Biological effects of autophagy in mice with sepsis‑induced acute kidney injury

Wu,Yu; Wang,Ling; Meng,Lei; Cao,Guang‑Ke; Zhao,Yu‑Liang; Zhang,Yang

doi:10.3892/etm.2018.6899

Biological effects of autophagy in mice with sepsis‑induced acute kidney injury

Authors:
- Yu Wu
- Ling Wang
- Lei Meng
- Guang‑Ke Cao
- Yu‑Liang Zhao
- Yang Zhang
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Affiliations: Department of Nephrology, Xuzhou Municipal Hospital Affiliated to Xuzhou Medical University, Xuzhou, Jiangsu 221000, P.R. China, Department of Intensive Care Unit, The Affiliated Hospital of Xuzhou Medical University, Xuzhou, Jiangsu 221000, P.R. China, Department of Anesthesiology, Xuzhou Medical University, Xuzhou, Jiangsu 221004, P.R. China
Published online on: October 30, 2018 https://doi.org/10.3892/etm.2018.6899
Pages: 316-322

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Abstract

This study investigated whether autophagy is activated after sepsis‑induced acute kidney injury (AKI) and explored its biological role. Seventy‑two normal C57 mice were randomly divided into sham operation group, cecal ligation and puncture (CLP) group and CLP+3‑MA (autophagy inhibitor) group; 24 mice in each group. Mice in CLP and CLP+3‑MA group were treated with cecal ligation to establish sepsis, while mice in sham operation group were treated with the same surgical operations, but not cecal ligation. Blood samples were collected from 12 mice of each group and the levels of serum creatinine (Cr) and blood urea nitrogen (BUN) were measured. The pathological changes were observed. The remaining 12 mice in each group were kept and the survival rate was recorded. Changes in the expressions of autophagy‑related proteins were detected by reverse transcription‑semi‑quantitative PCR and western blotting. The results revealed that the levels of Cr and BUN in CLP and CLP+3‑MA group were significantly higher than those in sham operation group (P<0.05), and the levels of Cr and BUN in CLP+3‑MA group were higher than those in CLP group (P<0.05). The pathological score of renal injury in CLP+3‑MA group was significantly higher than that of CLP group (P<0.01). The expression levels of Beclin1 and LC3‑Ⅱ/I were significantly increased in CLP group compared to sham operation group (P<0.01), while the expression of p62 was decreased (P<0.01). After 3‑MA treatment the expression levels of Beclin1 and LC3‑II/I were decreased, compared with CLP group, but accumulation of p62 occurred, and the degree of renal injury was increased. In conclusion, AKI induced by sepsis in mice can induce apoptosis and activate autophagy. The activation of autophagy aggravates the renal injury in mice, which in turn inhibits AKI after sepsis.

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