Open Access

microRNA‑200a functions as a tumor suppressor by targeting FOXA1 in glioma

  • Authors:
    • Xiaofeng Chen
    • Kun Liu
    • Ping Yang
    • Weiping Kuang
    • Hongxing Huang
    • Ewen Tu
    • Bo Li
    • Yong Zhu
    • Bin Zhou
    • Lin Yan
  • View Affiliations

  • Published online on: October 29, 2018     https://doi.org/10.3892/etm.2018.6895
  • Pages: 221-229
  • Copyright: © Chen et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

microRNAs (miRs) serve primary roles in certain human malignancies; however, the detailed regulatory mechanism of miR‑200a in glioma progression is yet to be fully elucidated. The current study aimed to assess the expression of miR‑200a in glioma as well as the regulatory mechanism of miR‑200a in glioma cell proliferation, survival and invasion. RT‑qPCR and western blotting were performed to examine mRNA and protein expression. An MTT assay, an EdU incorporation cell proliferation assay and a transwell assay were utilized to assess cell survival, proliferation and invasion. The results indicated that the miR‑200a levels were significantly reduced in glioma tissues compared with normal brain tissues. Levels were also downregulated in glioma cell lines when compared with those in normal human astrocyte cells. Furthermore, low miR‑200a expression was associated with advanced progression of glioma. The overexpression of miR‑200a inhibited glioma cell proliferation, survival and invasion. Results also identified that FOXA1 was a target gene of miR‑200a in glioma cells and that the increased expression of FOXA1 was negatively correlated to the decreased expression of miR‑200a in glioma tissues. Furthermore, FOXA1 expression was negatively mediated by miR‑200a in glioma cells and the overexpression of FOXA1 eliminated the inhibitory effects of miR‑200a on the survival, proliferation and invasion of glioma cells. In conclusion, the current study demonstrated that miR‑200a functions acts as a tumor suppressor in glioma by directly targeting FOXA1 and may thus be a potential candidate for the treatment of glioma.
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January-2019
Volume 17 Issue 1

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Chen X, Liu K, Yang P, Kuang W, Huang H, Tu E, Li B, Zhu Y, Zhou B, Yan L, Yan L, et al: microRNA‑200a functions as a tumor suppressor by targeting FOXA1 in glioma. Exp Ther Med 17: 221-229, 2019
APA
Chen, X., Liu, K., Yang, P., Kuang, W., Huang, H., Tu, E. ... Yan, L. (2019). microRNA‑200a functions as a tumor suppressor by targeting FOXA1 in glioma. Experimental and Therapeutic Medicine, 17, 221-229. https://doi.org/10.3892/etm.2018.6895
MLA
Chen, X., Liu, K., Yang, P., Kuang, W., Huang, H., Tu, E., Li, B., Zhu, Y., Zhou, B., Yan, L."microRNA‑200a functions as a tumor suppressor by targeting FOXA1 in glioma". Experimental and Therapeutic Medicine 17.1 (2019): 221-229.
Chicago
Chen, X., Liu, K., Yang, P., Kuang, W., Huang, H., Tu, E., Li, B., Zhu, Y., Zhou, B., Yan, L."microRNA‑200a functions as a tumor suppressor by targeting FOXA1 in glioma". Experimental and Therapeutic Medicine 17, no. 1 (2019): 221-229. https://doi.org/10.3892/etm.2018.6895