Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Experimental and Therapeutic Medicine
Join Editorial Board Propose a Special Issue
Print ISSN: 1792-0981 Online ISSN: 1792-1015
Journal Cover
December-2018 Volume 16 Issue 6

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
December-2018 Volume 16 Issue 6

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article Open Access

Cellular viability and osteogenic differentiation potential of human gingiva‑derived stem cells in 2D culture following treatment with anionic, cationic, and neutral liposomes containing doxorubicin

  • Authors:
    • Hyunjin Lee
    • Jihwan Son
    • Gawon Yi
    • Heebeom Koo
    • Jun‑Beom Park
  • View Affiliations / Copyright

    Affiliations: Department of Periodontics, College of Medicine, The Catholic University of Korea, Seoul 06591, Republic of Korea, Department of Medical Lifescience, College of Medicine, The Catholic University of Korea, Seoul 06591, Republic of Korea
    Copyright: © Lee et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 4457-4462
    |
    Published online on: September 19, 2018
       https://doi.org/10.3892/etm.2018.6777
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

The effects of doxorubicin, particularly doxorubicin liposome, on stem cells have remained to be fully elucidated. The aim of the present study was to evaluate the effects of anionic, cationic and neutral liposomes loaded with doxorubicin on the viability and osteogenic differentiation potential of human gingiva‑derived stem cells in two‑dimensional culture. Doxorubicin‑loaded liposomes were prepared using the traditional thin‑lipid‑film hydration method. Stem cells were seeded on a culture plate and maintained in osteogenic media. The morphology of the stem cells was observed under an inverted microscope. The number of viable cells was determined using a Cell‑Counting Kit‑8 assay. The alkaline phosphatase activity was assessed and Alizarin Red S staining was performed to evaluate osteogenic differentiation. A higher concentration of doxorubicin caused noticeable changes in the morphology of the stem cells. Decreases in cellular viability were observed after applying doxorubicin. The application of doxorubicin, particularly at higher concentrations, produced a noticeable decrease in alkaline phosphatase activity and Alizarin Red S staining. The present study indicated that application of doxorubicin with or without liposomes reduced the cellular viability and osteogenic differentiation. Among the different treatments, the doxorubicin‑loaded cationic liposomes induced the strongest reduction in the cellular viability and osteogenic differentiation in the stem cell culture.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

Figure 6

Figure 7

Figure 8

Figure 9

View References

1 

Ruttala HB, Ramasamy T, Gupta B, Choi HG, Yong CS and Kim JO: Multiple polysaccharide-drug complex-loaded liposomes: A unique strategy in drug loading and cancer targeting. Carbohydr Polym. 173:57–66. 2017. View Article : Google Scholar : PubMed/NCBI

2 

Bagnyukova TV, Serebriiskii IG, Zhou Y, Hopper-Borge EA, Golemis EA and Astsaturov I: Chemotherapy and signaling: How can targeted therapies supercharge cytotoxic agents? Cancer Biol Ther. 10:839–853. 2010. View Article : Google Scholar : PubMed/NCBI

3 

Zhang Y, Zhai M, Chen Z, Han X, Yu F, Li Z, Xie X, Han C, Yu L, Yang Y and Mei X: Dual-modified liposome codelivery of doxorubicin and vincristine improve targeting and therapeutic efficacy of glioma. Drug Deliv. 24:1045–1055. 2017. View Article : Google Scholar : PubMed/NCBI

4 

Rana T, Chakrabarti A, Freeman M and Biswas S: Doxorubicin-mediated bone loss in breast cancer bone metastases is driven by an interplay between oxidative stress and induction of TGFbeta. PLoS One. 8:e780432013. View Article : Google Scholar : PubMed/NCBI

5 

Affram K, Udofot O, Singh M, Krishnan S, Reams R, Rosenberg J and Agyare E: Smart thermosensitive liposomes for effective solid tumor therapy and in vivo imaging. PLoS One. 12:e01851162017. View Article : Google Scholar : PubMed/NCBI

6 

Fukuda A, Tahara K, Hane Y, Matsui T, Sasaoka S, Hatahira H, Motooka Y, Hasegawa S, Naganuma M, Abe J, et al: Comparison of the adverse event profiles of conventional and liposomal formulations of doxorubicin using the FDA adverse event reporting system. PLoS One. 12:e01856542017. View Article : Google Scholar : PubMed/NCBI

7 

Lee H, Son J, Na CB, Yi G, Koo H and Park JB: The effects of doxorubicin-loaded liposomes on viability, stem cell surface marker expression and secretion of vascular endothelial growth factor of three-dimensional stem cell spheroids. Exp Ther Med. 15:4950–4960. 2018.PubMed/NCBI

8 

Jin SH, Lee JE, Yun JH, Kim I, Ko Y and Park JB: Isolation and characterization of human mesenchymal stem cells from gingival connective tissue. J Periodontal Res. 50:461–467. 2015. View Article : Google Scholar : PubMed/NCBI

9 

Contrera JF, Matthews EJ, Kruhlak NL and Benz RD: Estimating the safe starting dose in phase I clinical trials and no observed effect level based on QSAR modeling of the human maximum recommended daily dose. Regul Toxicol Pharmacol. 40:185–206. 2004. View Article : Google Scholar : PubMed/NCBI

10 

Niu H, Xu M, Li S, Chen J, Luo J, Zhao X, Gao C and Li X: High-performance liquid chromatography (HPLC) quantification of liposome-delivered doxorubicin in arthritic joints of collagen-induced arthritis rats. Med Sci Monit Basic Res. 23:150–158. 2017. View Article : Google Scholar : PubMed/NCBI

