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PVT1 regulates inflammation and cardiac function via the MAPK/NF‑κB pathway in a sepsis model

  • Authors:
    • Fang Feng
    • Yan Qi
    • Chenming Dong
    • Chaohui Yang
  • View Affiliations / Copyright

    Affiliations: Department of Critical Care Medicine, The Second Hospital of Lanzhou University, Lanzhou, Gansu 730000, P.R. China
    Copyright: © Feng et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 4471-4478
    |
    Published online on: October 1, 2018
       https://doi.org/10.3892/etm.2018.6814
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Abstract

The aim of the present study was to investigate the role of plasmacytoma variant translocation gene 1 (PVT1) in the occurrence and development of sepsis‑induced inflammation and cardiac dysfunction and its underlying mechanism. A sepsis rat model was first established by cecal ligation and puncture. The mRNA levels of PVT1 and microRNA‑143 in the myocardial tissues of rats were detected by reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR) analysis. Cardiac function, levels of myocardial injury markers and inflammatory indicators were detected following PVT1 knockdown. The regulatory effect of microRNA‑143 on PVT1 was assessed using a luciferase reporter gene assay and RT‑qPCR analysis. The specific role of PVT1 in regulating the mitogen‑activated protein kinase (MAPK)/nuclear factor (NF)‑κB pathway was detected using western blot analysis. PVT1 was downregulated and microRNA‑143 was upregulated in the myocardial tissues of sepsis rats. The left ventricular peak pressure was markedly decreased in the sepsis rats. By contrast, the left ventricular end diastolic pressure, levels of inflammatory indicators, myocardial injury markers and complement proteins of C5 and C5a were increased in the sepsis rats. The above changes were reversed by PVT1 knockdown or the upregulation of microRNA‑143. MicroRNA‑143 was confirmed as being bound to PVT1 using the luciferase reporter gene assay and RT‑qPCR analysis. Upregulated PVT1 was capable of activating the MAPK/NF‑κB pathway. Taken together, PVT1 was upregulated in the myocardial tissues of sepsis rats, which inhibited cardiac function and promoted the secretion of inflammatory factors; and the mechanism was associated with the MAPK/NF‑κB pathway.
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1 

Liu V, Escobar GJ, Greene JD, Soule J, Whippy A, Angus DC and Iwashyna TJ: Hospital deaths in patients with sepsis from 2 independent cohorts. JAMA. 312:90–92. 2014. View Article : Google Scholar : PubMed/NCBI

2 

Zanotti-Cavazzoni SL and Hollenberg SM: Cardiac dysfunction in severe sepsis and septic shock. Curr Opin Crit Care. 15:392–397. 2009. View Article : Google Scholar : PubMed/NCBI

3 

Lodha R, Arun S, Vivekanandhan S, Kohli U and Kabra SK: Myocardial cell injury is common in children with septic shock. Acta Paediatr. 98:478–481. 2009. View Article : Google Scholar : PubMed/NCBI

4 

Feng Y, Zou L, Zhang M, Li Y, Chen C and Chao W: MyD88 and Trif signaling play distinct roles in cardiac dysfunction and mortality during endotoxin shock and polymicrobial sepsis. Anesthesiology. 115:555–567. 2011. View Article : Google Scholar : PubMed/NCBI

5 

Duncan DJ, Yang Z, Hopkins PM, Steele DS and Harrison SM: TNF-alpha and IL-1beta increase Ca2+ leak from the sarcoplasmic reticulum and susceptibility to arrhythmia in rat ventricular myocytes. Cell Calcium. 47:378–386. 2010. View Article : Google Scholar : PubMed/NCBI

6 

Quinn JJ and Chang HY: Unique features of long non-coding RNA biogenesis and function. Nat Rev Genet. 17:47–62. 2016. View Article : Google Scholar : PubMed/NCBI

7 

Wu H, Yang L and Chen LL: The diversity of long noncoding RNAs and their generation. Trends Genet. 33:540–552. 2017. View Article : Google Scholar : PubMed/NCBI

