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Epimedium protects steroid‑induced avascular necrosis of femoral head in rats by inhibiting autophagy

  • Authors:
    • Su Liu
    • Yunzong Huang
    • Chuangli Wang
    • Shoujing Tian
    • Youjia Xu
    • Jianfei Ge
  • View Affiliations / Copyright

    Affiliations: Department of Orthopedics, Zhangjiagang Hospital Affiliated to Soochow University, Zhangjiagang, Jiangsu 215600, P.R. China
    Copyright: © Liu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 5047-5052
    |
    Published online on: October 3, 2018
       https://doi.org/10.3892/etm.2018.6827
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Abstract

The effect of epimedium extracting solution on bone mineral density (BMD) of steroid‑induced avascular necrosis of femoral head (SANFH) in rats was evaluated to further explore its function mechanism. Twenty-four Sprague‑Dawley (SD) rats (male/female: 1/1) were randomly divided into three groups: the control (n=8), the glucocorticoid (n=8) and the epimedium (n=8) group. Rats in the glucocorticoid and the epimedium group were injected with prednisolone acetate injection in gluteal muscles with 15 mg/kg/day twice a week. The epimedium group was given 10 ml/kg ephedra extracting solution containing crude drug with the concentration of 1.5 g/ml daily by gavage. After 6 weeks, all the experimental rats were sacrificed and materials were extracted. The expression of autophagy‑related proteins were detected by observing the bone of the femoral head. After comparison of the control group with the model group in BMD, it was found that there were significant differences (P<0.05). There were no significant differences between the control and the epimedium group (P>0.05). Neither between the glucocorticoid and the epimedium group (P<0.05). Epimedium extracting solution can significantly enhance the BMD of femoral heads, prevent osteoporosis and lead to collapse, increase the expression of apoptotic and protective proteins and reduce the expression of autophagy‑related proteins, thus providing a preliminary theoretical study for the prevention and treatment of SANFH.
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Copy and paste a formatted citation
Spandidos Publications style
Liu S, Huang Y, Wang C, Tian S, Xu Y and Ge J: Epimedium protects steroid‑induced avascular necrosis of femoral head in rats by inhibiting autophagy. Exp Ther Med 16: 5047-5052, 2018.
APA
Liu, S., Huang, Y., Wang, C., Tian, S., Xu, Y., & Ge, J. (2018). Epimedium protects steroid‑induced avascular necrosis of femoral head in rats by inhibiting autophagy. Experimental and Therapeutic Medicine, 16, 5047-5052. https://doi.org/10.3892/etm.2018.6827
MLA
Liu, S., Huang, Y., Wang, C., Tian, S., Xu, Y., Ge, J."Epimedium protects steroid‑induced avascular necrosis of femoral head in rats by inhibiting autophagy". Experimental and Therapeutic Medicine 16.6 (2018): 5047-5052.
Chicago
Liu, S., Huang, Y., Wang, C., Tian, S., Xu, Y., Ge, J."Epimedium protects steroid‑induced avascular necrosis of femoral head in rats by inhibiting autophagy". Experimental and Therapeutic Medicine 16, no. 6 (2018): 5047-5052. https://doi.org/10.3892/etm.2018.6827
Copy and paste a formatted citation
x
Spandidos Publications style
Liu S, Huang Y, Wang C, Tian S, Xu Y and Ge J: Epimedium protects steroid‑induced avascular necrosis of femoral head in rats by inhibiting autophagy. Exp Ther Med 16: 5047-5052, 2018.
APA
Liu, S., Huang, Y., Wang, C., Tian, S., Xu, Y., & Ge, J. (2018). Epimedium protects steroid‑induced avascular necrosis of femoral head in rats by inhibiting autophagy. Experimental and Therapeutic Medicine, 16, 5047-5052. https://doi.org/10.3892/etm.2018.6827
MLA
Liu, S., Huang, Y., Wang, C., Tian, S., Xu, Y., Ge, J."Epimedium protects steroid‑induced avascular necrosis of femoral head in rats by inhibiting autophagy". Experimental and Therapeutic Medicine 16.6 (2018): 5047-5052.
Chicago
Liu, S., Huang, Y., Wang, C., Tian, S., Xu, Y., Ge, J."Epimedium protects steroid‑induced avascular necrosis of femoral head in rats by inhibiting autophagy". Experimental and Therapeutic Medicine 16, no. 6 (2018): 5047-5052. https://doi.org/10.3892/etm.2018.6827
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