11 

Daeihamed M, Haeri A, Ostad SN, Akhlaghi MF and Dadashzadeh S: Doxorubicin-loaded liposomes: Enhancing the oral bioavailability by modulation of physicochemical characteristics. Nanomedicine (Lond). 12:1187–1202. 2017. View Article : Google Scholar : PubMed/NCBI

12 

Xu H, Zhang L, Li L, Liu Y, Chao Y, Liu X, Jin Z, Chen Y, Tang X, He H, et al: Membrane-loaded doxorubicin liposomes based on ion-pairing technology with high drug loading and pH-responsive property. AAPS PharmSciTech. 18:2120–2130. 2017. View Article : Google Scholar : PubMed/NCBI

13 

Moitra P, Kumar K, Sarkar S, Kondaiah P, Duan W and Bhattacharya S: New pH-responsive gemini lipid derived co-liposomes for efficacious doxorubicin delivery to drug resistant cancer cells. Chem Commun (Camb). 53:8184–8187. 2017. View Article : Google Scholar : PubMed/NCBI

14 

Lee S, Lee SY, Park S, Ryu JH, Na JH, Koo H, Lee KE, Jeon H, Kwon IC, Kim K and Jeong SY: In vivo NIRF imaging of tumor targetability of nanosized liposomes in tumor-bearing mice. Macromol Biosci. 12:849–856. 2012. View Article : Google Scholar : PubMed/NCBI

15 

Sarfraz M, Afzal A, Raza SM, Bashir S, Madni A, Khan MW, Ma X and Xiang G: Liposomal co-delivered oleanolic acid attenuates doxorubicin-induced multi-organ toxicity in hepatocellular carcinoma. Oncotarget. 8:47136–47153. 2017. View Article : Google Scholar : PubMed/NCBI

16 

Sreekanth V, Medatwal N, Pal S, Kumar S, Sengupta S and Bajaj A: Molecular self-assembly of bile acid-phospholipids controls the delivery of doxorubicin and mice survivability. Mol Pharm. 14:2649–2659. 2017. View Article : Google Scholar : PubMed/NCBI

17 

Razavi-Azarkhiavi K, Jaafari MR, Abnous K, Razavi BM, Jafarian AH, Hassani FV, Shirani K and Karimi G: The cardiotoxic mechanism of doxorubicin (DOX) and pegylated liposomal DOX in mice bearing C-26 colon carcinoma: A study focused on microRNA role for toxicity assessment of new formulations. Pharm Res. 34:1849–1856. 2017. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Lee H, Son J, Yi G, Koo H and Park JB: Cellular viability and osteogenic differentiation potential of human gingiva‑derived stem cells in 2D culture following treatment with anionic, cationic, and neutral liposomes containing doxorubicin. Exp Ther Med 16: 4457-4462, 2018.
APA
Lee, H., Son, J., Yi, G., Koo, H., & Park, J. (2018). Cellular viability and osteogenic differentiation potential of human gingiva‑derived stem cells in 2D culture following treatment with anionic, cationic, and neutral liposomes containing doxorubicin. Experimental and Therapeutic Medicine, 16, 4457-4462. https://doi.org/10.3892/etm.2018.6777
MLA
Lee, H., Son, J., Yi, G., Koo, H., Park, J."Cellular viability and osteogenic differentiation potential of human gingiva‑derived stem cells in 2D culture following treatment with anionic, cationic, and neutral liposomes containing doxorubicin". Experimental and Therapeutic Medicine 16.6 (2018): 4457-4462.
Chicago
Lee, H., Son, J., Yi, G., Koo, H., Park, J."Cellular viability and osteogenic differentiation potential of human gingiva‑derived stem cells in 2D culture following treatment with anionic, cationic, and neutral liposomes containing doxorubicin". Experimental and Therapeutic Medicine 16, no. 6 (2018): 4457-4462. https://doi.org/10.3892/etm.2018.6777
Copy and paste a formatted citation
x
Spandidos Publications style
Lee H, Son J, Yi G, Koo H and Park JB: Cellular viability and osteogenic differentiation potential of human gingiva‑derived stem cells in 2D culture following treatment with anionic, cationic, and neutral liposomes containing doxorubicin. Exp Ther Med 16: 4457-4462, 2018.
APA
Lee, H., Son, J., Yi, G., Koo, H., & Park, J. (2018). Cellular viability and osteogenic differentiation potential of human gingiva‑derived stem cells in 2D culture following treatment with anionic, cationic, and neutral liposomes containing doxorubicin. Experimental and Therapeutic Medicine, 16, 4457-4462. https://doi.org/10.3892/etm.2018.6777
MLA
Lee, H., Son, J., Yi, G., Koo, H., Park, J."Cellular viability and osteogenic differentiation potential of human gingiva‑derived stem cells in 2D culture following treatment with anionic, cationic, and neutral liposomes containing doxorubicin". Experimental and Therapeutic Medicine 16.6 (2018): 4457-4462.
Chicago
Lee, H., Son, J., Yi, G., Koo, H., Park, J."Cellular viability and osteogenic differentiation potential of human gingiva‑derived stem cells in 2D culture following treatment with anionic, cationic, and neutral liposomes containing doxorubicin". Experimental and Therapeutic Medicine 16, no. 6 (2018): 4457-4462. https://doi.org/10.3892/etm.2018.6777
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team