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Dysregulated microRNA expression profiles in gastric cancer cells with high peritoneal metastatic potential

  • Authors:
    • Yaning Feng
    • Feihu Bai
    • Yanjie You
    • Fangyun Bai
    • Chuanxia Wu
    • Ruijuan Xin
    • Xue Li
    • Yongzhan Nie
  • View Affiliations / Copyright

    Affiliations: Department of Gastroenterology, Ningxia Hui Autonomous Region People's Hospital, Yinchuan, Ningxia Hui Autonomous Region 750021, P.R. China, Department of General Medicine, General Hospital of Ningxia Medical University, Yinchuan, Ningxia Hui Autonomous Region 750001, P.R. China, Department of Gastroenterology, The Second People's Hospital of Linyi, Linyi, Shandong 276000, P.R. China, Department of Gastroenterology, Xijing Hospital of Digestive Diseases, State Key Laboratory of Cancer Biology and Institute of Digestive Diseases, Xi'an, Shaanxi 710000, P.R. China
    Copyright: © Feng et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 4602-4608
    |
    Published online on: September 19, 2018
       https://doi.org/10.3892/etm.2018.6783
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Abstract

Despite significant developments in its clinical treatment, the reported incidence and mortality of gastric cancer have exhibited marked increases. The molecular mechanisms of gastric cancer initiation and progression remain to be fully elucidated. The aim of the present study was to identify novel microRNAs (miRNAs/miRs) with a role in the peritoneal metastasis of gastric cancer by comparing the miRNA expression in the gastric cancer cell line GC9811 with that in its variant GC9811‑P, a sub‑cell line with a high potential for peritoneal metastasis. A miRNA microarray analysis identified 153 dysregulated miRNAs, including 74 upregulated and 79 downregulated miRNAs. Of these, four significantly upregulated miRNAs (miR‑181a‑5p, miR‑106b‑5p, miR‑199a‑3p and miR‑148a‑3p) and four downregulated miRNAs (miR‑146a‑5p, miR‑21‑5p, miR‑222‑3p and miR‑221‑3p) were selected and further confirmed by reverse transcription‑quantitative polymerase chain reaction analysis. Furthermore, knockdown of miR‑21‑5p promoted the migration and invasion of GC9811 cells. Collectively, the results suggested that the miRNA expression profile in GC9811‑P vs. GC9811 cells was altered to favor disease progression, and the dysregulated miRNAs, including miR‑21‑5p, may therefore provide novel biomarkers and potential therapeutic targets for gastric cancer metastasis.
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View References

1 

Parkin DM, Bray F, Ferlay J and Pisani P: Global cancer statistics, 2002. CA Cancer J Clin. 55:74–108. 2005. View Article : Google Scholar : PubMed/NCBI

2 

Yonemura Y, Endou Y, Sasaki T, Hirano M, Mizumoto A, Matsuda T, Takao N, Ichinose M, Miura M and Li Y: Surgical treatment for peritoneal carcinomatosis from gastric cancer. Eur J Surg Oncol. 36:1131–1138. 2010. View Article : Google Scholar : PubMed/NCBI

3 

Bozzetti F, Yu W, Baratti D, Kusamura S and Deraco M: Locoregional treatment of peritoneal carcinomatosis from gastric cancer. J Surg Oncol. 98:273–276. 2008. View Article : Google Scholar : PubMed/NCBI

4 

Wightman B, Ha I and Ruvkun G: Posttranscriptional regulation of the heterochronic gene lin-14 by lin-4 mediates temporal pattern formation in C. elegans. Cell. 75:855–862. 1993. View Article : Google Scholar : PubMed/NCBI

5 

Lee RC, Feinbaum RL and Ambros V: The C. elegans heterochronic gene lin-4 encodes small RNAs with antisense complementarity to lin-14. Cell. 75:843–854. 1993. View Article : Google Scholar : PubMed/NCBI

6 

Takei Y, Takigahira M, Mihara K, Tarumi Y and Yanagihara K: The metastasis-associated microRNA miR-516a-3p is a novel therapeutic target for inhibiting peritoneal dissemination of human scirrhous gastric cancer. Cancer Res. 71:1442–1453. 2011. View Article : Google Scholar : PubMed/NCBI

7 

Nishida N, Mimori K, Fabbri M, Yokobori T, Sudo T, Tanaka F, Shibata K, Ishii H, Doki Y and Mori M: MicroRNA-125a-5p is an independent prognostic factor in gastric cancer and inhibits the proliferation of human gastric cancer cells in combination with trastuzumab. Clin Cancer Res. 17:2725–2733. 2011. View Article : Google Scholar : PubMed/NCBI