8 

Gerstein MB, Bruce C, Rozowsky JS, Zheng D, Du J, Korbel JO, Emanuelsson O, Zhang ZD, Weissman S and Snyder M: What is a gene, post-ENCODE? History and updated definition. Genome Res. 17:669–681. 2007. View Article : Google Scholar : PubMed/NCBI

9 

Hanson RL, Craig DW, Millis MP, Yeatts KA, Kobes S, Pearson JV, Lee AM, Knowler WC, Nelson RG and Wolford JK: Identification of PVT1 as a candidate gene for end-stage renal disease in type 2 diabetes using a pooling-based genome-wide single nucleotide polymorphism association study. Diabetes. 56:975–983. 2007. View Article : Google Scholar : PubMed/NCBI

10 

Birnbaum S, Ludwig KU, Reutter H, Herms S, Steffens M, Rubini M, Baluardo C, Ferrian M, de Assis Almeida N, Alblas MA, et al: Key susceptibility locus for nonsyndromic cleft lip with or without cleft palate on chromosome 8q24. Nat Genet. 41:473–477. 2009. View Article : Google Scholar : PubMed/NCBI

11 

Wang N, Liu X, Zheng X, Cao H, Wei G, Zhu Y, Fan S, Zhou H and Zheng J: Ulinastatin is a novel candidate drug for sepsis and secondary acute lung injury, evidence from an optimized CLP rat model. Int Immunopharmacol. 17:799–807. 2013. View Article : Google Scholar : PubMed/NCBI

12 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI

13 

Vincent JL and Marshall JC: Surviving sepsis: A guide to the guidelines. Crit Care. 12:1622008. View Article : Google Scholar : PubMed/NCBI

14 

Hobai IA, Edgecomb J, LaBarge K and Colucci WS: Dysregulation of intracellular calcium transporters in animal models of sepsis-induced cardiomyopathy. Shock. 43:3–15. 2015. View Article : Google Scholar : PubMed/NCBI

15 

Tessier JP, Thurner B, Jüngling E, Lückhoff A and Fischer Y: Impairment of glucose metabolism in hearts from rats treated with endotoxin. Cardiovasc Res. 60:119–130. 2003. View Article : Google Scholar : PubMed/NCBI

16 

Fallach R, Shainberg A, Avlas O, Fainblut M, Chepurko Y, Porat E and Hochhauser E: Cardiomyocyte Toll-like receptor 4 is involved in heart dysfunction following septic shock or myocardial ischemia. J Mol Cell Cardiol. 48:1236–1244. 2010. View Article : Google Scholar : PubMed/NCBI

17 

Horton JW, Maass D, White J and Sanders B: Nitric oxide modulation of TNF-alpha-induced cardiac contractile dysfunction is concentration dependent. Am J Physiol Heart Circ Physiol. 278:H1955–H1965. 2000. View Article : Google Scholar : PubMed/NCBI

18 

Eichenholz PW, Eichacker PQ, Hoffman WD, Banks SM, Parrillo JE, Danner RL and Natanson C: Tumor necrosis factor challenges in canines: Patterns of cardiovascular dysfunction. Am J Physiol. 263:H668–H675. 1992.PubMed/NCBI

19 

Abraham E, Anzueto A, Gutierrez G, Tessler S, Pedro San G, Wunderink R, Dal Nogare A, Nasraway S, Berman S, Cooney R, et al: Double-blind randomised controlled trial of monoclonal antibody to human tumour necrosis factor in treatment of septic shock. NORASEPT II study group. Lancet. 351:929–933. 1998. View Article : Google Scholar : PubMed/NCBI

20 

Damas P, Ledoux D, Nys M, Vrindts Y, De Groote D, Franchimont P and Lamy M: Cytokine serum level during severe sepsis in human IL-6 as a marker of severity. Ann Surg. 215:356–362. 1992. View Article : Google Scholar : PubMed/NCBI

21 

Niederbichler AD, Hoesel LM, Westfall MV, Gao H, Ipaktchi KR, Sun L, Zetoune FS, Su GL, Arbabi S, Sarma JV, et al: An essential role for complement C5a in the pathogenesis of septic cardiac dysfunction. J Exp Med. 203:53–61. 2006. View Article : Google Scholar : PubMed/NCBI

