Open Access

Inhibitory effect of microRNA‑455‑5p on biological functions of esophageal squamous cell carcinoma Eca109 cells via Rab31

  • Authors:
    • Ying Liu
    • Yanping Tang
    • Ping Li
  • View Affiliations

  • Published online on: October 2, 2018     https://doi.org/10.3892/etm.2018.6820
  • Pages: 4959-4966
  • Copyright: © Liu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The aim of the present study was to examine microRNA (miRNA or miR)‑455‑5p expression in esophageal squamous cell carcinoma (ESCC) at the tissue and cellular levels in order to elucidate its biological roles. A total of 60 patients with ESCC were enrolled in the present study and reverse transcription‑quantitative polymerase chain reaction was used to measure the expression of miR‑455‑5p. ESCC Eca109 cells were transfected with miR‑NC, miR‑455‑5p mimics or inhibitor and a Cell Counting Kit‑8 assay was used to assess proliferation. To investigate the migration and invasion abilities of Eca109 cells, Transwell and Matrigel assays were performed. Western blotting was employed to measure Rab31 protein expression, while a rescue assay was utilized to study the biological roles of miR‑455‑5p and Rab31 in Eca109 cells. To determine whether Rab31 is a direct target of miR‑455‑5p, a dual luciferase reporter assay was performed. The results revealed that miR‑455‑5p expression was decreased in ESCC tissues and was negatively correlated with metastasis and pathogenesis. In vitro overexpression of miR‑455‑5p inhibited the proliferation, migration and invasion of ESCC Eca109 cells. Furthermore, miR‑455‑5p regulated the expression of Rab31 protein in Eca109 cells. Rab31 overexpression promoted the proliferation, migration and invasion of Eca109 cells. Luciferase reporter assay results revealed that miR‑455‑5p is able to bind with the 3'‑untranslated region of Rab31 mRNA to regulate its expression. In summary, the results of the present study suggest that miR‑455‑5p expression is decreased in ESCC tissues and is miR‑455‑5p is negatively correlated with lymphatic metastasis and differentiation. As a tumor‑suppressor gene, miR‑455‑5p inhibits the proliferation, migration and invasion of ESCC Eca109 cells by suppressing the expression of Rab31.
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December-2018
Volume 16 Issue 6

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Liu Y, Tang Y and Li P: Inhibitory effect of microRNA‑455‑5p on biological functions of esophageal squamous cell carcinoma Eca109 cells via Rab31. Exp Ther Med 16: 4959-4966, 2018
APA
Liu, Y., Tang, Y., & Li, P. (2018). Inhibitory effect of microRNA‑455‑5p on biological functions of esophageal squamous cell carcinoma Eca109 cells via Rab31. Experimental and Therapeutic Medicine, 16, 4959-4966. https://doi.org/10.3892/etm.2018.6820
MLA
Liu, Y., Tang, Y., Li, P."Inhibitory effect of microRNA‑455‑5p on biological functions of esophageal squamous cell carcinoma Eca109 cells via Rab31". Experimental and Therapeutic Medicine 16.6 (2018): 4959-4966.
Chicago
Liu, Y., Tang, Y., Li, P."Inhibitory effect of microRNA‑455‑5p on biological functions of esophageal squamous cell carcinoma Eca109 cells via Rab31". Experimental and Therapeutic Medicine 16, no. 6 (2018): 4959-4966. https://doi.org/10.3892/etm.2018.6820