8 

Liang S, He L, Zhao X, Miao Y, Gu Y, Guo C, Xue Z, Dou W, Hu F, Wu K, et al: MicroRNA let-7f inhibits tumor invasion and metastasis by targeting MYH9 in human gastric cancer. PLoS One. 6:e184092011. View Article : Google Scholar : PubMed/NCBI

9 

Bai F, Guo X, Yang L, Wang J, Shi Y, Zhang F, Zhai H, Lu Y, Xie H, Wu K and Fan D: Establishment and characterization of a high metastatic potential in the peritoneum for human gastric cancer by orthotopic tumor cell implantation. Dig Dis Sci. 52:1571–1578. 2007. View Article : Google Scholar : PubMed/NCBI

10 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI

11 

You Y, Liu J, Wang Z, Zhang Y, Ran Y, Guo X, Liu H and Wang H: The enhancement of radiosensitivity in human esophageal squamous cell carcinoma cells by zoledronic acid and its potential mechanism. Cytotechnology. 66:17–25. 2014. View Article : Google Scholar : PubMed/NCBI

12 

You Y, Yang W, Qin X, Wang F, Li H, Lin C, Li W, Gu C, Zhang Y and Ran Y: ECRG4 acts as a tumor suppressor and as a determinant of chemotherapy resistance in human nasopharyngeal carcinoma. Cell Oncol(Dordr). 38:205–214. 2015. View Article : Google Scholar : PubMed/NCBI

13 

Tseng CW, Lin CC, Chen CN, Huang HC and Juan HF: Integrative network analysis reveals active microRNAs and their functions in gastric cancer. BMC Syst Biol. 5:992011. View Article : Google Scholar : PubMed/NCBI

14 

Liu K, LI G, Fan C, Diao Y, Wu B and LI J: Increased expression of microRNA-221 in gastric cancer and its clinical significance. J Int Med Res. 40:467–474. 2012. View Article : Google Scholar : PubMed/NCBI

15 

Kogo R, Mimori K, Tanaka F, Komune S and Mori M: Clinical significance of miR-146a in gastric cancer cases. Clin Cancer Res. 17:4277–4284. 2011. View Article : Google Scholar : PubMed/NCBI

16 

Li Y, Vandenboom TG II, Wang Z, Kong D, Ali S, Philip PA and Sarkar FH: miR-146a suppresses invasion of pancreatic cancer cells. Cancer Res. 70:1486–1495. 2010. View Article : Google Scholar : PubMed/NCBI

17 

Bhaumik D, Scott GK, Schokrpur S, Patil CK, Campisi J and Benz CC: Expression of microRNA-146 suppresses NF-kappaB activity with reduction of metastatic potential in breast cancer cells. Oncogene. 27:5643–5647. 2008. View Article : Google Scholar : PubMed/NCBI

18 

Hurst DR, Edmonds MD, Scott GK, Benz CC, Vaidya KS and Welch DR: Breast cancer metastasis suppressor 1 up-regulates miR-146, which suppresses breast cancer metastasis. Cancer Res. 69:1279–1283. 2009. View Article : Google Scholar : PubMed/NCBI

19 

Lin SL, Chiang A, Chang D and Ying SY: Loss of mir-146a function in hormone-refractory prostate cancer. RNA. 14:417–424. 2008. View Article : Google Scholar : PubMed/NCBI

20 

Magrelli A, Azzalin G, Salvatore M, Viganotti M, Tosto F, Colombo T, Devito R, Di Masi A, Antoccia A, Lorenzetti S, et al: Altered microRNA expression patterns in hepato-blastoma patients. Transl Oncol. 2:157–163. 2009. View Article : Google Scholar : PubMed/NCBI

21 

Song G, Zeng H, LI J, Xiao L, He Y, Tang Y and Li Y: miR-199a regulates the tumor suppressor mitogen-activated protein kinase 11 in gastric cancer. Biol Pharm Bull. 33:1822–1827. 2010. View Article : Google Scholar : PubMed/NCBI

22 

Yang H, Kong W, He L, Zhao JJ, O'Donnell JD, Wang J, Wenham RM, Coppola D, Kruk PA, Nicosia SV and Cheng JQ: MicroRNA expression profling in human ovarian cancer: miR-214 induces cell survival and cisplatin resistance by targeting PTEN. Cancer Res. 68:425–433. 2008. View Article : Google Scholar : PubMed/NCBI

23 

Ichimi T, Enokida H, Okuno Y, Kunimoto R, Chiyomaru T, Kawamoto K, Kawahara K, Toki K, Kawakami K, Nishiyama K, et al: Identifcation of novel microRNA targets based on microRNA signatures in bladder cancer. Int J Cancer. 125:345–352. 2009. View Article : Google Scholar : PubMed/NCBI

