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Article

miR‑671‑5p inhibits gastric cancer cell proliferation and promotes cell apoptosis by targeting URGCP

  • Authors:
    • Tiefeng Qiu
    • Keping Wang
    • Xianwen Li
    • Jianhua Jin
  • View Affiliations / Copyright

    Affiliations: Department of Respiratory Medicine, Wujin Hospital Affiliated to Jiangsu University, Changzhou, Jiangsu 213002, P.R. China, Department of Thoracic Surgery, Chest Hospital of Nanjing, Nanjing, Jiangsu 210029, P.R. China, Department of Medical Oncology, Wujin Hospital Affiliated to Jiangsu University, Changzhou, Jiangsu 213002, P.R. China
  • Pages: 4753-4758
    |
    Published online on: October 1, 2018
       https://doi.org/10.3892/etm.2018.6813
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Abstract

Various studies have demonstrated that microRNA (miRNA) serves an important role in the development of gastric cancer. However, the expression level, clinical significance and the biological function of miRNA in gastric cancer remain largely unknown. The present study investigated the exact roles of miR‑671‑5p in gastric cancer, confirmed its target and explored its mechanism. Initially, the low expression levels of miR‑671‑5p in gastric cancer cells were confirmed by reverse transcription‑quantitative polymerase chain reaction. TargetScan and MiRanda databases were utilized to forecast the target genes of miR‑671‑5p, and the prediction was verified by dual‑luciferase reporter assay and western blot analysis. Cell Counting Kit‑8 was used for cell proliferation detection. An annexin V‑fluorescein isothiocyanate kit was used for cell apoptosis determination. Western blot analysis was adopted to measure the protein expression levels in different groups. The results of the present study revealed that there were lower expression levels of miR‑671‑5p in gastric cancer cells than in normal gastric cells. Upregulator of cell proliferation (URGCP) is a direct target of miR‑671‑5p and it may be negatively regulated by miR‑671‑5p. miR‑671‑5p mimics induced reduction of MKN28 cell proliferation. miR‑671‑5p mimics caused upregulation of MKN28 cell apoptosis. In addition, western blotting results indicated that the ratio of B‑cell lymphoma 2 (Bcl‑2)/Bcl‑2‑associated X protein was significantly decreased in the miR‑671‑5p mimic group compared with the negative control group (P<0.01). These results suggested that miR‑671‑5p had a protective role in gastric cancer through inhibiting gastric cancer cell proliferation and promoting cell apoptosis by targeting URGCP. Therefore, miR‑671‑5p may be an effective therapeutic target for gastric cancer.
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Copy and paste a formatted citation
Spandidos Publications style
Qiu T, Wang K, Li X and Jin J: miR‑671‑5p inhibits gastric cancer cell proliferation and promotes cell apoptosis by targeting URGCP. Exp Ther Med 16: 4753-4758, 2018.
APA
Qiu, T., Wang, K., Li, X., & Jin, J. (2018). miR‑671‑5p inhibits gastric cancer cell proliferation and promotes cell apoptosis by targeting URGCP. Experimental and Therapeutic Medicine, 16, 4753-4758. https://doi.org/10.3892/etm.2018.6813
MLA
Qiu, T., Wang, K., Li, X., Jin, J."miR‑671‑5p inhibits gastric cancer cell proliferation and promotes cell apoptosis by targeting URGCP". Experimental and Therapeutic Medicine 16.6 (2018): 4753-4758.
Chicago
Qiu, T., Wang, K., Li, X., Jin, J."miR‑671‑5p inhibits gastric cancer cell proliferation and promotes cell apoptosis by targeting URGCP". Experimental and Therapeutic Medicine 16, no. 6 (2018): 4753-4758. https://doi.org/10.3892/etm.2018.6813
Copy and paste a formatted citation
x
Spandidos Publications style
Qiu T, Wang K, Li X and Jin J: miR‑671‑5p inhibits gastric cancer cell proliferation and promotes cell apoptosis by targeting URGCP. Exp Ther Med 16: 4753-4758, 2018.
APA
Qiu, T., Wang, K., Li, X., & Jin, J. (2018). miR‑671‑5p inhibits gastric cancer cell proliferation and promotes cell apoptosis by targeting URGCP. Experimental and Therapeutic Medicine, 16, 4753-4758. https://doi.org/10.3892/etm.2018.6813
MLA
Qiu, T., Wang, K., Li, X., Jin, J."miR‑671‑5p inhibits gastric cancer cell proliferation and promotes cell apoptosis by targeting URGCP". Experimental and Therapeutic Medicine 16.6 (2018): 4753-4758.
Chicago
Qiu, T., Wang, K., Li, X., Jin, J."miR‑671‑5p inhibits gastric cancer cell proliferation and promotes cell apoptosis by targeting URGCP". Experimental and Therapeutic Medicine 16, no. 6 (2018): 4753-4758. https://doi.org/10.3892/etm.2018.6813
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