Effects of EDC-PEI heparinization on allogeneic vascular antigens and inflammation levels via a rat abdominal aorta transplantation model

Han,Feng; Tian,Puxun; Xia,Xinxin; Wang,Yuxiang; Dou,Meng; Zheng,Jin; Ding,Xiaoming; Xue,Wujun; Ding,Chenguang

doi:10.3892/etm.2018.6879

Effects of EDC-PEI heparinization on allogeneic vascular antigens and inflammation levels via a rat abdominal aorta transplantation model

Authors:
- Feng Han
- Puxun Tian
- Xinxin Xia
- Yuxiang Wang
- Meng Dou
- Jin Zheng
- Xiaoming Ding
- Wujun Xue
- Chenguang Ding
View Affiliations

Affiliations: Department of Kidney Transplantation, Nephropathy Hospital, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi 710061, P.R. China, Department of Traditional Chinese Medicine, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi 710061, P.R. China
Published online on: October 19, 2018 https://doi.org/10.3892/etm.2018.6879
Pages: 5053-5058

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )

Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )

Cited By (CrossRef): 0 citations Loading Articles...

Abstract

The objective of this study was to investigate the possibility of increasing the remote patency rate of allogeneic vessel transplantation through studying the effects of N-ethyl-N-(3-dimethylaminopropyl)-carbodiimide and polyethyleneimine (EDC-PEI) heparinization on allogeneic vascular antigens and inflammation levels. Forty rats were randomly divided into the control group, the EDC group, the PEI group and the EDC-PEI group. The rat abdominal aorta was used as the object of study, and the transplanted blood vessels were pretreated with EDC as the water-soluble cross-linking agent and PEI as the heparin-coated carrier. A rat abdominal aorta allogeneic transplantation model was established. Ultrasonic examination was used for observation of patency of proximal and distal anastomosis in each group. The tissue repair after abdominal aorta transplantation in each group was examined by H&E staining. The biomechanics, denaturation temperature and blasting strength of each group were compared. The levels of IL-1, IL-6 and TNF-α in serum of rats were measured by Elisa method, and the expression of MHC-II and α-GAL antigens in blood vessels were detected by immunohistochemistry. There were different degrees of thickening and inflammatory cell aggregation in the abdominal aorta of rats in the control, EDC and PEI groups, but there was no obvious lesion in the EDC-PEI group. Compared with the four groups, the mechanical characteristics of the EDC group decreased significantly, and the stenosis rate of anastomotic stoma in the EDC group was higher than that in the EDC-PEI and PEI groups (P<0.05). The denaturation temperature of the PEI group was lower than that of the EDC and EDC-PEI groups (P<0.05). The mechanical property and vascular bursting strength in the EDC-PEI group were similar to those in the control group. At the same time, it has more significant advantages than the other three groups in removing the vascular antigens MHC-II and α-GAL and reducing the level of inflammatory reaction, thus increasing the remote patency rate of allogeneic vascular transplantation. The inflammatory response and vascular antigenicity after transplantation are effectively reduced via the rat abdominal aorta transplantation model treated with allogeneic EDC-PEI heparinization, which has a higher remote patency rate.