24 

Feng YH and Tsao CJ: Emerging role of microRNA-21 in cancer. Biomed Rep. 5:395–402. 2016. View Article : Google Scholar : PubMed/NCBI

25 

Motoyama K, Inoue H, Mimori K, Tanaka F, Kojima K, Uetake H, Sugihara K and Mori M: Clinicopathological and prognostic significance of PDCD4 and microRNA-21 in human gastric cancer. Int J Oncol. 36:1089–1095. 2010.PubMed/NCBI

26 

Ou H, Li Y and Kang M: Activation of miR-21 by STAT3 induces proliferation and suppresses apoptosis in nasopharyngeal carcinoma by targeting PTEN gene. PLoS One. 9:e1099292014. View Article : Google Scholar : PubMed/NCBI

27 

Meng F, Henson R, Wehbe-Janek H, Ghoshal K, Jacob ST and Patel T: MicroRNA-21 regulates expression of the PTEN tumor suppressor gene in human hepatocellular cancer. Gastroenterology. 133:647–658. 2007. View Article : Google Scholar : PubMed/NCBI

28 

Wei X, Wang W, Wang L, Zhang Y, Zhang X, Chen M, Wang F, Yu J, Ma Y and Sun G: MicroRNA-21 induces 5-fluorouracil resistance in human pancreatic cancer cells by regulating PTEN and PDCD4. Cancer Med. 5:693–702. 2016. View Article : Google Scholar : PubMed/NCBI

29 

Song Y, Zuo Y, Qian XL, Chen ZP, Wang SK, Song L and Peng LP: Inhibition of microRNA-21-5p promotes the radiation sensitivity of non-small cell lung cancer through HMSH2. Cell Physiol Biochem. 43:1258–1272. 2017. View Article : Google Scholar : PubMed/NCBI

30 

Cai L, Wang W, Li X, Dong T, Zhang Q, Zhu B, Zhao H and Wu S: MicroRNA-21-5p induces the metastatic phenotype of human cervical carcinoma cells in vitro by targeting the von Hippel-Lindau tumor suppressor. Oncol Lett. 15:5213–5219. 2018.PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Feng Y, Bai F, You Y, Bai F, Wu C, Xin R, Li X and Nie Y: Dysregulated microRNA expression profiles in gastric cancer cells with high peritoneal metastatic potential. Exp Ther Med 16: 4602-4608, 2018.
APA
Feng, Y., Bai, F., You, Y., Bai, F., Wu, C., Xin, R. ... Nie, Y. (2018). Dysregulated microRNA expression profiles in gastric cancer cells with high peritoneal metastatic potential. Experimental and Therapeutic Medicine, 16, 4602-4608. https://doi.org/10.3892/etm.2018.6783
MLA
Feng, Y., Bai, F., You, Y., Bai, F., Wu, C., Xin, R., Li, X., Nie, Y."Dysregulated microRNA expression profiles in gastric cancer cells with high peritoneal metastatic potential". Experimental and Therapeutic Medicine 16.6 (2018): 4602-4608.
Chicago
Feng, Y., Bai, F., You, Y., Bai, F., Wu, C., Xin, R., Li, X., Nie, Y."Dysregulated microRNA expression profiles in gastric cancer cells with high peritoneal metastatic potential". Experimental and Therapeutic Medicine 16, no. 6 (2018): 4602-4608. https://doi.org/10.3892/etm.2018.6783
Copy and paste a formatted citation
x
Spandidos Publications style
Feng Y, Bai F, You Y, Bai F, Wu C, Xin R, Li X and Nie Y: Dysregulated microRNA expression profiles in gastric cancer cells with high peritoneal metastatic potential. Exp Ther Med 16: 4602-4608, 2018.
APA
Feng, Y., Bai, F., You, Y., Bai, F., Wu, C., Xin, R. ... Nie, Y. (2018). Dysregulated microRNA expression profiles in gastric cancer cells with high peritoneal metastatic potential. Experimental and Therapeutic Medicine, 16, 4602-4608. https://doi.org/10.3892/etm.2018.6783
MLA
Feng, Y., Bai, F., You, Y., Bai, F., Wu, C., Xin, R., Li, X., Nie, Y."Dysregulated microRNA expression profiles in gastric cancer cells with high peritoneal metastatic potential". Experimental and Therapeutic Medicine 16.6 (2018): 4602-4608.
Chicago
Feng, Y., Bai, F., You, Y., Bai, F., Wu, C., Xin, R., Li, X., Nie, Y."Dysregulated microRNA expression profiles in gastric cancer cells with high peritoneal metastatic potential". Experimental and Therapeutic Medicine 16, no. 6 (2018): 4602-4608. https://doi.org/10.3892/etm.2018.6783
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