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Article

Regulatory role of miRNA‑26a in neonatal sepsis

  • Authors:
    • Qi Cheng
    • Lili Tang
    • Yibiao Wang
  • View Affiliations / Copyright

    Affiliations: Department of Pediatrics, The Second Hospital of Shandong University, Jinan, Shandong 250033, P.R. China, Department of Pediatrics, Gaotang County People's Hospital, Liaocheng, Shandong 252800, P.R. China, Department of Pediatrics, The Second Hospital of Shandong University, Jinan, Shandong 250000, P.R. China
  • Pages: 4836-4842
    |
    Published online on: September 19, 2018
       https://doi.org/10.3892/etm.2018.6779
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Abstract

The present study aimed to investigate the expression of microRNA (miRNA) 26a in blood mononuclear cells and serum in neonatal sepsis, as well as its role in the disease pathogenesis. In total 28 cases of neonatal sepsis were included in the study. The mRNA expression levels of miRNA‑26a and interleukin (IL)‑6 in the blood mononuclear cells and serum samples were detected by reverse transcription‑quantitative polymerase chain reaction. The protein expression of IL‑6 was detected by western blot analysis and ELISA. The in vitro septic environment was simulated by lipopolysaccharide (LPS) in THP‑1 cells, and the expression of miRNA‑26a and IL‑6 were determined. Interaction between miRNA‑26a and IL‑6 was confirmed by a dual‑luciferase reporter assay. Compared with the control group, the mRNA and protein expression levels of IL‑6 in the blood mononuclear cells and serum samples from the neonates with sepsis were significantly elevated, while the expression of miRNA‑26a was significantly decreased. In addition, similar results were observed in the LPS‑induced septic models in THP‑1 cells. Furthermore, the results of the dual‑luciferase reporter assay demonstrated that IL‑6 was the direct target of miRNA‑26a. The expression of IL‑6 was significantly upregulated in the blood mononuclear cells and serum in neonatal sepsis, which may be associated with the downregulation of miRNA‑26a. miRNA‑26a may regulate the disease pathogenesis and immune responses.
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Copy and paste a formatted citation
Spandidos Publications style
Cheng Q, Tang L and Wang Y: Regulatory role of miRNA‑26a in neonatal sepsis. Exp Ther Med 16: 4836-4842, 2018.
APA
Cheng, Q., Tang, L., & Wang, Y. (2018). Regulatory role of miRNA‑26a in neonatal sepsis. Experimental and Therapeutic Medicine, 16, 4836-4842. https://doi.org/10.3892/etm.2018.6779
MLA
Cheng, Q., Tang, L., Wang, Y."Regulatory role of miRNA‑26a in neonatal sepsis". Experimental and Therapeutic Medicine 16.6 (2018): 4836-4842.
Chicago
Cheng, Q., Tang, L., Wang, Y."Regulatory role of miRNA‑26a in neonatal sepsis". Experimental and Therapeutic Medicine 16, no. 6 (2018): 4836-4842. https://doi.org/10.3892/etm.2018.6779
Copy and paste a formatted citation
x
Spandidos Publications style
Cheng Q, Tang L and Wang Y: Regulatory role of miRNA‑26a in neonatal sepsis. Exp Ther Med 16: 4836-4842, 2018.
APA
Cheng, Q., Tang, L., & Wang, Y. (2018). Regulatory role of miRNA‑26a in neonatal sepsis. Experimental and Therapeutic Medicine, 16, 4836-4842. https://doi.org/10.3892/etm.2018.6779
MLA
Cheng, Q., Tang, L., Wang, Y."Regulatory role of miRNA‑26a in neonatal sepsis". Experimental and Therapeutic Medicine 16.6 (2018): 4836-4842.
Chicago
Cheng, Q., Tang, L., Wang, Y."Regulatory role of miRNA‑26a in neonatal sepsis". Experimental and Therapeutic Medicine 16, no. 6 (2018): 4836-4842. https://doi.org/10.3892/etm.2018.6779
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