22 

Schulz R, Nava E and Moncada S: Induction and potential biological relevance of a Ca(2+)-independent nitric oxide synthase in the myocardium. Br J Pharmacol. 105:575–580. 1992. View Article : Google Scholar : PubMed/NCBI

23 

Kim EK and Choi EJ: Pathological roles of MAPK signaling pathways in human diseases. Biochim Biophys Acta. 1802:396–405. 2010. View Article : Google Scholar : PubMed/NCBI

24 

Ridder DA and Schwaninger M: NF-kappaB signaling in cerebral ischemia. Neuroscience. 158:995–1006. 2009. View Article : Google Scholar : PubMed/NCBI

25 

Zhang F, Li N, Jiang L, Chen L and Huang M: Neuroprotective effects of (−)-Epigallocatechin-3-Gallate against focal cerebral ischemia/reperfusion injury in rats through attenuation of inflammation. Neurochem Res. 40:1691–1698. 2015. View Article : Google Scholar : PubMed/NCBI

26 

Wang X, Hu D, Zhang L, Lian G, Zhao S, Wang C, Yin J, Wu C and Yang J: Gomisin a inhibits lipopolysaccharide-induced inflammatory responses in N9 microglia via blocking the NF-kappaB/MAPKs pathway. Food Chem Toxicol. 63:119–127. 2014. View Article : Google Scholar : PubMed/NCBI

27 

Raza SS, Khan MM, Ahmad A, Ashafaq M and Islam F, Wagner AP, Safhi MM and Islam F: Neuroprotective effect of naringenin is mediated through suppression of NF-kappaB signaling pathway in experimental stroke. Neuroscience. 230:157–171. 2013. View Article : Google Scholar : PubMed/NCBI

28 

Dhawan P and Richmond A: A novel NF-kappa B-inducing kinase-MAPK signaling pathway up-regulates NF-kappa B activity in melanoma cells. J Biol Chem. 277:7920–7928. 2002. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Feng F, Qi Y, Dong C and Yang C: PVT1 regulates inflammation and cardiac function via the MAPK/NF‑κB pathway in a sepsis model. Exp Ther Med 16: 4471-4478, 2018.
APA
Feng, F., Qi, Y., Dong, C., & Yang, C. (2018). PVT1 regulates inflammation and cardiac function via the MAPK/NF‑κB pathway in a sepsis model. Experimental and Therapeutic Medicine, 16, 4471-4478. https://doi.org/10.3892/etm.2018.6814
MLA
Feng, F., Qi, Y., Dong, C., Yang, C."PVT1 regulates inflammation and cardiac function via the MAPK/NF‑κB pathway in a sepsis model". Experimental and Therapeutic Medicine 16.6 (2018): 4471-4478.
Chicago
Feng, F., Qi, Y., Dong, C., Yang, C."PVT1 regulates inflammation and cardiac function via the MAPK/NF‑κB pathway in a sepsis model". Experimental and Therapeutic Medicine 16, no. 6 (2018): 4471-4478. https://doi.org/10.3892/etm.2018.6814
Copy and paste a formatted citation
x
Spandidos Publications style
Feng F, Qi Y, Dong C and Yang C: PVT1 regulates inflammation and cardiac function via the MAPK/NF‑κB pathway in a sepsis model. Exp Ther Med 16: 4471-4478, 2018.
APA
Feng, F., Qi, Y., Dong, C., & Yang, C. (2018). PVT1 regulates inflammation and cardiac function via the MAPK/NF‑κB pathway in a sepsis model. Experimental and Therapeutic Medicine, 16, 4471-4478. https://doi.org/10.3892/etm.2018.6814
MLA
Feng, F., Qi, Y., Dong, C., Yang, C."PVT1 regulates inflammation and cardiac function via the MAPK/NF‑κB pathway in a sepsis model". Experimental and Therapeutic Medicine 16.6 (2018): 4471-4478.
Chicago
Feng, F., Qi, Y., Dong, C., Yang, C."PVT1 regulates inflammation and cardiac function via the MAPK/NF‑κB pathway in a sepsis model". Experimental and Therapeutic Medicine 16, no. 6 (2018): 4471-4478. https://doi.org/10.3892/etm.2018.6814
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