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Simultaneous isolation of mesenchymal stem cells and endothelial progenitor cells derived from murine bone marrow

  • Authors:
    • Xiaoyi Wang
    • Zongsheng Zhao
    • Hongwei Zhang
    • Jixue Hou
    • Wenlei Feng
    • Meng Zhang
    • Jun Guo
    • Jie Xia
    • Quanhu Ge
    • Xueling Chen
    • Xiangwei Wu
  • View Affiliations / Copyright

    Affiliations: Laboratory of Translational Medicine, School of Medicine, Shihezi University, Shihezi, Xinjiang 832008, P.R. China, Department of Animal Genetic Breeding and Reproduction, College of Animal Science and Technology, Shihezi University, Shihezi, Xinjiang 832008, P.R. China, Department of General Surgery, First Affiliated Hospital, Shihezi University, Shihezi, Xinjiang 832008, P.R. China, Department of Immunology, School of Medicine, Shihezi University, Shihezi, Xinjiang 832008, P.R. China
    Copyright: © Wang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 5171-5177
    |
    Published online on: October 11, 2018
       https://doi.org/10.3892/etm.2018.6844
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Abstract

Mesenchymal stem or stromal cells (MSCs) are identified as sources of pluripotent stem cells with varying degrees of plasticity. Endothelial progenitor cells (EPCs) originate from either bone marrow (BM) or peripheral blood and can mature into cells that line the lumen of blood vessels. MSC and EPC therapies exhibit promising results in a variety of diseases. The current study described the simultaneous isolation of EPCs and MSCs from murine BM using a straightforward approach. The method is based on differences in attachment time and trypsin sensitivity of MSCs and EPCs. The proposed method revealed characteristics of isolated cells. Isolated MSCs were positive for cell surface markers, cluster of differentiation (CD)29, CD44 and stem cell antigen‑1 (Sca‑1), and negative for hematopoietic surface markers, CD45 and CD11b. Isolated EPCs were positive for Sca‑1 and vascular endothelial growth factor receptor 2 and CD133. The results indicate that the proposed method ensured simultaneous isolation of homogenous populations of MSCs and EPCs from murine BM.
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Copy and paste a formatted citation
Spandidos Publications style
Wang X, Zhao Z, Zhang H, Hou J, Feng W, Zhang M, Guo J, Xia J, Ge Q, Chen X, Chen X, et al: Simultaneous isolation of mesenchymal stem cells and endothelial progenitor cells derived from murine bone marrow. Exp Ther Med 16: 5171-5177, 2018.
APA
Wang, X., Zhao, Z., Zhang, H., Hou, J., Feng, W., Zhang, M. ... Wu, X. (2018). Simultaneous isolation of mesenchymal stem cells and endothelial progenitor cells derived from murine bone marrow. Experimental and Therapeutic Medicine, 16, 5171-5177. https://doi.org/10.3892/etm.2018.6844
MLA
Wang, X., Zhao, Z., Zhang, H., Hou, J., Feng, W., Zhang, M., Guo, J., Xia, J., Ge, Q., Chen, X., Wu, X."Simultaneous isolation of mesenchymal stem cells and endothelial progenitor cells derived from murine bone marrow". Experimental and Therapeutic Medicine 16.6 (2018): 5171-5177.
Chicago
Wang, X., Zhao, Z., Zhang, H., Hou, J., Feng, W., Zhang, M., Guo, J., Xia, J., Ge, Q., Chen, X., Wu, X."Simultaneous isolation of mesenchymal stem cells and endothelial progenitor cells derived from murine bone marrow". Experimental and Therapeutic Medicine 16, no. 6 (2018): 5171-5177. https://doi.org/10.3892/etm.2018.6844
Copy and paste a formatted citation
x
Spandidos Publications style
Wang X, Zhao Z, Zhang H, Hou J, Feng W, Zhang M, Guo J, Xia J, Ge Q, Chen X, Chen X, et al: Simultaneous isolation of mesenchymal stem cells and endothelial progenitor cells derived from murine bone marrow. Exp Ther Med 16: 5171-5177, 2018.
APA
Wang, X., Zhao, Z., Zhang, H., Hou, J., Feng, W., Zhang, M. ... Wu, X. (2018). Simultaneous isolation of mesenchymal stem cells and endothelial progenitor cells derived from murine bone marrow. Experimental and Therapeutic Medicine, 16, 5171-5177. https://doi.org/10.3892/etm.2018.6844
MLA
Wang, X., Zhao, Z., Zhang, H., Hou, J., Feng, W., Zhang, M., Guo, J., Xia, J., Ge, Q., Chen, X., Wu, X."Simultaneous isolation of mesenchymal stem cells and endothelial progenitor cells derived from murine bone marrow". Experimental and Therapeutic Medicine 16.6 (2018): 5171-5177.
Chicago
Wang, X., Zhao, Z., Zhang, H., Hou, J., Feng, W., Zhang, M., Guo, J., Xia, J., Ge, Q., Chen, X., Wu, X."Simultaneous isolation of mesenchymal stem cells and endothelial progenitor cells derived from murine bone marrow". Experimental and Therapeutic Medicine 16, no. 6 (2018): 5171-5177. https://doi.org/10.3892/etm.2018.6844
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