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miR-138 expression in oral herpes simplex and its effect on ICP0

  • Authors:
    • Xiao Yan
    • Jing Sun
    • Kuifeng Yuan
  • View Affiliations / Copyright

    Affiliations: Department of Stomatology, The Second Qilu Hospital of Shandong University, Jinan, Shandong 250033, P.R. China, Department of Pharmacy, The Second Qilu Hospital of Shandong University, Jinan, Shandong 250033, P.R. China
    Copyright: © Yan et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 388-392
    |
    Published online on: November 1, 2018
       https://doi.org/10.3892/etm.2018.6912
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Abstract

This study aimed to investigate the micro ribonucleic acid-138 (miR-138) expression in oral herpes simplex (HS) and its effect on the expression of herpes simplex virus type 1 (HSV-1) lytic gene trans-acting factor infected cell protein 0 (ICP0). Forty-five rat models with oral HS were successfully established (the observation group) and another 40 healthy rats were selected as the control group. The miR-138 expression in serum of rats in the two groups were detected by reverse transcription-quantitative polymerase chain reaction (RT-qPCR). 293T cells infected by HSV-1 were divided into Group A and Group B after 10 days of culture. Group A was transfected by miR-138 mimics and Group B was transfected by miR-138 complementary oligonucleotide inhibitor. The expression levels of miR-138 and ICP0 messenger RNA (mRNA) in the cells of the two groups were detected by RT-PCR, and the expression levels of ICP0 protein were detected by enzyme-linked immunosorbent assay (ELISA). A total of 85 rat models with oral HS were established in this study, but only 45 models were established successfully with a success rate of 56.25%. The expression level of miR-138 in the rat serum in the observation group was higher than that in the control group (P<0.05). In addition, the expression level of ICP0 mRNA in Group A was lower than that in Group B (P<0.05). Moreover, the expression level of ICP0 protein in Group A was lower than that in Group B (P<0.05). Finally, the expression level of miR-138 in HSV was increased, suggesting that the expression of miR-138 may inhibit the expression of ICP0, thus preventing the duplication of HSV-1. Therefore, the expression of miR-138 may be used as a potential therapeutic target for HSV.
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Copy and paste a formatted citation
Spandidos Publications style
Yan X, Sun J and Yuan K: miR-138 expression in oral herpes simplex and its effect on ICP0. Exp Ther Med 17: 388-392, 2019.
APA
Yan, X., Sun, J., & Yuan, K. (2019). miR-138 expression in oral herpes simplex and its effect on ICP0. Experimental and Therapeutic Medicine, 17, 388-392. https://doi.org/10.3892/etm.2018.6912
MLA
Yan, X., Sun, J., Yuan, K."miR-138 expression in oral herpes simplex and its effect on ICP0". Experimental and Therapeutic Medicine 17.1 (2019): 388-392.
Chicago
Yan, X., Sun, J., Yuan, K."miR-138 expression in oral herpes simplex and its effect on ICP0". Experimental and Therapeutic Medicine 17, no. 1 (2019): 388-392. https://doi.org/10.3892/etm.2018.6912
Copy and paste a formatted citation
x
Spandidos Publications style
Yan X, Sun J and Yuan K: miR-138 expression in oral herpes simplex and its effect on ICP0. Exp Ther Med 17: 388-392, 2019.
APA
Yan, X., Sun, J., & Yuan, K. (2019). miR-138 expression in oral herpes simplex and its effect on ICP0. Experimental and Therapeutic Medicine, 17, 388-392. https://doi.org/10.3892/etm.2018.6912
MLA
Yan, X., Sun, J., Yuan, K."miR-138 expression in oral herpes simplex and its effect on ICP0". Experimental and Therapeutic Medicine 17.1 (2019): 388-392.
Chicago
Yan, X., Sun, J., Yuan, K."miR-138 expression in oral herpes simplex and its effect on ICP0". Experimental and Therapeutic Medicine 17, no. 1 (2019): 388-392. https://doi.org/10.3892/etm.2018.6912
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