Long non‑coding RNA LINC01410 promotes colon cancer cell proliferation and invasion by inhibiting miR‑3128

Luo,Jihui; Guo,Yi; Liu,Xiaofei; Yang,Xihua; Xiao,Fangtao; Zhou,Meihua

doi:10.3892/etm.2018.6806

Long non‑coding RNA LINC01410 promotes colon cancer cell proliferation and invasion by inhibiting miR‑3128

Authors:
- Jihui Luo
- Yi Guo
- Xiaofei Liu
- Xihua Yang
- Fangtao Xiao
- Meihua Zhou
View Affiliations

Affiliations: Department of Surgical Oncology, Chenzhou No. 1 People's Hospital, Chenzhou, Hunan 423000, P.R. China, Department of Nursing, Chenzhou No. 1 People's Hospital, Chenzhou, Hunan 423000, P.R. China
Published online on: September 28, 2018 https://doi.org/10.3892/etm.2018.6806
Pages: 4824-4830

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )

Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )

Cited By (CrossRef): 0 citations Loading Articles...

Abstract

A previous study implied that long intergenic non‑coding RNA 1410 (LINC01410) promotes angiogenesis and metastasis of gastric cancer. However, the role of LINC01410 in colon cancer (CC) has remained elusive. In the present study, LINC01410 was identified to be highly expressed in CC tissues compared to adjacent normal tissues. It was indicated that high expression of LINC01410 in CC tissues was associated with poor prognosis. Further functional study suggested that LINC01410 knockdown significantly reduced the proliferation and invasive capacity of HT‑29 and SW620 cells, and inhibited the cell cycle. Regarding the mechanism, LINC01410 was indicated to serve as a sponge for microRNA (miR)‑3128, as evidenced by a luciferase reporter assay. Furthermore, knockdown of LINC01410 significantly increased the levels of miR‑3128. In addition, miR‑3128 was markedly downregulated in CC tissues compared with that in adjacent normal tissues. A rescue assay revealed that inhibition of miR‑3128 significantly abrogated the effects of LINC01410 knockdown on CC cell proliferation and invasion. In conclusion, the present study demonstrated that LINC01410 functions as an oncogene in CC, at least in part by directly inhibiting miR‑3128.