View Figures

View References

1	Ohmes LB, Di Franco A, Di Giammarco G, Rosati CM, Lau C, Girardi LN, Massetti M and Gaudino M: Techniques for intraoperative graft assessment in coronary artery bypass surgery. J Thorac Dis. 9 Suppl 4:S327–S332. 2017. View Article : Google Scholar : PubMed/NCBI
2	Raja SG: Two decades of off-pump coronary artery bypass surgery: Harefield experience. J Thorac Dis. 8 Suppl 10:S824–S828. 2016. View Article : Google Scholar : PubMed/NCBI
3	He GW and Taggart DP: Antispastic management in arterial grafts in coronary artery bypass grafting surgery. Ann Thorac Surg. 102:659–668. 2016. View Article : Google Scholar : PubMed/NCBI
4	Ward AO, Caputo M, Angelini GD, George SJ and Zakkar M: Activation and inflammation of the venous endothelium in vein graft disease. Atherosclerosis. 265:266–274. 2017. View Article : Google Scholar : PubMed/NCBI
5	Meng F, Ma J, Wang W and Lin B: Meta-analysis of interleukin 6, 8, and 10 between off-pump and on-pump coronary artery bypass groups. Bosn J Basic Med Sci. 17:85–94. 2017.PubMed/NCBI
6	Lee W, Park KD, Han KD, Suh H, Park JC and Kim YH: Heparinized bovine pericardium as a novel cardiovascular bioprosthesis. Biomaterials. 21:2323–2330. 2000. View Article : Google Scholar : PubMed/NCBI
7	Nosalski R and Guzik TJ: Perivascular adipose tissue inflammation in vascular disease. Br J Pharmacol. 174:3496–3513. 2017. View Article : Google Scholar : PubMed/NCBI
8	Lénárt N, Brough D and Dénes Á: Inflammasomes link vascular disease with neuroinflammation and brain disorders. J Cereb Blood Flow Metab. 36:1668–1685. 2016. View Article : Google Scholar : PubMed/NCBI
9	Amlie-Lefond C and Shaw D: Vascular disease. Handb Clin Neurol. 136:1159–1171. 2016. View Article : Google Scholar : PubMed/NCBI
10	de Franciscis S, Metzinger L and Serra R: The Discovery of novel genomic, transcriptomic, and proteomic biomarkers in cardiovascular and peripheral vascular disease: The state of the art. BioMed Res Int. 2016:78291742016. View Article : Google Scholar : PubMed/NCBI
11	Lukitsch I, Kehr J, Chaykovska L, Wallukat G, Nieminen-Kelhä M, Batuman V, Dragun D and Gollasch M: Renal ischemia and transplantation predispose to vascular constriction mediated by angiotensin II type 1 receptor-activating antibodies. Transplantation. 94:8–13. 2012. View Article : Google Scholar : PubMed/NCBI
12	Yin H and Pickering JG: Cellular senescence and vascular disease: Novel routes to better understanding and therapy. Can J Cardiol. 32:612–623. 2016. View Article : Google Scholar : PubMed/NCBI
13	Moore TM and Gendler E and Gendler E: Viruses adsorbed on musculoskeletal allografts are inactivated by terminal ethylene oxide disinfection. J Orthop Res. 22:1358–1361. 2004. View Article : Google Scholar : PubMed/NCBI
14	Cheshire PA, Herson MR, Cleland H and Akbarzadeh S: Artificial dermal templates: A comparative study of NovoSorb™ Biodegradable Temporising Matrix (BTM) and Integra(^®) Dermal Regeneration Template (DRT). Burns. 42:1088–1096. 2016. View Article : Google Scholar : PubMed/NCBI
15	Han Y, Choo SJ, Kwon H, Lee JW, Chung CH, Kim H, Kwon TW and Cho YP: Effects of upper-extremity vascular access creation on cardiac events in patients undergoing coronary artery bypass grafting. PLoS One. 12:e01841682017. View Article : Google Scholar : PubMed/NCBI
16	Weldon JE, Xiang L, Zhang J, Beers R, Walker DA, Onda M, Hassan R and Pastan I: A recombinant immunotoxin against the tumor-associated antigen mesothelin reengineered for high activity, low off-target toxicity, and reduced antigenicity. Mol Cancer Ther. 12:48–57. 2013. View Article : Google Scholar : PubMed/NCBI
17	Patris E, Giakoumidakis K, Patris V, Kuduvalli M, Argiriou M, Charitos C, Kalaitzis C and Touloupidis S: Perioperative prostate specific antigen levels among coronary artery bypass grafting patients: Does extracorporeal circulation and body temperature induce prostate specific antigen levels alterations? Urol Ann. 7:58–62. 2015. View Article : Google Scholar : PubMed/NCBI
18	Opfermann P, Derhaschnig U, Felli A, Wenisch J, Santer D, Zuckermann A, Dworschak M, Jilma B and Steinlechner B: A pilot study on reparixin, a CXCR1/2 antagonist, to assess safety and efficacy in attenuating ischaemia-reperfusion injury and inflammation after on-pump coronary artery bypass graft surgery. Clin Exp Immunol. 180:131–142. 2015. View Article : Google Scholar : PubMed/NCBI
19	Müller S, Rihs S, Schneider JM, Paredes BE, Seibold I, Brunner T and Mueller C: Soluble TNF-alpha but not transmembrane TNF-alpha sensitizes T cells for enhanced activation-induced cell death. Eur J Immunol. 39:3171–3180. 2009. View Article : Google Scholar : PubMed/NCBI
20	Guo ZP, Hou HT, Jing R, Song ZG, Liu XC and He GW: Plasma protein profiling in patients undergoing coronary artery bypass grafting surgery and clinical significance. Oncotarget. 8:60528–60538. 2017.PubMed/NCBI
21	Abraham AC, Shah SA and Thomopoulos S: Targeting inflammation in rotator cuff tendon degeneration and repair. Tech Shoulder Elbow Surg. 18:84–90. 2017. View Article : Google Scholar
22	Kimura T, Tse K, McArdle S, Gerhardt T, Miller J, Mikulski Z, Sidney J, Sette A, Wolf D and Ley K: Atheroprotective vaccination with MHC-II-restricted ApoB peptides induces peritoneal IL-10-producing CD4 T cells. Am J Physiol Heart Circ Physiol. 312:H781–H790. 2017. View Article : Google Scholar : PubMed/NCBI
23	Schmid PM, Bouazzaoui A, Schmid K, Birner CM, Schach C, Maier LS, Holler E and Endemann DH: Vascular alterations in a murine model of acute graft-versus-host disease are associated with decreased serum levels of adiponectin and an increased activity and vascular expression of indoleamine 2,3-dioxygenase. Cell Transplant. 25:2051–2062. 2016. View Article : Google Scholar : PubMed/NCBI
24	Mazzaccaro D, De Febis E, Settembrini AM, Tassinari L, Carmo M and Settembrini PG: Long-term results of PTFE trilaminate graft versus venous graft and composite graft for below-the-knee revascularization. J Cardiovasc Surg (Torino). 55:685–691. 2014.PubMed/NCBI
25	Malinska A, Podemska Z, Sujka-Kordowska P, Witkiewicz W, Nowicki M, Perek B and Witt M: Caveolin 2: A facultative marker of unfavourable prognosis in long-term patency rate of internal thoracic artery grafts used in coronary artery bypass grafting. Preliminary report. Interact Cardiovasc Thorac Surg. 24:714–720. 2017.PubMed/NCBI