP9 expression can further induce chondrogenic differentiation of ADMSCs. To further clarify the mutual effects of the Notch signaling pathway and BMP9 in the chondrogenic differentiation of ADMSCs, we used LY411575 to inhibit the Notch signaling pathway. Inhibiting the Notch signaling pathway using LY411575 can inhibit the chondrogenic differentiation of ADMSCs, confirming that the Notch signaling pathway can inhibit the chondrogenic differentiation of ADMSCs.
After intra-articular injection of ADMSCs, a larger degree of cartilage repair was found in the MSCs group than in the control group. After intra-articular injection of BMP9 overexpressing ADMSCs, type II collagen and aggrecan protein expression in the cartilage of OS affected knee joint was further detected by immunohistochemical staining to further confirm the mechanism underlying BMP9-overexpressing ADMSCs. BMP9 overexpressing ADMSCs were injected into the articular cavity to inhibit the Notch signaling pathway. Results showed that ADMSCs promoted OS healing in mice through the Notch1/Jagged1 signaling pathway.
Taken together, ADMSCs express multiple stem cell surface markers and can be induced to differentiate into chondrocytes, confirming that ADMSCs exhibit multi-directional differentiation potential. Upregulating BMP9 protein can promote the chondrogenic differentiation of ADMSCs. Inhibition of the Notch signaling pathway can inhibit the chondrogenic differentiation of ADMSCs. Intra-articular injection of ADMSCs contributes to cartilage repair OS affected knee joint in mice, and the repair is achieved via the Notch1/Jagged1 signaling pathway.
Acknowledgements
Not applicable.
Funding
The present study was supported by a grant from the Liaoning Province Science and Technology Issues in China (grant no. 2013225089).
Availability of data and materials
The datasets used and/or analyzed during the current study are available from the corresponding author on reasonable request.
Authors' contributions
XinL and XiaL conceived and designed the study, acquired data, interpreted the results and drafted the manuscript. XiaL also contributed to acquisition of funding support. MD, YW and SL performed the experiments. XinL and XiaL analyzed the data. All authors read and approved the final manuscript.
Ethics approval and consent to participate
The experiments were approved by Animal Ethics Committee of General Hospital of Shenyang Military Region, China (no. 2015049).
Patient consent for publication
Not applicable.
Competing interests
The authors declare that they have no competing interests.
Glossary
Abbreviations
Abbreviations:
BMP9 |
bone morphogenetic protein-9 |
OS |
osteoarthritis |
MSCs |
mesenchymal stem cells |
ADMSCs |
adipose-derived mesenchymal stem cells |
BMMSCs |
bone marrow mesenchymal stem cells |
TGF-β |
transforming growth factor-β |
GDF-2 |
growth differentiation factor 2 |
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