View Figures

View References

1	Karsa LV, Lignini TA, Patnick J, Lambert R and Sauvaget C: The dimensions of the CRC problem. Best Pract Res Clin Gastroenterol. 24:381–396. 2010. View Article : Google Scholar : PubMed/NCBI
2	Armaghany T, Wilson JD, Chu Q and Mills G: Genetic alterations in colorectal cancer. Gastrointest Cancer Res. 5:19–27. 2012.PubMed/NCBI
3	Shan Z, An N, Qin J, Yang J, Sun H and Yang W: Long non-coding RNA Linc00675 suppresses cell proliferation and metastasis in colorectal cancer via acting on miR-942 and Wnt/beta-catenin signaling. Biomed Pharmacother. 101:769–776. 2018. View Article : Google Scholar : PubMed/NCBI
4	Vatandoust S, Price TJ and Karapetis CS: Colorectal cancer: Metastases to a single organ. World J Gastroenterol. 21:11767–11776. 2015. View Article : Google Scholar : PubMed/NCBI
5	Tsai KW, Lo YH, Liu H, Yeh CY, Chen YZ, Hsu CW, Chen WS and Wang JH: Linc00659, a long noncoding RNA, acts as novel oncogene in regulating cancer cell growth in colorectal cancer. Mol Cancer. 17:722018. View Article : Google Scholar : PubMed/NCBI
6	Liu B, Ye B, Yang L, Zhu X, Huang G, Zhu P, Du Y, Wu J, Qin X, Chen R, et al: Long noncoding RNA lncKdm2b is required for ILC3 maintenance by initiation of Zfp292 expression. Nat Immunol. 18:499–508. 2017. View Article : Google Scholar : PubMed/NCBI
7	Ye B, Liu B, Yang L, Zhu X, Zhang D, Wu W, Zhu P, Wang Y, Wang S, Xia P, et al: LncKdm2b controls self-renewal of embryonic stem cells via activating expression of transcription factor Zbtb3. EMBO J. 37:pii: e971742018. View Article : Google Scholar
8	Wu X, He X, Li S, Xu X, Chen X and Zhu H: Long Non-coding RNA ucoo2kmd.1 regulates CD44-dependent cell growth by competing for miR-211-3p in colorectal cancer. PLoS One. 11:e01512872016. View Article : Google Scholar : PubMed/NCBI
9	Saus E, Brunet-Vega A, Iraola-Guzman S, Pegueroles C, Gabaldon T and Pericay C: Long Non-coding RNAs As potential novel prognostic biomarkers in colorectal cancer. Front Genet. 7:542016. View Article : Google Scholar : PubMed/NCBI
10	Li Y, Zhao L, Zhang Y, Guan L, Zhang H, Zhou H, Gao T, Miao P and Sun M: Downregulation of the long non-coding RNA XLOC_010588 inhibits the invasion and migration of colorectal cancer. Oncol Rep. 39:1619–1630. 2018.PubMed/NCBI
11	Chen DL, Lu YX, Zhang JX, Wei XL, Wang F, Zeng ZL, Pan ZZ, Yuan YF, Wang FH, Pelicano H, et al: Long non-coding RNA UICLM promotes colorectal cancer liver metastasis by acting as a ceRNA for microRNA-215 to regulate ZEB2 expression. Theranostics. 7:4836–4849. 2017. View Article : Google Scholar : PubMed/NCBI
12	Zhang JX, Chen ZH, Chen DL, Tian XP, Wang CY, Zhou ZW, Gao Y, Xu Y, Chen C, Zheng ZS, et al: LINC01410-miR-532-NCF2-NF-kB feedback loop promotes gastric cancer angiogenesis and metastasis. Oncogene. 37:2660–2675. 2018. View Article : Google Scholar : PubMed/NCBI
13	Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI
14	Li T, Xu C, Cai B, Zhang M, Gao F and Gan J: Expression and clinicopathological significance of the lncRNA HOXA11-AS in colorectal cancer. Oncol Lett. 12:4155–4160. 2016. View Article : Google Scholar : PubMed/NCBI
15	Sun M, Nie FQ, Wang ZX and De W: Involvement of lncRNA dysregulation in gastric cancer. Histol Histopathol. 31:33–39. 2016.PubMed/NCBI
16	Wang Y, Zhou J, Wang Z, Wang P and Li S: Upregulation of SOX2 activated LncRNA PVT1 expression promotes breast cancer cell growth and invasion. Biochem Biophys Res Commun. 493:429–436. 2017. View Article : Google Scholar : PubMed/NCBI
17	Zeng X, Hu Z, Ke X, Tang H, Wu B, Wei X and Liu Z: Long noncoding RNA DLX6-AS1 promotes renal cell carcinoma progression via miR-26a/PTEN axis. Cell Cycle. 16:2212–2219. 2017. View Article : Google Scholar : PubMed/NCBI
18	Lu X, Huang C, He X, Liu X, Ji J, Zhang E, Wang W and Guo R: A novel long Non-coding RNA, SOX21-AS1, indicates a poor prognosis and promotes lung adenocarcinoma proliferation. Cell Physiol Biochem. 42:1857–1869. 2017. View Article : Google Scholar : PubMed/NCBI
19	Xiong Y, Wang L, Li Y, Chen M, He W and Qi L: The long Non-coding RNA XIST interacted with MiR-124 to modulate bladder cancer growth, invasion and migration by targeting androgen receptor (AR). Cell Physiol Biochem. 43:405–418. 2017. View Article : Google Scholar : PubMed/NCBI
20	Qi H, Wen B, Wu Q, Cheng W, Lou J, Wei J, Huang J, Yao X and Weng G: Long noncoding RNA SNHG7 accelerates prostate cancer proliferation and cycle progression through cyclin D1 by sponging miR-503. Biomed Pharmacother. 102:326–332. 2018. View Article : Google Scholar : PubMed/NCBI
21	Xu R, Zhu X, Chen F, Huang C, Ai K, Wu H, Zhang L and Zhao X: LncRNA XIST/miR-200c regulates the stemness properties and tumourigenicity of human bladder cancer stem cell-like cells. Cancer Cell Int. 18:412018. View Article : Google Scholar : PubMed/NCBI
22	Lu Q, Zhao N, Zha G, Wang H, Tong Q and Xin S: LncRNA HOXA11-AS exerts oncogenic functions by repressing p21 and miR-124 in uveal melanoma. DNA Cell Biol. 36:837–844. 2017. View Article : Google Scholar : PubMed/NCBI
23	Liu Y, Tao Z, Qu J, Zhou X and Zhang C: Long non-coding RNA PCAT7 regulates ELF2 signaling through inhibition of miR-134-5p in nasopharyngeal carcinoma. Biochem Biophys Res Commun. 491:374–381. 2017. View Article : Google Scholar : PubMed/NCBI