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Article

MicroRNA‑373 promotes cell migration via targeting salt‑inducible kinase 1 expression in melanoma

  • Authors:
    • Xinping Bai
    • Ming Yang
    • Yi Xu
  • View Affiliations / Copyright

    Affiliations: Department of Plastic Surgery, Central Hospital of Wuhan, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430014, P.R. China, Department of Plastic Surgery, The Third People's Hospital of Hubei Province, Wuhan, Hubei 430033, P.R. China
  • Pages: 4759-4764
    |
    Published online on: September 21, 2018
       https://doi.org/10.3892/etm.2018.6784
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Abstract

It is well established that altered expression of microRNAs (miRs) is critical in numerous human cancer types. Nevertheless, the molecular mechanisms of many miRs are yet to be elucidated. In the present study, reverse transcription‑quantitative polymerase chain reaction and western blot analyses, and cell migration assays were performed to verify dysregulation of miR‑373 in melanoma and its biological function. The transcriptional level of miR‑373 was identified to be upregulated in melanoma tissues and cell lines compared with nevus and normal melanocytes. miR‑373 was identified to function as an oncomiR, promoting melanoma cell migration. Notably, miR‑373 was observed to suppress its downstream gene salt‑inducible kinase 1 (SIK1) through directly binding the 3'‑untranslated region of SIK1 expression. Furthermore, reduced SIK1 expression was identified to be responsible for the oncogenic effect of miR‑373. In conclusion, the present study indicates that miR‑373 functions as an oncomiR to promote melanoma progression through targeting SIK1 expression. This may provide a new therapeutic approach for melanoma.
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Copy and paste a formatted citation
Spandidos Publications style
Bai X, Yang M and Xu Y: MicroRNA‑373 promotes cell migration via targeting salt‑inducible kinase 1 expression in melanoma. Exp Ther Med 16: 4759-4764, 2018.
APA
Bai, X., Yang, M., & Xu, Y. (2018). MicroRNA‑373 promotes cell migration via targeting salt‑inducible kinase 1 expression in melanoma. Experimental and Therapeutic Medicine, 16, 4759-4764. https://doi.org/10.3892/etm.2018.6784
MLA
Bai, X., Yang, M., Xu, Y."MicroRNA‑373 promotes cell migration via targeting salt‑inducible kinase 1 expression in melanoma". Experimental and Therapeutic Medicine 16.6 (2018): 4759-4764.
Chicago
Bai, X., Yang, M., Xu, Y."MicroRNA‑373 promotes cell migration via targeting salt‑inducible kinase 1 expression in melanoma". Experimental and Therapeutic Medicine 16, no. 6 (2018): 4759-4764. https://doi.org/10.3892/etm.2018.6784
Copy and paste a formatted citation
x
Spandidos Publications style
Bai X, Yang M and Xu Y: MicroRNA‑373 promotes cell migration via targeting salt‑inducible kinase 1 expression in melanoma. Exp Ther Med 16: 4759-4764, 2018.
APA
Bai, X., Yang, M., & Xu, Y. (2018). MicroRNA‑373 promotes cell migration via targeting salt‑inducible kinase 1 expression in melanoma. Experimental and Therapeutic Medicine, 16, 4759-4764. https://doi.org/10.3892/etm.2018.6784
MLA
Bai, X., Yang, M., Xu, Y."MicroRNA‑373 promotes cell migration via targeting salt‑inducible kinase 1 expression in melanoma". Experimental and Therapeutic Medicine 16.6 (2018): 4759-4764.
Chicago
Bai, X., Yang, M., Xu, Y."MicroRNA‑373 promotes cell migration via targeting salt‑inducible kinase 1 expression in melanoma". Experimental and Therapeutic Medicine 16, no. 6 (2018): 4759-4764. https://doi.org/10.3892/etm.2018.6784
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